Suspension of Pichia japonica for controlling postharvest disease of cherry tomato fruit

A technology for cherry tomato and postharvest diseases, applied in the directions of yeast-containing food ingredients, microorganism-based methods, applications, etc., can solve the serious harm to human health and environmental safety of chemical fungicides, the resistance of pathogenic microorganisms, and affect the effect of disease prevention and control. and other problems to achieve the effect of reducing the incidence of Botrytis cinerea, improving the direct inhibitory effect and cost-effective

Active Publication Date: 2022-07-26
ZHEJIANG UNIV
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AI Technical Summary

Problems solved by technology

[0004] Residues of chemical fungicides are likely to cause serious harm to human health and environmental safety, and at the same time lead to drug resistance of pathogenic microorganisms, affecting the effect of disease control

Method used

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  • Suspension of Pichia japonica for controlling postharvest disease of cherry tomato fruit
  • Suspension of Pichia japonica for controlling postharvest disease of cherry tomato fruit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the screening of Pichia quaternary yeast yeast:

[0027] Select the sea sand of the Sansha Islands in Hainan Province;

[0028] The culture reagent is Tiger Red (Red Bengal) medium;

[0029] The culture screening conditions are: weigh 10 g of sea sand, add 100 mL of sterile water, shake at 200 r / min for 30 min, and then absorb the supernatant, and then dilute the supernatant gradient with sterile water for 10 and 10 minutes. 2 , 10 3 , 10 4 100 μL were drawn from each gradient and spread evenly on the tiger red medium plate, cultured in the incubator at 28°C for 3 days, selected according to the colony morphology and streaked and purified, and confirmed as yeast by microscopy and PCR amplification by 16s rDNA sequencing. After that, it was stored in the slant of NYDA medium.

[0030] Finally, the strain J-SS-CX was screened out, which was identified by 16s rDNA as Meyerozyma guilliermondii, and its colony characteristics were as follows: round, milky w...

Embodiment 2

[0035] Embodiment 2, the preparation method of Pichia quaternary yeast suspension:

[0036]Pichia quaternary yeast J-SS-CX (CGMCC NO.17240) was stored in NYDA medium at low temperature (4°C), taken out during activation and cultured in NYDA medium at 28°C for 48 hours, and subcultured repeatedly. After 2 times, the activated Pichia moniliformis was added to the NYDB medium with an inoculation loop, cultivated at 200rpm and 28°C for 24h, and then sucked 1 / 50 volume of the culture medium to contain 12% NaCl. In NYDB medium (i.e., bacterial liquid: NYDB medium containing 12% NaCl = 1:50 volume ratio), subculture at 200 rpm and 28 °C for 24 h, collect the culture medium, and at 4000 rpm and 4 °C under the conditions After centrifugation for 15 min, the cells collected by centrifugation were washed twice with sterile distilled water to remove the culture medium. Dilute and resuspend the yeast cells with sterile distilled water, use a hemocytometer to determine the concentration un...

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Abstract

The invention discloses a kind of Pichia quaternary yeast J-SS-CX, which is Meyerozyma guilliermondii, and the deposit number is CGMCC NO.17240. The invention also discloses a Pichia moniliformis suspension for controlling postharvest diseases of cherry tomato fruits. The preparation method is as follows: Pichia moniliformes J-SS activated in NYDA solid medium ‑CX was inoculated into NYDB medium for one generation, and then inoculated into NYDB medium containing 12% NaCl for culture, and the obtained cells were washed and diluted with sterile distilled water. The invention can realize effective control of postharvest diseases of fruits without using chemical fungicides, and the yeast preparation is convenient to operate, has good effect, low cost, safety and environment-friendly.

Description

technical field [0001] The invention relates to the technical field of post-harvest disease prevention and control of fruits, in particular to a biological fresh-keeping technology for inhibiting post-harvest diseases of fruits through direct action. Background technique [0002] Affected by post-harvest diseases, logistics technology and a variety of abiotic factors, the post-harvest losses of fruits are relatively serious. Among them, the infection of pathogenic fungi is the main cause of postharvest rot of fruits. The common pathogenic fungi are Penicillum spp., Botrytis spp. and Alternaria spp. .)Wait. According to statistics, nearly 80 million tons of fruits in my country are affected by post-harvest diseases and cannot be sold each year, and the economic losses amount to 100 billion yuan, which seriously restricts the development of my country's fruit industry. [0003] At present, chemical fungicides are still the main measures to control postharvest diseases of fru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23B7/155C12R1/84
CPCA23B7/155C12N1/16A23V2002/00C12R2001/84C12N1/165A23V2200/10A23V2250/76
Inventor 郑晓冬曹璇
Owner ZHEJIANG UNIV
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