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Growth-related SNPs in Largemouth Bass and Their Applications

A largemouth bass, growth speed technology, applied in the field of SNP loci related to the growth traits of largemouth bass, can solve the problems of large growth disparity, mutual cannibalism, and the quality of largemouth bass parents can not reach, and achieve fast growth , the effect is direct, and the effect of reducing blindness

Active Publication Date: 2022-07-19
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is convenient, fast, and simple, but it is greatly affected by human factors. In addition, largemouth bass is a carnivorous fish with strong predatory nature. If the bait is insufficient, it will eat each other, resulting in a large difference in growth.
It can be seen that judging the parental quality of largemouth bass only from phenotype often does not achieve the desired effect

Method used

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  • Growth-related SNPs in Largemouth Bass and Their Applications
  • Growth-related SNPs in Largemouth Bass and Their Applications
  • Growth-related SNPs in Largemouth Bass and Their Applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Acquisition of SNP markers associated with largemouth bass growth traits

[0047] The study found in this application that the amino acid sequence of the largemouth bass heat shock protein HSC70-1 (heat shock cognate protein 70) is shown in SEQ ID NO: 1, and the full length of the coding region sequence of the largemouth bass HSC70-1 gene is 3382 bp (SEQ ID NO: 1). NO: 2), consisting of 8 exons and 7 introns. A SNP site was found at positions 821 and 2804 of the largemouth bass heat shock protein HSC70-1 gene sequence (SEQ ID NO: 2), respectively, and the alleles C and Allele G constitutes three genotypes CC, GC and GG; there are allele A and allele T at the 2804th base position, constituting three genotypes AA, AT and TT.

[0048] The random population of 430 samples used for the association analysis in this experiment was bred in the same batch and reared in the same pool, and the sampling time was the same. Therefore, the differences in time, environment a...

Embodiment 2

[0061] Embodiment 2 A kind of method of screening fast-growing largemouth bass

[0062] Utilize the above-mentioned SNP locus to screen the fast-growing largemouth bass parent, including the following steps:

[0063] (1) Primer sequence:

[0064] Two pairs of primers were designed according to the gene sequence of largemouth bass heat shock protein HSC70-1 for PCR amplification. The designed and synthesized primers are as follows:

[0065] P1F: 5'-TGAAGCCTACCTCGGAAAAGT-3' (SEQ ID NO. 3),

[0066] P1R: 5'-AGCATCCTTAGTGGCCTGGCGC-3' (SEQ ID NO. 4),

[0067] P2F: 5'-TCCACCAGCTTATCAGACTGT-3' (SEQ ID NO. 5),

[0068] P2R: 5'-GGTCAACCCTCCAAGTAACTT-3' (SEQ ID NO. 6);

[0069] The primers were expected to amplify 2 DNA bands, SEQ ID NO: 9 (176 bp in size) and SEQ ID NO: 10 (222 bp in size).

[0070] (2) Sample DNA submission (alkaline lysis method):

[0071] 1. Cut 10-20 mg of largemouth bass fin rays to be tested and put them into a clean EP tube;

[0072] 2. Add 180 µL of 50 m...

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Abstract

The invention discloses a growth-related SNP site of largemouth bass and its application. The growth-related SNP site is located at the 821st and 2804th bases of the HSC70-1 gene sequence. The present invention finds that there are allele C and allele G at the C+821G position of HSC70-1, forming three genotypes CC, GC and GG, and there are allele A and allele T at the A+2804T position, Three genotypes AA, AT and TT are composed. The growth traits of individuals with CC and TT genotypes at bases 821 and 2804 were significantly better than those of other genotypes. Using this phenomenon, largemouth bass parents with CC and TT genotypes retained in production and individuals of other genotypes are removed, and largemouth bass varieties with fast growth speed and stable genetics can be quickly obtained, that is, the SNP site of the present invention can be applied to Screening of fast-growing largemouth bass parents.

Description

technical field [0001] The invention belongs to the field of molecular biology technology, and particularly relates to SNP sites associated with growth traits of largemouth bass and applications thereof. Background technique [0002] largemouth bass ( Micropterus salmoides L.), also known as California perch, native to North America, has the advantages of strong adaptability, fast growth, less disease, low temperature resistance, short growth cycle and delicious taste, and is one of the important freshwater aquaculture fish. In 1983, it was introduced from Taiwan, China to Guangdong Province, and it is now cultivated in most parts of the country. However, over the past 30 years since the introduction, the production units did not pay attention to the operating procedures to be followed by the parents, nor did they regularly replenish and introduce the parents from the place of origin, resulting in a decrease in the genetic diversity of the farmed largemouth bass, which in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46
CPCC07K14/461C12Q1/6888
Inventor 李胜杰樊佳佳姜鹏白俊杰孙建国吴建开
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI