Compound ulva extract with auxiliary anti-tumor effect and preparation technology of compound ulva extract

A technology of extract and anti-tumor activity, which is applied in the field of medicine to achieve the effects of small side effects, inhibition of growth and spread, and definite killing ability

Inactive Publication Date: 2019-08-09
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, the method for preparing Ulva polysaccharide from green algae by oxid

Method used

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  • Compound ulva extract with auxiliary anti-tumor effect and preparation technology of compound ulva extract
  • Compound ulva extract with auxiliary anti-tumor effect and preparation technology of compound ulva extract
  • Compound ulva extract with auxiliary anti-tumor effect and preparation technology of compound ulva extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Ulva is soaked and desalted by conventional methods, dried and crushed, and 60% v / v ethanol is added to Ulva dry powder for reflux extraction for 1 hour. The ratio of dry powder weight g to ethanol volume mL is 1:20, and then dried after reflux Dried to obtain dried degreased Ulva powder, reclaim ethanol for recycling;

[0027] (2) Add distilled water to the degreased Ulva powder prepared in step (1), wherein the ratio of Ulva mass g to distilled water volume mL is 1:30, and at an extraction temperature of 60° C., assisted extraction with 350W ultrasonic waves for 120 minutes, Then leaching in hot water at 80°C for 2 hours, after the extraction, filter, repeat the extraction of the filter residue twice, combine the filtrate, centrifuge at 4000rpm for 10 minutes, and concentrate the supernatant under reduced pressure at 65°C to the original volume Add 95% v / v ethanol to make the ethanol concentration in the system reach 80% v / v, then let it stand at 4°C for 8 hours, ...

Embodiment 2

[0037] (1) Ulva is soaked and desalted by conventional methods, dried and pulverized, and 70% v / v ethanol is added to Ulva dry powder for reflux extraction for 2 hours. The ratio of dry powder weight g to ethanol volume mL is 1:25, and then dried after reflux Dried to obtain dried degreased Ulva powder, reclaim ethanol for recycling;

[0038] (2) Add distilled water to the degreased Ulva powder prepared in step (1), wherein the ratio of the mass g of Ulva to the volume mL of distilled water is 1:35, and extract with 400W ultrasonic wave for 120 minutes at an extraction temperature of 65°C, Then leaching in hot water at 85°C for 3 hours, after the extraction, filter, repeat the extraction of the filter residue 3 times, combine the filtrate, centrifuge at 4500rpm for 15 minutes, and concentrate the supernatant under reduced pressure at 65°C to the original volume Add 95% v / v ethanol to make the ethanol concentration in the system reach 80% v / v, then let it stand at 4°C for 12 ho...

Embodiment 3

[0048] (1) Ulva is soaked and desalted by conventional methods, dried and crushed, and 80% v / v ethanol is added to Ulva dry powder for reflux extraction for 2 hours. The ratio of dry powder weight g to ethanol volume mL is 1:30, and then dried after reflux Dried to obtain dried degreased Ulva powder, reclaim ethanol for recycling;

[0049] (2) Add distilled water to the degreased Ulva powder prepared in step (1), wherein the ratio of the mass g of Ulva to the volume mL of distilled water is 1:40, and extract with 400W ultrasonic wave for 120 minutes at an extraction temperature of 70°C, Then leaching in hot water at 90°C for 3 hours, after the extraction, filter, repeat the extraction of the filter residue 3 times, combine the filtrate, centrifuge at 5000rpm for 20 minutes, and concentrate the supernatant under reduced pressure at 70°C to the original volume Add 95% v / v ethanol to make the ethanol concentration in the system reach 80% v / v, then let it stand at 4°C for 12 hours...

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Abstract

The invention discloses a preparation technology of a compound ulva extract with an auxiliary anti-tumor effect. The process is characterized in that ultrasonic-assisted extraction is utilized, crudepolysaccharide ingredients are separated by adopting a water-extraction and alcohol-precipitation method, then deproteinization and depigmentation are carried out, ulva active polysaccharide with highpurity is obtained after purification, then hydrogen peroxide and ascorbic acid are used for oxidizing and degrading the ulva polysaccharide, ulva active oligosaccharide with high purity is obtainedafter purification, and the ulva polysaccharide and the ulva oligosaccharide are mixed for use. Through the verification of animal pharmacodynamics, the compound ulva extract has an exact killing ability against an H22 hepatocarcinoma tumor in KM mice, can inhibit the growth and spread of the tumor, has a certain repair effect on the diseased tissue, and does no have obvious side effects. The compound ulva extract is a biologically active substance which has no toxicity, has few side effects and is very safe, and can be conveniently prepared into drugs of various dosage forms with pharmaceutically acceptable carriers.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a preparation technology of compound Ulva extract with auxiliary antitumor effect. Background technique [0002] As a malignant disease, tumor has a high fatality rate, and patients are also troubled by its accompanying pain, and its prevalence is increasing year by year, and the society's demand for anti-tumor drugs is increasing year by year. Traditionally used anti-tumor drugs often have various side effects or are very expensive, so there is a considerable demand for new anti-tumor drugs with high efficiency, low side effects, low cost and wide applicability. Many of the natural high molecular compounds in seaweed, such as polysaccharides, have certain anti-tumor effects, but because polysaccharides cannot be directly absorbed, they act on the intestinal flora, while oligosaccharides, as polysaccharide degradation products, can be directly absorbed by the human bo...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00
CPCA61K31/715A61P35/00C08B37/0003C08B37/006
Inventor 赵超吴德胜林国鹏刘丹肖晓玮
Owner FUJIAN AGRI & FORESTRY UNIV
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