Magnetic nanofiber-based zwitterionic hydrophilic materials for selective capture and recognition of glycopeptides
A technology of hydrophilic materials and zwitterions, applied in the field of biochemical analysis, can solve the problems of increasing the cost of analysis methods, complex synthesis procedures, long incubation time, etc., and achieve strong binding ability, high enrichment selectivity, and good recovery rate Effect
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[0059] In order to make the technical solution of the present invention clearer, the present invention is further described below, and any solution obtained by equivalent replacement and conventional reasoning of the technical features of the technical solution of the present invention falls within the protection scope of the present invention.
[0060] A magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides using hydroxyapatite nanofibers as a carrier to immobilize Fe 3 o 4 Nanoparticles and Au nanoparticles, through the thiol group in the zwitterionic tripeptide L-glutathione interacting with Au and Fe 3 o 4 The affinity between the surface modification, the formation of mHN / Au-GSH nanofibers.
[0061] Further, the preparation method of mHN / Au-GSH nanofibers is as follows:
[0062] S1: Disperse 2.0g of hydroxyapatite nanofibers and 0.75g of iron acetylacetonate in 400mL of triethylene glycol to obtain a mixture; ...
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