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A kind of samara oil sterile seedling ga 3 Rooting medium and preparation method thereof

A technology of rooting medium and sterile seedlings, which is applied in the directions of biochemical equipment and methods, botany equipment and methods, horticultural methods, etc., can solve the problems of low rooting rate of seedlings, increase rooting rate and number of roots, reduce Effects of losses, good economic benefits, and social benefits

Active Publication Date: 2022-06-17
西安同人农林实业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the seedling rooting rate of this method is on the low side, and the maximum rooting rate is 60%.

Method used

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  • A kind of samara oil sterile seedling ga <sub>3</sub> Rooting medium and preparation method thereof
  • A kind of samara oil sterile seedling ga <sub>3</sub> Rooting medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The establishment process of the test-tube seedlings of the oil tree of the present invention: (1) material sampling; (2) disinfection, inoculation and culture; (3) expansion and propagation; (4) induction of roots.

[0026] According to the establishment process of the above test-tube plantlets, the medium suitable for rooting was screened.

[0027] The three hormones in the rooting medium were indole butyric acid (IBA), naphthalene acetic acid (NAA) and gibberellin (GA). 3 ), the rooting rate with hormones was significantly higher than that without hormones. The screening method of the added concentration of hormones: keep the other components except the hormones unchanged, keep the concentrations of the two hormones unchanged each time, and set a concentration gradient for the remaining hormones. For example, a concentration gradient is set for naphthalene acetic acid, and the concentration of other hormones is kept constant. Similarly, set concentration gradients ...

Embodiment 2-15

[0039] The rooting medium cultivates the aseptic seedling tissue of pomegranate oil, wherein, the components of each liter (L) of the rooting medium and the content of each component as well as the rooting rate and the average number of roots are shown in the following table:

[0040] Table 1 Design scheme, rooting rate and average number of roots for cultivating aseptic seedling tissue of pomegranate oil in rooting medium

[0041]

[0042] Weigh the agar powder according to the raw material ratio in the above table Example 2-15 respectively and add it to the pot containing 0.75 liters of pure water and boil; get 0.1 liters of 10 times 1 / 2MS mother liquor and indole butyric acid, naphthalene acetic acid, potato juice , after the gibberellin is dissolved, add it to the pot; weigh the sucrose into the pot and let it melt; make up to 1.0 liters of purified water; use hydrochloric acid and sodium hydroxide to adjust the pH value to 5.7; Sterilize at 120°C, high pressure 0.1MPa ...

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Abstract

The invention discloses a samara oil sterile seedling GA 3 A rooting medium and a preparation method thereof belong to the technical field of tissue culture and forest plant rapid propagation and detoxification. The components of each liter of rooting medium and the content of each component are: 1 / 2MS, indolebutyric acid 0.2-1.0mg / L, naphthaleneacetic acid 0.005-0.03mg / L, gibberellin 0.1-0.5mg / L, Potato juice 100-200g / L, sucrose 10-30g / L, agar powder 2-10g / L, pure water to make up the balance. This technical solution optimizes the various components of the medium, especially adding and adjusting the concentration of hormones indole butyric acid, naphthalene acetic acid and gibberellin in the medium, so that the medium is more conducive to the rooting of samara oil sterile seedlings Induction, aseptic seedlings have high rooting rate, fast rooting, well-developed root system, good lignification of seedlings, and high survival rate of transplanting. The invention has the advantages of simple formula, convenient use and good effect.

Description

technical field [0001] The invention relates to the technical field of tissue culture and rapid propagation and detoxification of forest plants, in particular to a sterile seedling GA of pomegranate oil 3 Rooting medium and preparation method thereof. Background technique [0002] Plant test-tube seedling technology is aseptic tissue culture technology. The principle of tissue culture medium and cell totipotency can dedifferentiate plant tissues that have been at the terminal of differentiation or are in the process of differentiation, induce the formation of callus, and then form new clusters on the callus. bud. That is, under artificially created sterile conditions, the living isolated organs (such as roots, stems, leaves, stem segments, protoplasts), tissues or cells are placed in the culture medium, and placed in a suitable environment for continuous culture. Techniques to obtain cells, tissues or individuals. It is widely used in improving crops, propagating plants a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00
CPCA01H4/001
Inventor 王小帆史玮张吉傲笛
Owner 西安同人农林实业股份有限公司