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Method for improving heat resistance of lactic acid bacteria and application

A heat-resistant, lactic acid bacteria technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem that the effect of heat resistance is not strong, the advantages are not clearly reflected, and the number of viable bacteria is reduced. and other problems to achieve the effect of improving heat resistance, activity and stability, and ensuring effective performance

Active Publication Date: 2019-08-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of its production and storage, it often faces stress environments such as heat and high osmotic pressure, resulting in a decrease in the number of viable bacteria.
The effect of a single stress on improving heat resistance is not strong, and the advantages cannot be clearly reflected in practical applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for improving heat resistance of lactic acid bacteria, comprising the steps of:

[0027] The slant medium, seed medium and fermentation medium involved in this embodiment are all MRS;

[0028] 1) Preparation of seed liquid: Streak and activate lactic acid bacteria frozen at -80°C on a solid medium, pick a single colony and inoculate it in MRS liquid medium, culture at 37°C for 16 hours, and then add 2% (v / v) Inoculate the inoculation amount of the seed liquid into the seed medium, and cultivate it for 12 hours as the seed liquid; inoculate the seed liquid in the fresh MRS medium with the inoculum amount of 2% (v / v), and culture it statically for 15 hours at 37°C. At this time, the bacterial concentration is 1.23 ×10 9 CFU / ml. The heat resistance of the starting strain was tested, and the survival rate was 0.62%. The lactic acid bacteria are: Lactobacillus casei (Lactobacillus casei solid-state fermentation and drying technology research, Xie Yitian, 2013). ...

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Abstract

The invention belongs to the technical field of lactic acid bacteria processing and particularly relates to a method for improving heat resistance of lactic acid bacteria and application. After acid stress and temperature stress of lactic acid bacteria under appropriate conditions, a bacterial protecting agent is added to substantially improve heat resistance of lactic acid bacteria. Compared withan original strain, heat resistance of the treated lactic acid bacteria is improved by more than 80 times, and the method is quite suitable for extensive popularization.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria processing, and in particular relates to a method and application for improving the heat resistance of lactic acid bacteria. Background technique [0002] Lactic acid bacteria are a class of Gram-positive bacteria capable of producing lactic acid. They are the most important industrial microorganisms and are widely used in food processing. Most lactic acid bacteria have probiotic effects, which can promote the body's metabolism, help digestion and absorption, inhibit exogenous pathogens, regulate the proportion of intestinal flora, improve the intestinal micro-ecological environment, and prevent and treat diseases. [0003] Studies have shown that adding a certain concentration of Ca during the cultivation of lactic acid bacteria 2+ , can improve the stability of the cell membrane, making it difficult to be damaged in a hot environment, and protecting cell activity. Physical mutagen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/225C12R1/23C12R1/245
CPCC12N1/04C12N1/20
Inventor 安璟梁运祥吴鹏宇卢立轩陈亮臧琬婷艾洪超
Owner HUAZHONG AGRI UNIV