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Porcine circovirus type 2/mycoplasma hyorhinis combined vaccine

A technology of porcine circovirus and mycoplasma hyorhina, which is applied in the field of porcine vaccines and can solve the problems of vaccine products without mycoplasma hyorhina

Pending Publication Date: 2019-08-16
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no commercially available vaccines against M. hyorhinis

Method used

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  • Porcine circovirus type 2/mycoplasma hyorhinis combined vaccine
  • Porcine circovirus type 2/mycoplasma hyorhinis combined vaccine
  • Porcine circovirus type 2/mycoplasma hyorhinis combined vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 porcine circovirus type 2 (PCV2) antigen

[0028] Viruses, Cells, Antibodies and Reagents:

[0029] Porcine circovirus type 2 LG strain was isolated from sick pigs with clinical manifestations of PMWS. PK15 cells were identified as being free from porcine circovirus type 2 and type 1 contamination. The standard positive serum of porcine circovirus type 2 LG strain was obtained from Harbin, China Preserved by the Institute of Veterinary Medicine.

[0030] Porcine circovirus type 2 LG strain culture:

[0031] 1. Full suspension culture of PK15 cells

[0032] 1.1 Optimization of the initial seeding density of cells Adjust the seeding density of PK15 cells to 10×10 3 / ml, 10×10 4 / ml, 30×10 4 / ml, 50×10 4 / ml, 80×10 4 / ml and 10×10 5 / ml, respectively in the bioreactor for full suspension culture (medium from Shanghai Yuanpei Biotechnology Co., Ltd. serum-free full suspension medium). The results showed that even if the cell seeding ...

Embodiment 2

[0068] The preparation of embodiment 2 mycoplasma hyorhina antigen

[0069] Viruses, cells, antibodies and reagents: Mycoplasma hyorhina DL strain was isolated from sick pigs with clinical manifestations of PMWS, and the standard positive serum of Mycoplasma hyorhina DL strain was preserved by Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0070] Culture of Mycoplasma hyorhina: Use the 10th generation culture of Mycoplasma hyorhina DL strain as the virus seed, inoculate it into the in vitro culture medium of Mycoplasma hyorhina synchronously (conventional substances such as PPLO broth, hydrolyzed milk protein and glucose) according to the dose of 0.2%, and place it at 37°C Shake culture under culture conditions for 40 h to harvest, measure CCU, centrifuge at 8000 rpm for 15 min, discard the supernatant and save the precipitate for later use.

[0071] Inactivation of Mycoplasma hyorhina: Recover the centrifugally purified Mycoplasma hyorhina p...

Embodiment 3

[0072] Example 3 Preparation of porcine circovirus type 2 / mycoplasma hyorhina dual vaccine

[0073] Utilize the water-based adjuvant according to the manufacturer's instruction method (the ratio of the mineral oil adjuvant to the water-phase antigen is 1:10, and the oil phase is slowly added to the water-phase antigen (porcine circovirus prepared respectively in Examples 1 and 2) 2 and Mycoplasma hyorhina), stirring at low shear for 30 minutes) to prepare the dual vaccine of porcine circovirus type 2 and Mycoplasma hyorhina (LG strain+DL strain). The sample of porcine circovirus type 2 / mycoplasma hyorhina dual vaccine was prepared, and the physical properties of the vaccine were tested, showing that the stability, viscosity, appearance and sterility test of the vaccine all met the requirements.

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Abstract

The invention relates to a multivalent immunogenicity composition which comprises a porcine circovirus type 2 (PCV2) antigen and a mycoplasma hyorhinis antigen. The composition can resist the attack of PCV2 and mycoplasma hyorhinis, and the immunized group and the healthy control do not have evident difference in terms of clinical pathology and anatomy pathology while evident clinical pathology and anatomy pathology appears in the challenge control. In addition, the mycoplasma hyorhinis can promote the titer of the PCV2 antibody, and the comprehensive control of diseases related to porcine circovirus can be promoted.

Description

technical field [0001] The invention relates to a vaccine for pigs, and further relates to a combination vaccine of porcine circovirus type 2 (PCV2) and mycoplasma hyorhina. Background technique [0002] Porcine circovirus (PCV) is a member of the genus Circovirus (Circovirus) of the family Circoviridae, and is divided into porcine circovirus type 1 (PCV1) and pig Circovirus type 2 (Porcine circovirus type 2, PCV2). Among them, PCV1 is not pathogenic to pigs, while PCV2 is pathogenic and is the main pathogen that causes porcine circovirus-associated diseases (PCVAD), such as sow reproductive disorders (PCV2-associated reproductive disorders), pig respiratory Disease syndrome (Porcine respiratory disease complex, PRDC), enteritis (Enteritis), porcine dermatitis and nephrotic syndrome (Porcine dermatitis and nephropathy syndrome, PDNS), proliferation and necrotizing pneumonia (Proliferative and necrotizing pneumonia, PNP), etc. PCV2 is the most important pathogen in these di...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/12A61K39/02A61K39/39A61P31/20A61P31/04
CPCA61K39/12A61K39/0241A61K39/39A61P31/20A61P31/04C12N2750/10034A61K2039/70A61K2039/55505A61K2039/55566A61K2039/55511Y02A50/30
Inventor 危艳武刘长明黄立平吴洪丽冯力
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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