Method for investigating quality of characterization natural cordyceps sinensis character
A technology for quality evaluation of Cordyceps sinensis, applied in the field of Cordyceps quality evaluation to characterize the characteristics of natural Cordyceps sinensis, can solve the problems of insufficient quality evaluation methods of Cordyceps, affecting the safety and effectiveness of Cordyceps quality evaluation, etc.
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[0035] Example 1:
[0036] Sample preparation: Take about 0.5g of natural cordyceps sample powder in a 50ml tube with stopper, ① add 10ml of deionized water accurately, weigh it, and mix well. After standing for 18 hours at room temperature, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml methanol, weigh, mix, sonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45μm filter membrane and then used for HPLC analysis.
[0037] HPLC analysis: Agilent 1100 series high performance liquid chromatograph, equipped with quaternary pump, automatic sampling system, diode array detector (DAD) and Agilent chromatography workstation. The chromatographic column is Zorbax SB-C18 (250×4.6mm i.d., 5μm) analytical column and Zobax SB-C18 (12.5×4.6mm i.d., 5μm) pre-column. The elution conditions are gradient elution with water (containing 10 mM triethylamine) (A) and methanol (B): 0-30 minutes, gradient elution from 0% B to 30% B. The...
Example Embodiment
[0039] Example 2:
[0040]Sample preparation: Take about 0.5g of the artificial cordyceps sample powder in a 50ml tube with stopper, ① add 10ml of deionized water accurately, weigh it, and mix well. After standing for 18 hours at room temperature, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml methanol, weigh, mix, sonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45μm filter membrane and then used for HPLC analysis.
[0041] HPLC analysis: the same as in Example 1.
[0042] For the measurement results, refer to Figure 3 and Figure 4: Determination of 9 batches of artificial cordyceps taken from Jiangxi, Hebei, Zhejiang and Anhui. The content ratios of 5 components of uridine, inosine, guanosine, adenosine and cordycepin were determined. The detection data of the warm water ultrasonic treatment sample is: 100:0~20:40~160:30~140:0~10, in which the uridine content is 1.0~4.0mg / g; the detection data of the methan...
Example Embodiment
[0043] Example 3:
[0044] Sample preparation: Take about 0.5g of the artificial Cordyceps militaris sample powder into a 50ml tube with a stopper, ① accurately add 10ml of deionized water, weigh it, and mix well. After standing for 18 hours at room temperature, sonicate for 30 minutes, make up the weight, and centrifuge; ②Add 10ml methanol, weigh, mix, sonicate for 30 minutes, make up the weight, and centrifuge; the supernatant is filtered with a 0.45μm filter membrane and then used for HPLC analysis.
[0045] HPLC analysis: the same as in Example 1.
[0046] For the measurement results, refer to Figure 5 and Figure 6: The content ratios of 7 batches of artificial Cordyceps militaris collected from Jiangsu, Shanghai, Guangdong and Liaoning were determined, and the content ratios of uridine, inosine, guanosine, adenosine and cordycepin were determined. The detection data of the room temperature water ultrasonic treatment sample is 100:0~10:20~100:50~90:200~800, in which the uridin...
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