Microbial remediation method for tailing pond heavy metal pollution

A technology for microbial remediation and heavy metals, applied in the field of bioremediation, can solve the problems of difficult application of heavy metal pollution sites, easy to change soil properties, and plant yield reduction, etc. Effect

Active Publication Date: 2019-07-05
有研资源环境技术研究院(北京)有限公司
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AI Technical Summary

Problems solved by technology

Due to the small area of ​​heavy metal pollution in foreign countries, the combination of chemical and physical treatment is mainly adopted after ectopic chemical solidification and then landfill. This repair method is very expensive.
At present, the in-situ chemical solidification technology is mainly used in China. The biggest problem is that the cost is too high, the soil properties are easily changed, and the plant production is reduced.
It should also be emphasized that the above methods are only for sites that have been polluted by heavy metals (no new sources of heavy metal pollution), and it is difficult to apply to the pollution sites where heavy metals are continuously leached out (non-ferrous metal mine tailings)

Method used

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  • Microbial remediation method for tailing pond heavy metal pollution
  • Microbial remediation method for tailing pond heavy metal pollution
  • Microbial remediation method for tailing pond heavy metal pollution

Examples

Experimental program
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Effect test

Embodiment 1

[0034] EXAMPLE 1 Lab-Scale Restoration

[0035] The experiment was set up in the restoration group and the control group. In the restoration group, 120L of the mixed culture solution of sulfate-reducing bacteria EM2, SRB-4 and iron-ion-reducing 7 CFU / mL) was sprayed on the surface of about 0.5 tons of tailings, while the control group was only sprayed with an equal volume of water. The two groups were placed in an environment of 30°C for 60 days, the exudate was collected, and finally the physical, chemical and microbiological tests were carried out.

[0036] The pH value and redox potential value of the seepage were measured with a pH meter and redox potential electrode, and the seepage was filtered with an ultrafiltration membrane (pore size 0.2 μM) and analyzed for soluble elements by ICP-OES technology.

[0037] Analysis of microbial population structure by high-throughput sequencing. The samples to be tested were collected by centrifugation, and the whole genome DNA of ...

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Abstract

The invention provides a method for tailing pond microbial remediation with multiple sulfate-reducing bacteria EM2, SRB-4 and iron ion reducing bacteria Biometek-A. The above bacteria are introduced into tailings and nutrients needed by microbial growth are added to construct a set of tailing pond microbial remediation system. Some nutrients added into the tailings and growth of the reducing bacteria can inhibit the growth of inherent oxidizing bacteria in tailings and obviously inhibit the oxidation and dissolution of multiple heavy metal ions, thus improving the pH of tailings, lowering theredox potential, and at the same time greatly reducing the permeability of tailings. Therefore, the remediation method can reach a remediation effect on the tailing pond.

Description

technical field [0001] The invention belongs to the field of bioremediation, and in particular relates to a microbial remediation method for heavy metal pollution in tailing ponds by applying a variety of sulfate-reducing bacteria and iron ion-reducing bacteria to tailings heavy metal pollution. Background technique [0002] At present, the remediation methods of heavy metal pollution in tailings ponds are mainly divided into three categories: physical methods, chemical methods and biological methods. Due to the small area of ​​heavy metal pollution in foreign countries, the combination of chemical and physical treatment is mainly adopted after ectopic chemical solidification and then landfill. This restoration method is very costly. At present, the in-situ chemical solidification technology is mainly used in China. The biggest problem is that the cost is too high, the soil properties are easily changed, and the plant production is reduced. It should also be emphasized that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 张明江刘兴宇李益斌闫潇
Owner 有研资源环境技术研究院(北京)有限公司
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