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Application of inactivated plasmodium to preparing medicine for treating cancer

A technology of Plasmodium and Plasmodium falciparum, applied in the field of biomedicine, can solve the problems of limited research or new applications of Plasmodium, and achieve the effects of enhancing anti-tumor immune response, high safety, and prolonging the survival period

Pending Publication Date: 2019-07-12
BLUE ELEGANT BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] But so far, the research or new application of malaria parasites, especially inactivated malaria parasites, is still very limited, so it is very meaningful to develop a new application method of inactivated malaria parasites

Method used

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  • Application of inactivated plasmodium to preparing medicine for treating cancer
  • Application of inactivated plasmodium to preparing medicine for treating cancer
  • Application of inactivated plasmodium to preparing medicine for treating cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation of Inactivated Plasmodium Suspension and Red Blood Cell Suspension

[0037] (1) Preparation of inactivated Plasmodium suspension

[0038] The Plasmodium strain used was Plasmodium falciparum PfNF54 strain, which was derived from The Malaria Research and Reference Reagent Resource Center (MR4), and the Plasmodium was continuously cultured according to the existing mature conventional method.

[0039] The specific method is:

[0040] (I) Transfer the Plasmodium cultured in vitro (infection rate>8%, in the stage of large trophozoite and schizont) to a centrifuge tube, centrifuge at 300g for 5min, and discard the supernatant;

[0041] (II) Add twice the volume of 1640 medium to resuspend, centrifuge at 300g for 5 minutes, discard the supernatant, take out a small part of the blood smear, and calculate the infection rate to be above 90%.

[0042] (Ⅲ) Add 3 mL of 70% Percoll separation solution to a 15 mL centrifuge tube, and slowly and carefully add 1 mL of the a...

Embodiment 2

[0049] Safety Evaluation Test

[0050] (1) Experimental animals and materials: C57BL / 6J mice, female, 4 weeks old, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.; inactivated Plasmodium Pf NF54 suspension; broken red blood cell suspension, Human type O red blood cells; 75% ethanol; normal saline; 1ml insulin syringe, etc.

[0051] (2) Raising conditions of experimental animals: All mice were free to forage for food and drink water, room temperature (23±2)°C, and were raised in Guangzhou Yuansheng Pharmaceutical Technology Co., Ltd.; the feed and water were autoclaved, and all experimental feeding The process and experiments were carried out in accordance with relevant experimental standards.

[0052] (3) Experimental steps:

[0053] (I) Take 9 C57BL / 6J mice and divide them into 0.9% sodium chloride group, erythrocyte suspension group and inactivated malaria parasite suspension group, totally 3 groups. Inject normal saline, red blood cell suspe...

Embodiment 3

[0067] Pharmacodynamic evaluation test on mouse model of colon cancer

[0068] (1) Experimental animals and materials: Balb / c mice, female, 4 weeks old, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.; inactivated Plasmodium suspension; red blood cell suspension; mouse colon cancer Cell C26 was obtained from the American Type Culture Collection.

[0069] (2) Raising conditions of experimental animals: All mice were free to forage for food and drink water, room temperature (23±2)°C, and were raised in Guangzhou Yuansheng Pharmaceutical Technology Co., Ltd.; the feed and water were autoclaved, and all experimental feeding The process and experiments were carried out in accordance with relevant experimental standards.

[0070] (3) Experimental steps:

[0071] (I) get 30 Balb / c mice, divide into 0.9% sodium chloride group; Red blood cell suspension group; Inactivated Plasmodium suspension group, altogether 3 groups;

[0072] (II) C26 cells were cul...

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Abstract

The invention relates to application of inactivated plasmodium to preparing medicine for treating cancer. According to the application, a high-purity inactivated-plasmodium antigen mixture is obtainedthrough density gradient centrifugation and ultrasonication, and serves as an antigen for adjusting activities of host dendritic cells and natural killer cells, a series of immunologic effector cellssuch as T cells and B cells are activated, a systematic immune response of host is comprehensively activated, an antitumor immunity response of a main body can be enhanced, and the inactivated plasmodium has the potential antitumor activity, and provides new strategy and thinking for cancer of treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of inactivated malaria parasites, in particular to an application of inactivated malaria parasites in the preparation of medicines for treating cancer. Background technique [0002] Colorectal cancer, also known as colorectal cancer, rectal cancer or bowel cancer, is a cancer originating from the colon or rectum and is a common malignant tumor in the gastrointestinal tract. The etiology and mechanism of colorectal cancer need to be further studied. It can occur in any part of the colon or rectum, but the rectum and sigmoid colon are the most common, and the rest are found in the cecum, ascending colon, descending colon, and transverse colon in turn. Most of the cancers are adenocarcinoma, and a few are squamous cell carcinoma and mucinous carcinoma. So far, the radical treatment method is still the first choice of surgical treatment, but there are many patients with peri...

Claims

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Application Information

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IPC IPC(8): A61K39/015A61P35/00
CPCA61K39/015A61P35/00A61K2039/57A61K2039/521Y02A50/30
Inventor 刘玉成侯永恒蔡玉梅黄振平许仁朝丘润敏陈沛泉秦莉陈小平
Owner BLUE ELEGANT BIOTECH CO LTD
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