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Antioxidant peony peptide and preparation method thereof

A peony peptide and anti-oxidation technology, which is applied in the production of peptides, anti-toxic agents, bulk chemicals, etc., can solve the problem of low antioxidant capacity of peony peptide and achieve good anti-oxidation effect

Pending Publication Date: 2019-08-09
世堃堂(广东)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the currently obtained peony peptides only rely on the antioxidant capacity of amino acid residues, so the antioxidant capacity of the obtained peony peptides is not high and needs to be improved

Method used

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  • Antioxidant peony peptide and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] An antioxidant peony peptide, the peony peptide includes 6-9 amino acids, including one or more tyrosine, cystine, and methionine.

[0026] A preparation method of the antioxidant peony peptide, comprising the following steps:

[0027] S1: Extracting peony protein from peony seed meal;

[0028] S2: extracting the peony protein obtained in step S1 with supercritical carbon dioxide;

[0029] S3: Take the peony protein in step S2, add distilled water to dissolve the peony protein according to the mass ratio of 1:20, adjust the temperature at 37°C, and adjust the pH of the solution to 8;

[0030] S4: Add 2% of the amount of peony protein to the solution in step S3, a ratio of 1:2 of ficin and subtilisin, and the enzymatic hydrolysis time is 30 minutes;

[0031] S5: heating the solution after the enzymatic hydrolysis in step S4 to 100°C, keeping it for 10 minutes, and then returning to 50°C;

[0032] S6: add 6% of peony protein in step S5, bromelain, papain and endopeptid...

Embodiment 2

[0036] An antioxidant peony peptide, the peony peptide includes 6-9 amino acids, including one or more tyrosine, cystine, and methionine.

[0037] A preparation method of the antioxidant peony peptide, comprising the following steps:

[0038] S1: Extracting peony protein from peony seed meal;

[0039] S2: extracting the peony protein obtained in step S1 with supercritical carbon dioxide;

[0040] S3: Take the peony protein in step S2, add distilled water to dissolve the peony protein according to the mass ratio of 1:25, adjust the temperature at 40°C, and adjust the pH of the solution to 10;

[0041] S4: 3% of the amount of peony protein is added to the solution in step S3, and the ratio is 1:2.5 of ficin and subtilisin, and the enzymatic hydrolysis time is 50 minutes;

[0042] S5: The temperature of the solution after the enzymatic hydrolysis in step S4 is raised to 100° C., kept for 15 minutes, and then returned to 55° C.;

[0043] S6: Add 6% of the peony protein content ...

Embodiment 3

[0047] An antioxidant peony peptide, the peony peptide includes 6-9 amino acids, including one or more tyrosine, cystine, and methionine.

[0048] A preparation method of the antioxidant peony peptide, comprising the following steps:

[0049] S1: Extracting peony protein from peony seed meal;

[0050] S2: extracting the peony protein obtained in step S1 with supercritical carbon dioxide;

[0051] S3: Take the peony protein in step S2, add distilled water to dissolve the peony protein according to the mass ratio of 1:22, adjust the temperature at 39°C, and adjust the pH of the solution to 10;

[0052] S4: adding 2.5% of the amount of peony protein to the solution in step S3, ficin and subtilisin in a ratio of 1:2, and the enzymatic hydrolysis time is 40 minutes;

[0053] S5: heating the solution after the enzymatic hydrolysis in step S4 to 100°C, keeping it for 10 minutes, and then returning to 50°C;

[0054] S6: Add 7% of the peony protein content in step S5, bromelain, pap...

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Abstract

The invention relates to the field of plant extracts, in particular to an antioxidant peony peptide and a preparation method thereof. The peony peptide comprises 6-9 amino acids, and comprises one ormore of tyrosine, cystine and methionine. According to the preparation method, a two-step decomposition method is utilized, ficin proteinase with not high specificity and subtilisin are selected and compounded, firstly, peptide bonds at main glutamic acids in peony seed meal protein are ruptured, and a protein peptide fragment is formed; then a small amount of bromelain without specificity, papainwith specificity and endopeptidase Glu-C with specificity are utilized, and the tyrosine, the cystine and the methionine are retained in peptide chain molecules as much as possible to improve the antioxidant capacity of the protein peptide.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to an antioxidant peony peptide and a preparation method thereof. [0002] technical background [0003] Peony is a plant of Ranunculaceae and Paeoniae genus, known as "National Beauty and Heavenly Fragrance". Peony seed oil developed from peony seeds is a natural pollution-free food integrating nutrition and health care. The protein content of peony seed meal, one of the by-products of pressing peony seed oil, reaches 19.03%. [0004] Protein is an important component of food. Protein peptides are obtained after enzymatic hydrolysis. Its functional and biological characteristics are quite different from the original protein. For example, the solubility, emulsification and foaming of protein can be improved after enzymatic hydrolysis. There are a large number of peony seed meal products after oil refining in my country every year, and a large amount of peony protein can be obtained. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06A61P39/06
CPCA61P39/06C07K7/06C12P21/06Y02P20/54
Inventor 王世英赵欣谢瑞生
Owner 世堃堂(广东)生物科技有限公司
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