A kind of short-term preservation method of sunflower pollen

Abstract

The invention discloses a method for preserving sunflower pollen. The method comprises: (1) sleeving sunflower floral disc with an 80-mesh gauze bag before loosening powder; (2) disinfecting a pollencollecting device, a relay thermal insulating box and a refrigerator by using 75% alcohol in advance, and carrying out air drying so as to be spare; (3) collecting sunflower pollen from 10:00 to 13:00in fine weather, and transferring the pollen into the pollen collecting device; and (4) rapidly sealing the pollen collecting device after inflating the pollen collecting device with CO2, immediatelytransferring the pollen collecting device into the relay thermal insulating box, and transferring the pollen collecting device in the relay thermal insulating box into the 4 DEG C refrigerator withinhalf an hour. According to the present invention, the method is simple in operation, can preserve a large amount of pollen, and can meet the demand of large-scale breeding on sunflower pollen, wherein the sunflower pollen has high vigor after being preserved for 30 days, and the setting rate after pollination is above 70%.

Description

technical field [0001] The invention belongs to the technical field of plant organ preservation, and in particular relates to a short-term preservation method of sunflower pollen. Background technique [0002] Sunflower is the largest food crop in my country. The sown area and total output of sunflower have been on the rise in the past 10 years. Among the various factors of sunflower yield growth, improved varieties account for about 35% to 40%. Therefore, cultivating new varieties with high yield and high quality is the primary measure to ensure the development of sunflower industry. Л.А.Жданов et al. have clearly proved through experimental data that under the conditions of proper selection of varieties and mentors, heterologous pollen assisted pollination has a good impact on the viability and productivity of sunflower self-pollinated offspring. Furthermore, and importantly, aftereffects of heteropollens affect some shapes such as height, length of growing season and oil...

AI Technical Summary

Problems solved by technology

However, the drying process (mostly using hot air drying for more than 2 hours) itself will cause a large number of sunflower pollen cells to be damaged; and after cryopreservation, it often needs to be thawed in a water bath at 40-42°C and recovered for 24 hours before it can be used. pollen, and the thawing process will also cause damage to the pollen cells, and the pollen after freezing and thawing is easy to condense into agglomerates, and the pollen cell damage rate is high; and in order to ensure the effect of freezing, use a 1.8ml cryopreservation tube for optimal

[0008] Therefore, cryopreservation is not only complicated to operate, but also greatly increases the cost of experiments and labor intensity, and also causes considerable damage to the pollen cells themselves.

In the current breeding work, the collection of sunflower pollen is huge, and storage in cryopreservation tubes often cannot meet the needs of field experiments

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