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Fluorescent nanometer standard plate, preparation method thereof and application

A fluorescent nanometer, standard plate technology, applied in fluorescence/phosphorescence, color/spectral property measurement, material analysis by optical means, etc. Contrasting, easy-to-use effects

Pending Publication Date: 2019-08-09
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of the prior art that the fluorescent substance on the fluorescent nano scale is fixed, the secondary use operation is complicated, and the flexibility is low, thereby disclosing a fluorescent nano standard plate and the fluorescent nano scale Preparation method and application of standard plate

Method used

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  • Fluorescent nanometer standard plate, preparation method thereof and application
  • Fluorescent nanometer standard plate, preparation method thereof and application
  • Fluorescent nanometer standard plate, preparation method thereof and application

Examples

Experimental program
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Effect test

Embodiment 1

[0046] This embodiment provides a specific implementation of a fluorescent nanometer standard plate.

[0047] A fluorescent nanostandard plate, such as figure 1 As shown, it includes a nanoscale structure 1 and a flow cell, and the flow cell includes a flow chamber 2, and the flow chamber 2 is arranged on a side of the nanoscale structure 1 having a nanowire structure, and the flow chamber 2 is connected to the nanoscale structure. Line structures meet. Combining the flow cell with the nanoscale structure 1 can not only realize the fluorescence of the nanoscale structure 1, but also realize the rapid replacement of various fluorescent substances through the flow cell; in other embodiments, the flow cell can be an opening For a flow cell, a closed flow cell or a microchannel flow cell, the nanometer scale structure 1 can be used repeatedly, and the operation is simple, fast, efficient, high in flexibility and accurate in test results in actual use.

[0048] In this embodiment, ...

Embodiment 2

[0064] This embodiment provides a specific implementation of a fluorescent nanometer standard plate.

[0065] A fluorescent nanostandard plate, such as Figure 4 As shown, it includes a nanoscale structure 1 and a flow cell, and the flow cell includes a flow chamber 2, and the flow chamber 2 is arranged on a side of the nanoscale structure 1 having a nanowire structure, and the flow chamber 2 is connected to the nanoscale structure. Line structures meet.

[0066] The nano-scale structure 1 includes a nano-groove mask structure 12 and a substrate 11 carrying the nano-groove mask structure, and the substrate 11 is transparent.

[0067] The substrate 11 and the nano-groove mask structure 12 form an independent fluorescent nanoscale member, such as Figure 5 shown.

[0068] The nano-groove mask structure 12 includes nano-grooves 121, the shape of the nano-grooves 121 is wide lines and narrow spaces, and the size of the nano-grooves 121 is 200 nm.

[0069] The flow cell also in...

Embodiment 3

[0081] This embodiment provides a specific usage method of the fluorescent nanometer standard plate.

[0082] A fluorescent nanostandard plate with a flow cell, such as figure 1 As shown, it was fixed on the working platform of an inverted fluorescence microscope, and the focus was adjusted. Dissolve commercial fluorescein FITC in ethanol or a mixture of ethanol and water and draw it into a syringe. Connect the syringe containing fluorescein to the liquid inlet line 42, and use a manual or syringe pump to inject and fill the flow chamber 3;

[0083] The way to replace the fluorescein is to flush the flow chamber with a large amount of ethanol or a mixture of ethanol and water, and then pass in another fluorescein solution. To replace the third fluorescein, the steps are the same as above.

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Abstract

The invention relates to the technical field of biological medicine and advanced manufacture and relates to a fluorescent nanometer standard plate, a preparation method thereof and the application ofthe fluorescent nanometer standard plate. The fluorescent nanometer standard plate comprises a nanometer scale structure and a flow cell; the flow cell comprises a flow cavity; the flow cavity is arranged on the nanometer scale structure; the flow cell and the nanometer scale structure are combined, so that the fluorescent radiation of the nanometer scale structure is realized; the rapid replacement of various fluorescent substances is realized through the flow cell; and the nanometer scale structure can be repeatedly used. When the fluorescent nanometer standard plate is used, operation is simple and rapid, and flexibility is high. The preparation method of the fluorescent nanometer standard plate comprises the steps of preparing a nanometer scale structure and preparing a flow cell, wherein the flow cell can be prepared through a step-by-step preparation mode or an integral preparation mode. The preparation method of the invention is simple and has high precision. A fluorescent nanometer standard plate prepared through the method is high in sealing performance and rapid to use. The fluorescent nanometer standard plate has a bright application prospect in the field of fluorescenceanalysis.

Description

technical field [0001] The invention relates to the technical field of ultra-high resolution fluorescence microscopic imaging, in particular to a fluorescent nanometer standard plate and its preparation and application. Background technique [0002] Fluorescence microscopy plays an important role in the research of life sciences. It can study the life process and mechanism in the cell at the subcellular level. However, due to the limitation of the Abbe diffraction limit in traditional optics, its imaging resolution cannot exceed 200nm. the following. With the rise of ultra-high resolution fluorescence microscopy techniques, such as photosensitive localization microscopy (PALM), fluorescence photosensitive localization microscopy (FPALM), stochastic optical reconstruction microscopy (STORM), stimulated emission depletion microscopy, etc. (STED), Structured Illumination Microscopy (SIM) and Single Molecule Positioning Microscopy, etc. These techniques enable fluorescence micr...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/27
CPCG01N21/6458G01N21/278
Inventor 唐玉国张志强史国华蒋克明周武平刘聪张涛黎海文
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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