Method of extracting myoglobin from beef

A myoglobin and extraction method technology, applied in hemoglobin/myoglobin, chemical instruments and methods, specific peptides, etc., can solve the problems of unfavorable sample storage application, difficult myoglobin, easy oxidation of myoglobin, etc. Achieve the effect of maintaining reduction and biological activity, improving purity and avoiding oxidation
CN110105445AInactive Publication Date: 2019-08-09甘肃省农业科学院农产品贮藏加工研究所
0 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
甘肃省农业科学院农产品贮藏加工研究所
Publication Date
2019-08-09
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a method of extracting myoglobin from beef. The method includes the steps of adding a Tris-HCl mixed buffer solution to a beef meat sample, homogenizing, standing, centrifuging,adding (NH4)2SO4 and standing, centrifuging, adding a Tris-HCl buffer solution b, performing dialysis, and eluting with a NaCl mixed solution in a chromatography column to obtain the myoglobin in thebeef. According to the method, the pH value of the Tris-HCl mixed buffer solution, the extraction time and the saturation of the myoglobin salt precipitation solution are optimized to increase the extraction amount of myoglobin; the Tris-HCl mixed buffer solution contains glycerin and dithiothreitol, the glycerin maintains the structural stability of the myoglobin and facilitates rapid precipitation, and the dithiothreitol maintains the reductive and biological activity of the myoglobin; the solid (NH4)2SO4 is added for fractional salt precipitation to increase the purity of the myoglobin, the temperature is kept at 4 DEG C to prevent degeneration and oxidation of the myoglobin, residual salt ions and other small molecular proteins can be effectively removed through the dialysis to improve the purity of the myoglobin, and the separation by gel filtration chromatography carried out at last does not destroy the structure and function of the myoglobin.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of protein extraction, and in particular relates to a method for extracting myoglobin in beef. Background technique

[0002] Among the extraction and purification methods of beef myoglobin, the Tris-HCl method is commonly used for crude myoglobin extraction, but the existing myoglobin extraction technology has complex muscle tissue components, and it is difficult for myoglobin to precipitate quickly and stably; Moreover, (oxygenated) myoglobin itself is easily oxidized, which is not conducive to the preservation and application of purified samples. It is necessary to improve the stability of myoglobin during the purification process and reduce the oxidation and denaturation loss of myoglobin. Contents of the invention

[0003] The technical problem to be solved by the present invention is to provide a method for extracting myoglobin in beef for the above-mentioned deficiencies in the prior art. The satura...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More