Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring content of metabolic products of N'-Nitrosonornicotine(NNN) in liver tissue and predicting N'-Nitrosonornicotine (NNN) exposure risk

A technology of exposure risk and liver tissue, applied in the field of biochemical analysis, to achieve the effect of accurate evaluation results

Inactive Publication Date: 2019-07-09
ZHENGZHOU TOBACCO RES INST OF CNTC
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no quantitative index to directly evaluate the potential toxic activity of NNN from the perspective of metabolic analysis, and a method to evaluate the exposure risk of NNN based on the quantitative index

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring content of metabolic products of N'-Nitrosonornicotine(NNN) in liver tissue and predicting N'-Nitrosonornicotine (NNN) exposure risk
  • Method for measuring content of metabolic products of N'-Nitrosonornicotine(NNN) in liver tissue and predicting N'-Nitrosonornicotine (NNN) exposure risk
  • Method for measuring content of metabolic products of N'-Nitrosonornicotine(NNN) in liver tissue and predicting N'-Nitrosonornicotine (NNN) exposure risk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In the method for measuring the content of NNN metabolites in liver tissue in this embodiment, two healthy breeds of adult Japanese big-eared white rabbits were selected as the exposed animals, numbered A and B respectively, with a body weight of 2.5 to 3.0 kg. Independent isolated breeding cages are raised, and the method comprises the following steps:

[0043] 1. Blood sample processing and NNN direct metabolite content determination

[0044] (1) Processing of blood samples

[0045] Rabbits were fasted overnight before the experiment, and NNN solution (injection dose: 0.8 mg / kg, prepared with normal saline) was injected into ear margin vein the next day. At different time points of 5, 15, 30, 60, 120, 240, and 360 minutes after exposure, about 0.5 mL of blood was collected from the vein and placed in a 1.5 mL anticoagulant centrifuge tube, and centrifuged at 2000 g for 10 minutes at 4 ° C. Put 100 μL of supernatant into a 1.5 mL centrifuge tube, add 20 μL of mixed i...

Embodiment 2

[0062] In this example, the method for determining the content of NNN metabolites in liver tissue selected two groups of C57BL / 6 healthy mature mice and randomly divided them into a normal group and an alcoholic fatty liver group (hereinafter referred to as the alcohol group), with 5 mice in each group. The mice in the alcohol group were fed with a high-fat and high-sugar feed, including 83% common feed, 2% cholesterol, 10% lard, 5% sucrose in mass percent, and freely drank alcoholic beverages [15% (mass ratio) Sucrose aqueous solution+alcohol], its alcohol concentration is 5%, 10%, 15%, 20%, 25%, 30%, 35% and 40% increase sequentially according to volume ratio, and maintain 2 weeks at 40%. Mice in the normal group were fed with common feed and drank water freely. The mice in the two groups were fed ad libitum for 8 weeks.

[0063] 1. Blood sample processing and NNN direct metabolite content determination

[0064] (1) Processing of blood samples

[0065] The mice were faste...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for measuring the content of metabolic products of N'-Nitrosonornicotine(NNN) in liver tissue and predicting a NNN exposure risk, and belongs to the technical field of biochemical analysis. According to the method for measuring the content of the metabolic products of NNN in the liver tissue and predicting the NNN exposure risk, an alpha-hydroxylation reaction degree in different individual bodies after the NNN is exposed is evaluated through the measurement of direct metabolic products of NNN in blood and indirect metabolic products of the NNN in the liver tissue, based on the acquirement of into-systemic-circulation relative amount of the NNN and the metabolic products of the NNN, and separately according to the ratio of the total amount of alpha-hydroxylation metabolic pathway products in blood to the relative amount of the NNN entering into the systemic circulation and the output of the representative DNA adduct in main metabolic organs. Accordingto the method for measuring the content of the metabolic products of the NNN in the liver tissue and predicting the NNN exposure risk, the evaluation result is accurate, reliable and comparable, and anew method capable of being used for evaluating NNN primary exposure risk is created.

Description

technical field [0001] The invention relates to a method for measuring the content of NNN metabolites in liver tissue and predicting the risk of NNN exposure, belonging to the technical field of biochemical analysis. Background technique [0002] TSNAs are unique N-nitrosamine harmful components in tobacco, among which N'-nitrosonornicotine (NNN) is also listed as Class I human carcinogen by the International Agency for Research on Cancer (IARC), which has a strong organ Carcinogenic activity, such as related to the occurrence of lung cancer, pancreatic cancer and other cancers. At present, it is believed that the carcinogenic effect of TSNAs is produced through its metabolic activation in vivo, so it is particularly necessary to study the metabolic process of TSNAs in vivo. [0003] Studies have shown that there are three main pathways for the metabolism of NNN in the body, the first is pyridine-N-oxidation, the second is the hydroxylation of the pyridine ring (including α...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72
Inventor 毛健张建勋张启东柴国璧刘俊辉宋瑜冰史清照范武席辉孙世豪洪广峰宗永立
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com