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Method for in-situ remediation of cadmium-polluted soil by using microbial nanomaterials

A technology for cadmium-contaminated soil and nanomaterials is applied in the field of microbial nanomaterials for remediation of heavy metal cadmium-contaminated soil, which can solve the problems of high remediation conditions, long time, and the effect of soil treatment needs to be improved, and achieves convenient operation, large specific surface area, and solidification remediation. good effect

Active Publication Date: 2019-07-19
KETAI TECH CHENGDU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial remediation of cadmium-contaminated soil still needs to be improved.
For example, the Chinese patent application number CN201610370434.X introduces an in-situ and ex-situ coupling detoxification method for cadmium-contaminated sites based on biogas residues. It adds more auxiliary microbial culture materials to the soil, and the repair conditions are high. longer time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method of using microbial nanomaterials to repair cadmium-contaminated soil in situ, and its steps are:

[0026] A. Preparation of compound bacteria

[0027] The mass ratio of Desul foribrio sp., Desul fotomaculum sp., Desul fobacterium sp. and Desul fococcus sp. is 45:12:45:8. , Formulated into compound bacteria;

[0028] B. Cultivation of compound bacteria

[0029] The compound bacteria prepared in step A are anaerobic cultured with the sulfate-reducing bacteria culture in an anaerobic incubator to obtain a culture; the specific culture parameters are: temperature 37°C, pH7, and culture time 28 hours;

[0030] C. Preparation of suspending agent

[0031] The composite bacteria and their metabolites and nutrients obtained in step B culture are used as microbial nanomaterials, and water is added to prepare a microbial nanomaterial suspension. The number of bacteria in the microbial nanomaterial suspension is 1×10 7 Pcs / ml;

[0032] D. Remediation of cadmium contaminated soil

[00...

Embodiment 2

[0037] A method for repairing soil cadmium pollution in situ using microbial nanomaterials, and its steps are:

[0038] A. Preparation of compound bacteria

[0039] The mass ratio of Desul foribrio sp., Desul fotomaculum sp., Desul fobacterium sp. and Desul fococcus sp. is 55:15:30:7. , Formulated into compound bacteria;

[0040] B. Cultivation of compound bacteria

[0041] The compound bacteria prepared in step A are anaerobicly cultured with the sulfate-reducing bacteria culture in an anaerobic incubator to obtain a culture; the specific culture parameters are: temperature 44°C, pH 9.5, and culture time 40 hours;

[0042] C. Preparation of suspending agent

[0043] The composite bacteria and their metabolites and nutrients obtained from step B culture are used as microbial nanomaterials, and water is added to prepare a microbial nanomaterial suspension. The number of bacteria in the microbial nanomaterial suspension is 0.8×10 7 Pcs / ml;

[0044] D. Remediation of cadmium contaminated so...

Embodiment 3

[0050] A method for repairing soil cadmium pollution in situ using microbial nanomaterials, and its steps are:

[0051] A. Preparation of compound bacteria

[0052] The mass ratio of Desul foribrio sp., Desul fotomaculum sp., Desul fobacterium sp. and Desul fococcus sp. is 35:10:60:10. , Formulated into compound bacteria;

[0053] B. Cultivation of compound bacteria

[0054] The compound bacteria prepared in step A are anaerobic cultured with sulfate-reducing bacteria culture in an anaerobic incubator to obtain a culture; the specific culture parameters are: temperature 30°C, pH 4.5, and culture time 20 hours;

[0055] C. Preparation of suspending agent

[0056] The composite bacteria and their metabolites and nutrients obtained in step B culture are used as microbial nanomaterials, and water is added to prepare a microbial nanomaterial suspension. The number of bacteria in the microbial nanomaterial suspension is 1.2×10 7 Pcs / ml;

[0057] D. Remediation of cadmium contaminated soil

[00...

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Abstract

The invention relates to a method for in-situ remediation of cadmium-polluted soil by using microbial nanomaterials. The method mainly comprises the following steps: step A, the preparation of composite bacteria: preparing the composite bacteria from desulfovibrio, desulfotomaculum, desulfobacter and desulfococcus at a mas ratio of 35-55 to10-15 to 30-60 to 7-10; step B, culturing of composite bacteria: performing anaerobic culture on the composite bacteria with a sulfuric acid reducing bacteria culture; step C, preparation of a suspending agent: using the composite bacteria as well as metabolites and nutrients thereof as microbial nanomaterials, adding water to prepare a microbial nanomaterial suspending agent having the bacteria number of (0.8-1.2)*107 per ml; and step D, remediation ofcadmium-polluted soil: plowing the cadmium-polluted soil, simultaneously pouring the suspending agent according to a pouring amount of 50-500 L per square meter of soil, repeatedly plowing and pouringafter 7-15 days, wherein the total number of plowing and pouring times is 2-4 times. The method has advantages of no secondary pollution, short remediation time, convenient operation and good remediation effect.

Description

Technical field [0001] The invention relates to a method for repairing soil contaminated by heavy metal cadmium with microbial nano materials. [0002] technical background [0003] On April 17, 2014, the Ministry of Environmental Protection and the Ministry of Land and Resources jointly issued the "National Soil Pollution Status Survey Bulletin" that the excessive rate of heavy metal cadmium is as high as 7%. It is the primary pollutant among inorganic pollutants, and some agricultural dry land Excessive heavy metal cadmium. Cadmium in edible crops grown in soil contaminated by cadmium in dry land is often exceeded: in July 2017, the cadmium in wheat in Fengquan District, Xinxiang City, Henan Province exceeded the standard by 12.8 times. In 2017, the national standard "National Food Safety Standard Food Contaminants Limits" (GB 2762~2017) revised by the National Family Planning Commission and the Food and Drug Administration (GB 2762~2017) stipulates: the limit of cadmium in food...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 陈春坛李昕李卫张洪荣李福德
Owner KETAI TECH CHENGDU
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