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High-accuracy pepsinogen II detection kit

A pepsinogen and detection kit technology, applied in measurement devices, instruments, scientific instruments and other directions, can solve the problems of long measurement time, complicated operation process, and few detection samples, and achieve fast and convenient detection, high detection accuracy, The effect of improving stability

Inactive Publication Date: 2019-07-05
芜湖森爱驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the enzyme-linked immunosorbent assay, its detection accuracy is high, but the operation process is slightly complicated, the measurement time is long, and there are many influencing factors, and it is not suitable for the detection of emergency samples; in the chemiluminescence immunoassay assay, its automatic operation Fast speed and high accuracy, but high cost, expensive equipment, and poor stability, so it cannot be popularized in middle-level and grass-roots hospitals and medical institutions; in the latex immunoturbidimetric assay, its detection is fast, accurate, accurate, and specific , good precision, low cost, and a small amount of testing samples, which can be used for the determination of a small number of samples and emergency samples
[0005] However, the existing latex immunoturbidimetric method has some shortcomings: poor stability, poor sensitivity, low accuracy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A high-accuracy pepsinogen II detection kit, including independent reagents R1 and R2, the reagent R1 is composed of the following substances: MES-NaOH buffer solution 80mmol / L; Triton X-100 1.2g / L; PEG-4000 10g / L; p-hydroxybenzoic acid 1g / L; fish skin gelatin protein 20g / L;

[0039] The reagent R2 is composed of the following substances: MES-NaOH buffer solution 120mmol / L; fish skin gelatin protein 4mg / L; pepsinogen II antibody 2g / L; polystyrene latex particles 5g / L; sodium azide 1g / L ;

[0040] The preparation method of described reagent R2 comprises the following steps:

[0041] Dilute 0.5g carboxylated polystyrene latex microsphere solution with 100mL MES-NaOH buffer (120mmol / L), then add 0.01moL N-hydroxysuccinimide, 0.05mol 1-(3-dimethylaminopropyl Base)-3-ethylcarbodiimide hydrochloride activated carboxylated latex microspheres, ultrasonically dispersed for 45min, then added 0.2g of anti-human pepsinogen Ⅱ (wherein, mouse pepsinogen Ⅱ monoclonal antibody and m...

Embodiment 2

[0043] A high-accuracy pepsinogen II detection kit, including independent reagent R1 and reagent R2, the reagent R1 is composed of the following substances: MES-NaOH buffer 100mmol / L; Tween 20 2g / L; PEG- 8000 8g / L; p-hydroxybenzoic acid 2g / L; disodium edetate 15g / L;

[0044] The reagent R2 is composed of the following substances: HEPPS-NaOH buffer solution 120mmol / L; ovalbumin 4.5mg / L; pepsinogen II antibody 1.5g / L; polystyrene latex particles 8g / L; Esters 1.5g / L;

[0045] The preparation method of described reagent R2 comprises the following steps:

[0046]Dilute 0.8g carboxylated polystyrene latex microsphere solution with 100mL MES-NaOH buffer (120mmol / L), then add 0.05mol N-hydroxysuccinimide, 0.08mol 1-(3-dimethylaminopropyl base)-3-ethylcarbodiimide hydrochloride to activate carboxylated latex microspheres, ultrasonically disperse for 45min, then add 0.15g of anti-human pepsinogen Ⅱ (among them, mouse pepsinogen Ⅱ monoclonal antibody and mouse pepsinogen Ⅱ monoclonal a...

Embodiment 3

[0048] A high-accuracy pepsinogen II detection kit, including independent reagents R1 and R2, the reagent R1 is composed of the following substances: HEPPS-NaOH buffer 50mmol / L; Tween 20 0.5g / L; PEG -4000 12g / L; sodium azide 0.05g / L; ovalbumin 30g / L;

[0049] The reagent R2 is composed of the following substances: HEPPS-NaOH buffer solution 150mmol / L; ovalbumin 2mg / L; pepsinogen II antibody 0.5g / L; polystyrene latex particles 3g / L; 1.2g / L;

[0050] The preparation method of described reagent R2 comprises the following steps:

[0051] Dilute 0.3g carboxylated polystyrene latex microsphere solution with 100mL HEPPS-NaOH buffer (150mmol / L), then add 0.02moL N-hydroxysuccinimide, 0.03mol 1-(3-dimethylaminopropyl base)-3-ethylcarbodiimide hydrochloride to activate carboxylated latex microspheres, ultrasonically disperse for 30min, then add 0.05g of anti-human pepsinogen Ⅱ (among them, mouse pepsinogen Ⅱ monoclonal antibody and mouse pepsinogen Ⅱ monoclonal antibody The ratio of ...

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Abstract

The invention relates to the technical field of biological detection technology, in particular to a high-accuracy pepsinogen II detection kit. The high-accuracy pepsinogen II detection kit comprises areagent R1 and a reagent R2 which are mutually independent. The reagent R1 consists of the following substances: 35-120 mmol / L of a buffer solution 1, 0.05-3 g / L of a surfactant, 8-12 g / L of a coagulant, 0.05-2 g / L of a preservative 1 and 15-30 g / L of a stabilizer. The reagent R2 consists of the following substances: 100-150 mmol / L of a buffer solution 2, 2-5 mg / L of a blocker, 0.5-3 g / L of a pepsinogen II antibody, 1-12 g / L of polystyrene latex particles, and 0.5-2 g / L of a preservative 2. The pepsinogen II detection kit provided by the invention has the advantages of high detection accuracyrate and good stability.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a high-accuracy pepsinogen II detection kit. Background technique [0002] Pepsinogen (Pepsinogen, PG) is an inactive precursor of pepsin in gastric juice, a single-chain polypeptide with a molecular weight of 42000Da, which can be converted into active pepsin in the stomach. According to biochemical properties, immunogenicity, cell origin and tissue distribution, pepsinogen can be divided into two subgroups, pepsinogen Ⅰ (PGⅠ) and pepsinogen Ⅱ (PGⅡ). It is called PGⅠ (PGA), which is mainly secreted by the principal cells of the fundic gland and cervical mucous cells; 6-7 components are called PGⅡ (PGC), except that it is secreted by the above two cells, the mucus cells of the pyloric gland, cardia Gland and the Brunner gland in the upper part of the duodenum can also produce PGII, and the PGII synthesized by the gastric mucosa is about 25% of the total. [0003] S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/577
CPCG01N33/573G01N33/577
Inventor 王晓波朱雪姣朱海峰
Owner 芜湖森爱驰生物科技有限公司