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Long-acting recombinant human follicle stimulating hormone and preparation method and application thereof

A technology of restriction sites and fusion proteins, applied in the field of biomedicine, can solve the problems of changing FSH, unclear immunogenicity spectrum, etc., and achieve the effect of prolonging in vivo life, long half-life and high biological activity

Inactive Publication Date: 2019-06-28
上海延立生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this strategy may alter the structure of FSH and trigger an immune response in the body
These analogs were not tested in clinical studies and their immunogenicity profile is unknown

Method used

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  • Long-acting recombinant human follicle stimulating hormone and preparation method and application thereof
  • Long-acting recombinant human follicle stimulating hormone and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Refer to attached figure 1 , two CTP sequences were ligated to the carboxyl terminus of the FSHβ subunit by overlapping PCR method. Under the action of the LTR / CMV promoter, the chimeric gene was sequenced and cloned into a eukaryotic expression vector. Amino acid sequences of modified FSHα and FSHβ subunits containing the CTP amino acid sequence are presented (B). Cloning vectors containing FSHα and modified FSHβ subunits were co-transfected into CHO for recombinant protein production.

[0033] The preparation method is as follows:

[0034] S1. Construct FSHβ-CTP;

[0035] (1) Use the FSHβ gene as a template for primer 1 and primer 2, wherein primer 1 contains a restriction site for the 5' end sequence of FSHβ, and primer 2 contains the first 4 base codes of CTP and the last 4 codes of the 3' end of the FSHβ sequence base code;

[0036] (2) Use the hCGβ-CTP gene as the template of primer 3 and primer 4, wherein, primer 3 contains the last 4 base codes of the 3' en...

Embodiment 2

[0047] Refer to attached figure 2 , The CTP sequence was linked to the N-terminus of FSHα and the carboxyl-terminus of the FSHβ subunit by overlapping PCR method. Under the action of the LTR / CMV promoter, the chimeric gene was sequenced and cloned into a eukaryotic expression vector. The amino acid sequences of the FSHα and FSHβ subunits containing the CTP amino acid sequence are presented (B). Cloning vectors containing modified FSHα and FSHβ subunits were co-transfected into CHO for recombinant protein production.

[0048] The preparation method is as follows:

[0049] S1. Construct FSHβ-CTP;

[0050] (1) Use the FSHβ gene as a template for primer 1 and primer 2, wherein primer 1 contains a restriction site for the 5' end sequence of FSHβ, and primer 2 contains the first 4 base codes of CTP and the last 4 codes of the 3' end of the FSHβ sequence base code;

[0051] (2) Use the hCGβ-CTP gene as the template of primer 3 and primer 4, wherein, primer 3 contains the last 4...

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Abstract

The invention provides recombinant hFSH-Fc fusion protein. The fusion protein is dimerized fusion protein, wherein one monomer amino acid sequence sequentially comprises an hFSH beta sub-unit, CTP, CTP and an hFSH alpha sub-unit from the end N to the end C, and the other monomer amino acid sequence sequentially comprises CTP, an hFSH alpha sub-unit, an hFSH beta sub-unit and CTP from the end N tothe end C. The two pieces of CTP are connected to an encoding sequence of an FSH, regions important to receptor binding or bio-activity are avoided, the affinity of receptor binding is not affected, immunogenicity is avoided, and the volume lifetime can be remarkably prolonged. Meanwhile, the in-vivo half-life period of FSH-CTP is longer, the ending half-life period is 2-3 times that of recombinant FSH-WT, one time of injection per week can replace seven times of injection every day, and the bio-activity is higher.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a long-acting recombinant human follicle-stimulating hormone. Background technique [0002] At present, the infertility rate in the world is as high as 15%, becoming the third major disease seriously affecting human health after cancer and cardiovascular and cerebrovascular diseases. The number of infertility in my country accounts for more than 10% of the number of reproductive women, and its incidence is on the rise. Human follicle-stimulating hormone (hFSH for short) is the main component of common drugs used to treat male and female infertility in the market. [0003] Human follicle-stimulating hormone (hFSH) is a gonadotropin secreted by the anterior pituitary gland and is necessary for normal reproductive function in both males and females. Follicle-stimulating hormone, luteinizing hormone (LH), human chorionic gonadotropin (hCG), and thyrotropin (TSH) together consti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/62C12N15/85A61K38/24A61P15/08
Inventor 巴塞姆·法瑞斯
Owner 上海延立生物技术有限公司
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