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Preparation method of selenium-enriched health-care products

A health care product and selenium-enriching technology, which is applied in botany equipment and methods, functions of food ingredients, food ingredients, etc., can solve problems such as poor selenium-enriching effect, and achieve the effect of improving production efficiency and increasing selenium content

Inactive Publication Date: 2019-07-02
天津市先盈发科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, usually only one kind of edible fungi is used, and the effect of enriching selenium is not strong.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Prepare medium: 20g sucrose, 20g crushed black soybeans, 70g wheat, 70g yeast extract, 2g vitamin A, 5g magnesium sulfate, 10g potassium dihydrogen phosphate, 700mL water supplement, stir, and finally add to a final concentration of 20μg / L sodium selenite, pH natural;

[0017] 2) After sterilizing and cooling, insert the mixed liquid seeds of Lentinus edodes seeds and Ganoderma lucidum seeds according to the mass volume ratio of 5% (in the mixed liquid seeds of the Lentinus edodes seeds and Ganoderma lucidum seeds, the liveness of Lentinus edodes seeds and Ganoderma lucidum seeds The ratio of the number of bacteria is 1:2), the temperature is 25°C, the ventilation is stirred, and the fermentation time is 24 hours;

[0018] Treat the cultured fermentation broth with a 2000rpm centrifuge, collect mycelia, wash and freeze-dry to obtain intracellular selenium, concentrate the fermentation broth by ultrafiltration, extract with ethanol, collect solids after centrifugation...

Embodiment 2

[0020] 1) Preparation of medium: 50g of sucrose, 50g of crushed black beans, 70g of wheat, 70g of yeast extract, 2g of vitamin A, 5g of magnesium sulfate, 10g of potassium dihydrogen phosphate, 700mL of water, stirring, and finally adding a final concentration of 50μg / L sodium selenite, pH natural;

[0021] 2) After sterilization and cooling, insert the mixed liquid seeds of Lentinus edodes seeds and Ganoderma lucidum seeds according to the mass volume ratio of 10% (in the mixed liquid seeds of the Lentinus edodes seeds and Ganoderma lucidum seeds, the liveness of the Lentinus edodes seeds and Ganoderma lucidum seeds is The ratio of the number of bacteria is 2:1), the temperature is 27°C, the ventilation is stirred, and the fermentation time is 60 hours;

[0022] 3) Treat the cultured fermentation broth with a 10,000rpm centrifuge, collect mycelia, wash and freeze-dry to obtain intracellular selenium, concentrate the fermentation broth by ultrafiltration, extract with ethanol,...

Embodiment 3

[0024] 1) Preparation of medium: 41g of sucrose, 37g of crushed black soybeans, 70g of wheat, 70g of yeast extract, 2g of vitamin A, 5g of magnesium sulfate, 10g of potassium dihydrogen phosphate, 700mL of water, stirring, and finally adding a final concentration of 28μg / L sodium selenite, pH natural;

[0025] 2) After sterilization and cooling, insert the mixed liquid seeds of Lentinus edodes seeds and Ganoderma lucidum seeds by mass volume ratio 7% (in the mixed liquid seeds of the Lentinus edodes seeds and Ganoderma lucidum seeds, the mixture of Lentinus edodes seeds and Ganoderma lucidum seeds The ratio of the number of viable bacteria is between 1:2-2:1), the temperature is 25-27°C, aeration and stirring, and the fermentation time is 24-60 hours;

[0026] 3) Treat the cultured fermentation broth with a 6000rpm centrifuge, collect mycelia, wash and freeze-dry to obtain intracellular selenium, concentrate the fermentation broth by ultrafiltration, extract with ethanol, coll...

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PUM

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Abstract

The invention relates to a preparation method of selenium-enriched health-care products. The preparation method comprises the following steps of preparing a culture medium from cane sugar, black beansand the like, adding 20-50 [mu]g / L of sodium selenite, and regulating the pH to be natural; performing sterilizing and cooling, and then performing inoculating with edible mushroom strains and ganoderma lucidum liquid seeds according to the percentage by mass of 5%-10%, wherein the temperature is 25-27 DEG C; performing air stirring, wherein the fermentation time is 24-60 hours; and treating thecultured fermentation liquid with a centrifugal machine, collecting mycelia, performing washing and freeze drying to obtain intracellular selenium-containing substances, performing ultrafiltration with fermentation liquid, performing concentration, performing alcohol extraction with ethanol, performing centrifugation, collecting solid, then performing freeze drying to obtain extracellular selenium-containing substances, and uniformly mixing the intracellular selenium-containing substances with the extracellular selenium-containing substances to obtain products. The edible mushroom strains areadopted for fermentation, so that the production efficiency of the selenium-enriched products is improved; the edible mushroom strains and the ganoderma lucidum strains are used for jointed fermentation, so that special selenium-enriched effect can be achieved. The intracellular selenium-containing substances and the extracellular selenium-containing substances are collected, so that the seleniumcontent of the product can be increased.

Description

technical field [0001] The invention relates to the field of health care products, in particular, the invention relates to a preparation method of selenium-enriched health care products. Background technique [0002] Selenium (Se) is an essential trace element for the human body and is closely related to human health. Selenium deficiency can lead to a variety of diseases, such as muscular dystrophy, edema, anemia, premature aging, heart disease, liver necrosis, pancreatic atrophy, diabetes and a series of diseases, and lead to "Keshan disease" and "Kashin-Beck disease" and other endemic diseases, therefore, selenium is praised by scientists as the "miraculous element" of life. At present, there are more than 40 countries in the world who are deficient in selenium or lack selenium. There are also 22 provinces and cities in China, accounting for more than 72% of the counties and cities, which lack selenium. Supplementing selenium is a universal problem for the human body. St...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/16A23L31/00A01G18/40A01G18/20
CPCA23L31/00A23L33/16A23V2002/00A23V2250/1626A23V2200/30
Inventor 周会兰
Owner 天津市先盈发科技有限公司
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