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A method for in situ remediation of cadmium-contaminated soil using microbial nanomaterials

A technology of cadmium-contaminated soil and nanomaterials, which is applied in the field of microbial nanomaterials to restore heavy metal cadmium-contaminated soils. It can solve the problems of high repair conditions, long time, and soil treatment effects to be improved, and achieve convenient operation, large specific surface area, and solidification repair. good effect

Active Publication Date: 2020-12-22
KETAI TECH CHENGDU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial remediation of cadmium-contaminated soil still needs to be improved.
For example, the Chinese patent application number CN201610370434.X introduces an in-situ and ex-situ coupling detoxification method for cadmium-contaminated sites based on biogas residues. It adds more auxiliary microbial culture materials to the soil, and the repair conditions are high. longer time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for in-situ remediation of cadmium-contaminated soil using microbial nanomaterials, the steps of which are as follows:

[0026] A. Preparation of complex bacteria

[0027] The Desulfovibrio (Desul foribrio sp.), Desulfur enterobacteria (Desul fotomaculum sp.), Desulfobacterium (Desul fobacterium sp.) and Desulfococcus (Desul fococcus sp.) in the mass ratio of 45:12:45:8 , formulated into composite bacteria;

[0028] B. Cultivation of complex bacteria

[0029] The composite bacteria prepared in step A are anaerobically cultured in an anaerobic incubator with cultures of sulfate-reducing bacteria to obtain cultures; the specific culture parameters are: temperature 37°C, pH 7, culture time 28 hours;

[0030] C. Preparation of suspension

[0031] The compound bacteria and its metabolites and nutrients obtained in the B step culture are used as microbial nanomaterials together, and water is added to prepare microbial nanomaterial suspensions. The number of bacter...

Embodiment 2

[0037] A method for in-situ remediation of soil cadmium pollution using microbial nanomaterials, the steps of which are as follows:

[0038] A. Preparation of complex bacteria

[0039] Desulfovibrio (Desul foribrio sp.), Desulfoenteric bacteria (Desul fotomaculum sp.), Desulfobacterium (Desul fobacterium sp.) and Desulfococcus (Desul fococcus sp.) were mixed according to the mass ratio of 55:15:30:7 , formulated into composite bacteria;

[0040] B. Cultivation of complex bacteria

[0041] The composite bacteria prepared in step A are anaerobically cultured in an anaerobic incubator with cultures of sulfate-reducing bacteria to obtain cultures; the specific culture parameters are: temperature 44°C, pH 9.5, culture time 40 hours;

[0042] C. Preparation of suspension

[0043] The compound bacteria and its metabolites and nutrients obtained in the B step culture are used as microbial nanomaterials together, and water is added to prepare microbial nanomaterial suspensions. The ...

Embodiment 3

[0050] A method for in-situ remediation of soil cadmium pollution using microbial nanomaterials, the steps of which are as follows:

[0051] A. Preparation of complex bacteria

[0052] Desulfovibrio (Desul foribrio sp.), Desulfur enterobacteria (Desul fotomaculum sp.), Desulfobacillus (Desul fobacterium sp.) and Desulfococcus sp. (Desul fococcus sp.) according to the mass ratio of 35:10:60:10 , formulated into composite bacteria;

[0053] B. Cultivation of complex bacteria

[0054] The composite bacteria prepared in step A are anaerobically cultured in an anaerobic incubator with cultures of sulfate-reducing bacteria to obtain cultures; the specific culture parameters are: temperature 30°C, pH 4.5, culture time 20 hours;

[0055] C. Preparation of suspension

[0056] The composite bacteria obtained in the B step culture and its metabolites and nutrients are used as microbial nanomaterials together, and water is added to prepare microbial nanomaterial suspensions. The number...

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Abstract

The invention relates to a method for in-situ remediation of cadmium-polluted soil by using microbial nanomaterials. The method mainly comprises the following steps: step A, the preparation of composite bacteria: preparing the composite bacteria from desulfovibrio, desulfotomaculum, desulfobacter and desulfococcus at a mas ratio of 35-55 to10-15 to 30-60 to 7-10; step B, culturing of composite bacteria: performing anaerobic culture on the composite bacteria with a sulfuric acid reducing bacteria culture; step C, preparation of a suspending agent: using the composite bacteria as well as metabolites and nutrients thereof as microbial nanomaterials, adding water to prepare a microbial nanomaterial suspending agent having the bacteria number of (0.8-1.2)*107 per ml; and step D, remediation ofcadmium-polluted soil: plowing the cadmium-polluted soil, simultaneously pouring the suspending agent according to a pouring amount of 50-500 L per square meter of soil, repeatedly plowing and pouringafter 7-15 days, wherein the total number of plowing and pouring times is 2-4 times. The method has advantages of no secondary pollution, short remediation time, convenient operation and good remediation effect.

Description

technical field [0001] The invention relates to a method for remediating soil polluted by heavy metal cadmium with microbial nanomaterials. [0002] technical background [0003] On April 17, 2014, the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources announced that the excessive rate of heavy metal cadmium was as high as 7%. There is excessive heavy metal cadmium. Cadmium levels in edible crops grown in cadmium-contaminated soils in drylands are common: In July 2017, cadmium levels in wheat in Fengquan District, Xinxiang City, Henan Province exceeded standards by 12.8 times. In 2017, the national standard "Food Safety National Standards Contaminant Limits in Food" (GB 2762-2017) revised by the State Family Planning Commission and the State Food and Drug Administration stipulated that: cadmium limit indicators in food: cadmium in grains 0.1mg / kg; fresh vegetables ( Leafy vegetables) 0....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 陈春坛李昕李卫张洪荣李福德
Owner KETAI TECH CHENGDU
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