A kind of test kit for Staphylococcus wauserii detection

Abstract

The present invention provides a kit for detecting staphylococcus warneri. The kit comprises a guide RNA specifically targeting a staphylococcus warneri rpoB gene, an amplification primer pair, hydration TwistAmp basic kit reaction drying spheres, LbCas12a protein, a single-stranded DNA probe (ssDNA), an ribonuclease inhibitor and a buffer solution. The kit is used for detecting the staphylococcuswarneri and high in detection specificity, and can separate the staphylococcus warneri from other staphylococci, including staphylococcus aureus, staphylococcus hominis, staphylococcus epidermidis, staphylococcus capitis and staphylococcus haemolyticus. At the same time, the use of the RNA sequence for the detection takes about 1 hour, does not require a PCR instrument, has low requirements on equipment, and is significantly lower than a conventional bacterial culture method or a PCR sequencing method and large in clinical practical value.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for the detection of Staphylococcus worbachii, comprising a guide RNA specifically targeting the rpoB gene of Staphylococcus worbachii, a pair of RPA amplification primers, and a hydrated TwistAmp basickit Reaction dry balls, LbCas12a protein, single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor, and buffer. This kit can be used for rapid detection of Staphylococcus wausii. Background technique [0002] Staphylococcus worthii is a common bacterial group among Gram-positive bacteria that infects human beings, which brings a huge burden to patients and society. The traditional detection method is to carry out bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus wordii from other staphylococci due to the lack of specific primers, so joint generation sequencing is required It can ...

AI Technical Summary

Problems solved by technology

The traditional detection method is to carry out bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus wausii from other staphylococci due to the lack of specific primers, so joint generation sequencing is required Can only be identified, the cost is high, and it takes several days to get the result

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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