Preparation method of 3D bioprinting ink with adjustable gel-sol phase transition temperature

A phase transition temperature, 3D printing technology, used in tissue regeneration, prosthesis, medical science, etc., can solve the problems of mismatched cell growth temperature and poor mechanical properties of hydrogel materials, and achieve enhanced mechanical properties and balance accuracy. , The effect of the simple process

Active Publication Date: 2021-10-29
HANGZHOU DIANZI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adding inorganic nanoparticles to this basic hydrogel system can improve the mechanical properties of the cartilage scaffold, thereby solving the technical problems of the mismatch between the printing temperature and the cell growth temperature and the poor mechanical properties of the hydrogel material

Method used

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  • Preparation method of 3D bioprinting ink with adjustable gel-sol phase transition temperature
  • Preparation method of 3D bioprinting ink with adjustable gel-sol phase transition temperature
  • Preparation method of 3D bioprinting ink with adjustable gel-sol phase transition temperature

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Swell 4g of gelatin powder in a beaker filled with 20ml of deionized water for 30min, put the second beaker into a water bath magnetic stirrer with a water temperature of 78°C and heat and stir at a speed of 20r / min for 30min to obtain a concentration of 20.0% (W / V) gelatin aqueous solution, add 1g sodium alginate powder, 25ml deionized water in the beaker that fills gelatin aqueous solution, the heating temperature of magnetic stirrer keeps constant, and the rotating speed is adjusted to 10r / min, heating and stirring 80min, finally Obtain the sodium alginate of concentration about 2.2% (W / V) and the gelatin composite solution of concentration about 8.8% (W / V); Gradually lower to 20°C, take out and refrigerate for later use. 3D cartilage hydrogel scaffolds were printed at room temperature using a cell-controlled assembly machine. Place the printed scaffold in 4% CaCl 2 cross-linked in solution, the scaffold was removed from the CaCl 2 The solution was take...

Embodiment 2

[0039] Embodiment 2: 4g gelatin powder is filled in the beaker of 20ml deionized water and swells 30min, and the beaker is put into the water bath magnetic stirrer that water temperature is 78 ℃ and is heated and stirred at the speed of 20r / min for 30min to make concentration be 20.0% ( W / V) gelatin aqueous solution, add 1g sodium alginate powder, 25ml deionized water in the beaker that is filled with gelatin aqueous solution, the heating temperature of magnetic stirrer keeps constant, and the rotating speed is adjusted to 14r / min, heats and stirs 90min, finally obtains A compound solution of sodium alginate with a concentration of about 2.2% (W / V) and gelatin with a concentration of about 8.8% (W / V); adjust the heating temperature of the magnetic stirrer to 28°C, and set the speed to 0r / min, and compound The temperature of the solution gradually decreased to 28°C along with the water temperature; again, the heating temperature was set to 78°C, and the rotation speed was set to...

Embodiment 3

[0040]Example 3: Swell 4g of gelatin powder in a beaker filled with 20ml of deionized water for 30min, put the second beaker into a water bath magnetic stirrer with a water temperature of 78°C and heat and stir at a speed of 20r / min for 30min to obtain a concentration of 20.0% (W / V) gelatin aqueous solution, add 1g sodium alginate powder, 25ml deionized water in the beaker that is filled with gelatin aqueous solution, the heating temperature of magnetic stirrer keeps constant, and the rotating speed is adjusted to 14r / min, heating and stirring for 90min, finally Obtain a sodium alginate with a concentration of about 2.2% (W / V) and a gelatin composite solution with a concentration of about 8.8% (W / V); the heating temperature of the magnetic stirrer is adjusted to 28°C, and the rotating speed is set to 0r / min, The temperature of the composite solution gradually decreased to 28°C along with the water temperature; the heating temperature was set to 78°C again, the rotation speed wa...

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Abstract

The invention discloses a preparation method of biological 3D printing ink that can regulate the gel-sol phase transition temperature. The invention controls the sol-gel phase transition temperature of the gelatin / sodium alginate 3D printing hydrogel system. In the temperature range most suitable for cell growth. Then, a high-resolution cartilage scaffold was printed using cell 3D printing technology to improve the printability and biocompatibility of the hydrogel material. Adding inorganic nanoparticles to this basic hydrogel system can improve the mechanical properties of the cartilage scaffold, thereby solving the technical problems of the mismatch between the printing temperature and the cell growth temperature and the poor mechanical properties of the hydrogel material.

Description

technical field [0001] The invention relates to the technical field of material preparation of artificial cartilage scaffolds prepared by biological 3D printing, in particular to a method for preparing cartilage scaffolds with sodium alginate / gelatin-based printing ink that can regulate the sol-gel transition temperature. Background technique [0002] There are more and more patients with cartilage defects caused by obesity, exercise, age or disease, and it has become a major killer of human health. At present, cartilage repair is still a major problem in clinical medicine. In recent years, the development of cell 3D printing technology has promoted the application of additive manufacturing technology in the field of tissue engineering. Cell 3D printing technology not only regulates the mechanical properties of the scaffold by controlling the microstructure, shape and composition of the tissue scaffold with high precision, but also has a certain impact on the number and dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/26A61L27/52C08J3/075C08L89/00C08L5/04
CPCA61L27/26A61L27/52A61L2430/06C08J3/075C08J2389/00C08J2405/04C08L5/08C08L89/00
Inventor 史廷春李美琪姚丹语
Owner HANGZHOU DIANZI UNIV
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