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Combination of stem cells and cytokines, and use thereof in improving sperm activity

A technology of cytokines and sperm motility, applied in drug combinations, medical preparations containing active ingredients, peptide/protein components, etc., can solve unknown problems

Pending Publication Date: 2019-07-19
SINO AMERICAN BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this solution is mainly used to improve the fertility status of livestock, and whether it is applicable to humans is still unknown

Method used

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  • Combination of stem cells and cytokines, and use thereof in improving sperm activity
  • Combination of stem cells and cytokines, and use thereof in improving sperm activity
  • Combination of stem cells and cytokines, and use thereof in improving sperm activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Peripheral blood stem cell PBMC preparation

[0043] Daily intramuscular injection of mobilizing agent (recombinant human granulocyte-stimulating factor GCSF), the dose is 5-10ug / kg / day, continuous injection of mobilizing agent for 5 days, and from the next day, 1ml of venous blood is drawn daily to monitor white blood cells. To 25.0 10^10g / L, the amount of mobilizing agent should not exceed (5ug / kg / day). After five days of GCSF injection, PBMC can be collected when the white blood cell value reaches 25.0 10^10g / L or above. Generally, the white blood cell can reach 40.0-45.0 10^10g / L, which is the "ideal mobilization state", that is, the effect of GCSF on bone marrow colonies Stimulate success.

[0044] After successful mobilization, stem cells (PBMC) were collected with a blood separator. Specifically, puncture the veins of the elbow, drain the blood into the blood separator through a closed tube, and collect it through a machine with centrifugal setting...

Embodiment 2

[0046] Embodiment 2 composition improves the effect of male sperm motility

[0047] Sperm motility was significantly positively correlated with parameters such as VSL, VCL, and VAP, and sperm motility parameters such as VSL, VCL, and VAP were routine indicators reflecting sperm motility. In this example, the effect of the composition of the present invention on improving sperm motility in men with normal sex life is investigated, and the specific method is as follows. For 16 healthy married male volunteers aged 22 to 44 with normal sex life, peripheral blood stem cells were prepared by the method in Example 1 before the experiment started, and He Jiang et al. (Chinese Journal of Eugenics and Genetics, 2010, 18(5): 110) collected semen and measured VSL, VCL, VAP value and sperm motility value (a+b%), and the result was expressed as the average value of 16 volunteers, which was recorded as the initial value; Then give all subjects the pharmaceutical composition of the present i...

Embodiment 3

[0049] Embodiment 3: Composition improves the effect of male asthenospermia

[0050] Mr. Wu, about 40 years old, weighing 70Kg, suffering from infertility. Collect PBMC according to Example 1, and add cytokine composition 0.3mgMCP-1+0.3mgGDNF+0.3mgOPG in 50mlPBMC, infuse once every two months, reinfuse three times in total, and infuse PBMC150ml altogether (it contains The number of stem cells is about 6X10 7), the date of the first return is September 18, 2016; the date of the second return is November 20, 2016; the date of the third return is January 20, 2017. The sperm motility rate was successfully improved from 1.80% (the physical examination value before reinfusion, see Table 1, where the standard value: ≥40%) to 17.6% for the second time (two months after the first reinfusion, see Table 2), and the third time 70% (two months after the second reinfusion, see Table 3). The forward rate of sperm was successfully improved from 1.58 (standard value: ≥32%, Table 1) to 14.81...

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Abstract

The present invention relates to the field of biopharmaceutics and particularly relates to a use of autologous peripheral blood stem cells combined with cytokines for improving low sperm activity. Inparticular, the combination of peripheral blood stem cells and cytokines of monocyte chemoattractant protein-1 (MCP-1) and glial cell-derived neurotrophic factors (GDNF) has a synergistic treatment oran inhibition function to improve the low sperm activity, and thus the new composition or a medicinal box for the low sperm activity is provided.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and specifically relates to the use of combination of stem cells and cytokines in improving sperm motility. Background technique [0002] Research into bone marrow transplantation was initiated after World War II in connection with the urgent need for treatment of massive radiation damage caused by atomic bombings. During the 1960s, bone marrow transplantation was used to treat leukemia, Hodgkin's disease and other diseases. But practice has shown that most transplants fail due to rejection. During the 1970s, the progress of HLA research brought a turning point to the pessimistic results of bone marrow transplantation, and the use of HLA-matched relatives' bone marrow transplantation to treat aplastic anemia and leukemia was also adopted. Platelet transfusion technology, new antibacterial A variety of coordinated measures such as drugs have ensured an increase in the success rate of bone marro...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61K38/18A61P15/08A61K35/28
CPCA61K35/28A61K38/195A61K38/185A61P15/08A61K2300/00
Inventor 芦福建刘玫
Owner SINO AMERICAN BIOTECH INC
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