A 3D printed microfluidic device and its large-throughput method for preparing monodisperse emulsions

A 3D printing and microfluidic technology, applied in the field of microfluidics, can solve the problems of microfluidic chip preparation of droplets in small quantities, rough structure of microfluidic chips, and difficult industrial production, etc., and achieves excellent monodispersity, Simple structure and easy operation

Active Publication Date: 2021-06-01
绍兴钠钇光电有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The microfluidic chips used today are less able to prepare a large number of droplets, the structure is complex, and it is difficult to realize industrial production. The ladder emulsification structure is relatively simple, but the structure of the microfluidic chip generated by the common method is relatively rough, and the generated droplets are larger. Therefore, the application of 3D printers to the production of microfluidic chips can avoid the above shortcomings, and can make the entire detection integration miniaturized and automated, with high throughput, low consumption of detection reagents, small sample volume requirements, and less pollution. Capable of rapid mass production of microfluidic chips with finer structures

Method used

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  • A 3D printed microfluidic device and its large-throughput method for preparing monodisperse emulsions
  • A 3D printed microfluidic device and its large-throughput method for preparing monodisperse emulsions
  • A 3D printed microfluidic device and its large-throughput method for preparing monodisperse emulsions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The channels and slits are printed as a whole to form a sheet-like rectangular structure. There are two rows and 6 channels. The dispersed phase distribution device is a thicker shell structure, and then the two are combined with AB glue. The dispersed phase is injected using a syringe pump. The obtained microfluidic devices are as figure 1 shown. The dispersed phase used is water, the total pipe flow rate is 30ml / h, and the continuous phase is dimethyl oil bath silicone oil. Since the viscosity of silicone oil is 50cs. The syringe pump uses the laboratory micro-syringe pump LSP01-1A of LongerPump Company. Dowcorning 749 was added as a surfactant in the oil phase with a mass concentration of 5 wt%, and a monodisperse emulsion with uniform and controllable size could be obtained.

Embodiment 2

[0039]The shape of the device is circular pie, and there is a cylindrical liquid storage tank with a diameter of 24mm and a height of 3mm inside. The device has 12 channels evenly arranged, the central angle between each channel is 30°, and the slit is on the side of the device. Microfluidic devices such as figure 2 As shown, the specific structural parameters are as Figure 4 shown. The dispersed phase used is water, the total pipe flow rate is 30ml / h, and the continuous phase is dimethyl oil bath silicone oil. Since the viscosity of silicone oil is 50cs. The syringe pump uses the laboratory micro-syringe pump LSP01-1A of LongerPump Company. Dowcorning 749 was added as a surfactant in the oil phase, and its mass concentration was 5wt%. Under the above conditions, this microfluidic device was used for experiments, and the device produced continuous droplets with uniform sizes, and more than 90% of the droplets were distributed in a diameter of 0.8-1.2 m and uniform in si...

Embodiment 3

[0041] The shape of the device is circular pie, and there is a cylindrical liquid storage tank with a diameter of 24mm and a height of 3mm inside. This device has 6 channels evenly arranged, and the central angle between each channel is 60°. The slit is on the side of the device, and the corresponding inclination angle is set in the slit area, that is, the top and bottom walls of the slit An inclination angle of α is opened from the connecting channel to the outside to control the size of the generated droplets. Microfluidic devices such as image 3 shown. The dispersed phase used is water, the total pipe flow rate is 30ml / h, and the continuous phase is dimethyl oil bath silicone oil. Since the viscosity of silicone oil is 50cs. The syringe pump uses the laboratory micro syringe pump LSP01-1A of LongerPump Company. Dowcorning 749 was added as a surfactant in the oil phase, and its mass concentration was 5wt%. Under the above conditions, the microfluidic device is used for...

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Abstract

The invention provides a 3D printing microfluidic device and a method for preparing a monodisperse emulsion with large throughput. 3D printing is used to make microfluidic devices. The device is in the shape of a cylinder with a cylindrical channel in the center. The liquid buffer channel is connected to multiple radially distributed microchannels. The microchannels are connected to the external collection tank through the slit area. The slit The area is flat and wedge-shaped. When the dispersed phase is injected from the liquid inlet, enters the liquid buffer channel, flows into the radially distributed microchannels from the liquid buffer channel, and when the fluid enters the flat slit area through the microchannel, under the extrusion of the side of the slit, it has a relatively Large Laplace pressure, the wedge-shaped structure will cause the droplet to be subjected to a Laplace pressure difference between the inside and outside, the pressure difference pushes the droplet to flow in the wedge-shaped structure, and finally squeezes the droplet from the wedge-shaped structure to the collection tank middle. The size of the generated droplets can be controlled by changing the structural parameters of the channel, and the size of the prepared droplets is uniform, so that a large number of monodisperse emulsions with uniform and controllable sizes can be prepared. The method has the advantages of simple manufacture and low cost, and can realize industrialized production.

Description

technical field [0001] The invention provides a three-dimensional microfluidic device designed and manufactured based on 3D printing technology, and a large flux of monodisperse emulsion is prepared by a step emulsification method. It belongs to the field of microfluidic technology. Background technique [0002] Microfluidic technology integrates the functions of biochemical laboratories into a chip with a size of a few centimeters, which can effectively realize the miniaturization, automation, integration and portability of various functions such as analysis, detection and reaction. Now it has become a new research field interdisciplinary of biology, chemistry, medicine, electronics, machinery and materials. [0003] The microfluidic chip is an important platform for the realization of microfluidic technology. The main feature of the microfluidic chip is its internal microchannels, and the structural dimensions of the reaction chamber and capture chamber are all on the mic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502784
Inventor 何赛灵陈东王行政陈飞鸿
Owner 绍兴钠钇光电有限公司
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