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Method of directly and rapidly detecting uric acid

A uric acid and fast technology, applied in the direction of measuring devices, instruments, and material analysis through electromagnetic means, can solve the problems of ascorbic acid interference, poor anti-interference ability, high pH requirements, etc., achieve broad application prospects, improve work efficiency, and high sensitivity Effect

Pending Publication Date: 2019-07-16
钟士博 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The linear detection range of the above method 1 is 0-50 μmol / L, and the regression curve is fitted with the absorbance to the UA concentration, and the regression equation R 2 =0.980, the linear relationship is not very good in the concentration range of 0-50 μmol / L, so the linear detection range of this method is narrow, and the detection sensitivity is low;
[0009] The above-mentioned method 2 has a linear detection range of 178.6-714.3 μmol / L, requires a large amount of expensive enzymes, and has high pH requirements. Ascorbic acid has obvious interference on the detection of uric acid, so the linear detection range of this method is narrow and the anti-interference ability is poor. The detection cost is high;
[0010] The above method 3 requires complex sample pretreatment, expensive instruments, and professional operation;
[0011] In the above method, the sample must go through complex and cumbersome processes such as pretreatment, and the instrument is expensive and cannot be carried, and the detection time is long, which makes it difficult to meet the needs of online, sensitive and rapid detection

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  • Method of directly and rapidly detecting uric acid

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Sensor preparation and characterization: A nanoporous gold sensor with a three-electrode system was assembled using an NPG / GCE electrode as a working electrode, a platinum electrode as a counter electrode, and a saturated calomel electrode as a reference electrode; 2 SO 4 Carry out cyclic voltammetry to scan 15 circles, and characterize the effective area of ​​the NPG / GCE electrode by reducing the peak-to-peak current;

[0039] Detection of uric acid standard: 15mL pH 4.0 concentration of 100mM citric acid-sodium citrate buffer was purged with high-purity nitrogen for 5 minutes, and the prepared sensor was put into the reaction system of degassed citric acid-sodium citrate buffer In the process, add uric acid standards with final concentrations of 100 μM and 200 μM respectively, and use the sensor to detect the current response of added uric acid standards with known concentrations in the range of 0.3-0.6V; An obvious oxidation peak, the detection results are as follows ...

Embodiment 2

[0044] Sensor preparation and characterization: The prepared NPG / GCE electrode was used as the working electrode, the platinum electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode to assemble a nanoporous gold sensor with a three-electrode system; 2 SO 4 Carry out cyclic voltammetry to scan 15 circles, and characterize the effective area of ​​the NPG / GCE electrode by reducing the peak-to-peak current;

[0045] Sensor calibration or calibration: Blow 15mL pH 4.0 100mM citric acid-sodium citrate buffer solution with high-purity nitrogen for 5 minutes, put the prepared sensor into the reaction system of degassed citric acid-sodium citrate buffer solution, Add uric acid standard products with a final concentration of 10-1200 μM respectively, use the sensor to detect the current response of the added uric acid standard products with known corresponding concentrations in the interval of 0.3-0.6V, and make a concentratio...

Embodiment 3

[0050] Sensor preparation and characterization: The prepared NPG / GCE electrode was used as the working electrode, the platinum electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode to assemble a nanoporous gold sensor with a three-electrode system; 2 SO 4 Carry out cyclic voltammetry to scan 15 circles, and characterize the effective area of ​​the NPG / GCE electrode by reducing the peak-to-peak current;

[0051] Sensor calibration or calibration: 15mL pH 4.0 concentration of 100mM citric acid-sodium citrate buffer is purged with high-purity nitrogen for 5 minutes, and the prepared sensor is placed in the reaction system of degassed citric acid-sodium citrate buffer , respectively adding uric acid standard products with a final concentration of 10-1200 μM, using the sensor to detect the current response of the added uric acid standard products with known corresponding concentrations in the interval of 0.3-0.6V, and m...

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Abstract

The invention discloses a method of rapidly detecting uric acid in an actual sample by using a nanoporous gold sensor. The method comprises steps of (1) sensor preparation and characterization, (2) sensor calibration or correction and (3) uric acid detection in an actual sample. The method can effectively solve the problems that the traditional uric acid detection method is cumbersome in steps andonline detection is hard to perform, has the characteristics of simple process, accurate measurement, high specificity, rapid reaction, high sensitivity and simple operation, realizes direct detection on the actual sample and has a broad application prospect.

Description

technical field [0001] The invention relates to a method for uric acid in actual samples, in particular to a method for rapidly detecting uric acid by using a nanoporous gold sensor. It belongs to the field of electrochemical analysis and testing. Background technique [0002] In recent years, with the improvement of living standards, the incidence of hyperuricemia has increased year by year, and it is showing a younger trend. Hyperuricemia refers to a metabolic disease in which blood uric acid increases in the human body. Uric acid (Uric acid, UA), also known as 2,6,8-trihydroxypurine, is the final product of purine metabolism in the human body. The normal uric acid level of healthy adults is The level is 25-77μg / mL. If there is too much uric acid excreted in the body or the mechanism of uric acid excretion is abnormal, there will be too much uric acid in the body. Uric acid is a biomarker in the human body. People can monitor a variety of diseases through the level of ur...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48G01N27/416
CPCG01N27/30G01N27/308G01N27/48G01N27/4163
Inventor 陈毅力钟士博
Owner 钟士博
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