Cabbage type rape anti-sulfonylurea herbicide mutant gene and application thereof

Abstract

The invention provides a cabbage type rape anti-sulfonylurea herbicide mutant gene and application thereof. The cabbage type rape anti-sulfonylurea herbicide mutant gene has unit point mutation on twogenes of ALS1 and ALS3 separately, wherein the 1676 base in the coding sequence of the ALS1 gene is mutated from G to T, the 559 amino acid is mutated from tryptophan to leucine, and the specific amino acid sequence is shown in SEQ ID NO. 1; in the coding sequence of the ALS3 gene, the 1667 base is mutated from G to T, the 556 amino acid is mutated from tryptophan to leucine, andthe specific amino acid sequence is shown in SEQ ID NO. 2, a herbicide-resistant resistance site controlled by two pairs of dominant genes is transformed into a cabbage type rape recessive genic malesterility homozygous two-type line and a temporary maintainer line thereof, and a cabbage type rape herbicide-resistant recessive genic male sterility homozygous two-type line and a temporary maintainer line thereof are selected to be used for producing an all sterility line with a herbicide-resistant characteristic.

Description

technical field [0001] The invention belongs to the field of Brassica napus breeding, in particular to a mutation gene of Brassica napus resistant to sulfonylurea herbicides and its application. Background technique [0002] Recessive nuclear male sterility has become an important way to utilize rapeseed heterosis in China because of its complete and stable sterility and wide recovery source. When planting, 50% of the fertile plants in the sterile line need to be manually removed, which limits its wide application, such as double recessive genic male sterile S45A and 117A. [0003] 20118A is a new recessive nuclear male sterile line bred by researcher Sun Chaocai of Shanghai Academy of Agricultural Sciences in 1997. The sterile line has empty anthers, no pollen, bagged self-fertilization, and stable sterility. Not affected by environmental conditions. [0004] General genetics studies have proved that 20118A is controlled by 3 pairs of recessive genes. In order to facilita...

AI Technical Summary

Problems solved by technology

[0006] However, because the sterility traits and the traits of temporary insurance lines are controlled by recessive genes, they are easily affected by foreign pollen, mechanical mixing, etc., making it very difficult to obtain high-purity total sterile lines

The homozygous two-type line and the temporary insurance line are mixed with foreign hybrids or polluted by foreign pollen, so these hybrids cannot be effectively identified and eliminated

In addition, there are two ways to identify the purity of the CMS line. The first is to use specific molecular marker identification technology, which requires the identification unit to have molecular laboratories and related technical personnel; the second is to use the fertility of the flowering period Investigation and identification, this method requires low-temperature vernalization at the seedling stage, and can only be carried out after flowering, which takes a long period

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