Application of dihydromyricetin in preparing products for preventing or treating obesity
A technology for dihydromyricetin and obesity, which is applied in the field of nutrition to achieve the effects of no drug resistance, no toxic side effects, and significant preventive and therapeutic effects
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Embodiment 1 2
[0054] Example 1 Dihydromyricetin prevents weight gain in mice
[0055] 1. Experimental method
[0056] In order to explore the role of dihydromyricetin in preventing weight gain in mice, the mice from 4 to 12 weeks old were fed a high-fat diet every day; at the same time, the mice in the experimental group were fed with 200 mg / kg dihydromyricetin every morning, The mice in the control group were fed with an equal volume of PBS, and the mice in the experimental group and the control group were fed with no more than 200 μL each time. After continuous feeding for 8 weeks, the changes in the body weight of the mice were observed.
[0057] 2. Results
[0058] After feeding with dihydromyricetin and PBS respectively from the age of 4 weeks to the age of 12 weeks, the comparison chart of the changes in the body weight of the mice is shown in figure 1 As shown, the comparison chart of the feed intake of mice is shown in figure 2 As shown, it can be seen that on the basis of not i...
Embodiment 2
[0059] Embodiment 2 mouse nuclear magnetic resonance imaging
[0060] 1. Experimental method
[0061] The mice in the experimental group (fed with dihydromyricetin from the age of 4 weeks to 12 weeks) and the mice in the control group (fed with PBS from the age of 4 weeks to 12 weeks) were placed in the MRI apparatus respectively, Statistical determination of the respective muscle content and fat content ratio, the result analysis.
[0062] 2. Results
[0063] image 3 It is a comparison chart of muscle content and fat content of nuclear magnetic mice, Figure 4 It is a comparison chart of visceral fat, subcutaneous fat and liver tissue weight in mice, Figure 5 is a life-size comparison of visceral fat, subcutaneous fat, and liver tissue in mice, Figure 3-5 The results showed that dihydromyricetin can significantly reduce the muscle content of the body, reduce the size and fat content of various fat-related tissues, inhibit the fat accumulation induced by high-fat diet,...
Embodiment 3
[0064] Example 3 HE staining of mouse visceral fat, subcutaneous fat and liver tissue sections
[0065] (1) Paraffin sections are routinely dewaxed with xylene and ethanol to water;
[0066] (2) Hematoxylin staining for 10 minutes;
[0067] (3) Rinse with running water, remove residual color, differentiate in 0.7% hydrochloric acid ethanol for a few seconds, rinse with running water, the slices turn blue for about 15 minutes, and 95% ethanol for 30 seconds;
[0068] (4) Alcoholic eosin staining for 30 seconds;
[0069] (5) Rinse with distilled water for 30 seconds;
[0070] (6) I 95% ethanol for 30 seconds, II 95% ethanol for 30 seconds, I 100% ethanol for 30 seconds, II 100% ethanol for 30 seconds;
[0071] (7) Phenolic xylene for 30 seconds, (1:4 phenolic 1-xylene 4), I xylene for 30 seconds, II xylene for 30 seconds;
[0072] (8) Seal the slides with neutral gum and take pictures.
[0073] 2. Results
[0074] Image 6 It is the HE staining results of visceral fat, su...
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