LC-MS method for the detection of various marine biotoxins in aquatic products

A technology for marine organisms and LC/MS, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of affecting the detection effect, the purification effect not meeting the requirements, and the loss.
CN110146632BActive Publication Date: 2021-09-21NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
3 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
Publication Date
2021-09-21

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a liquid mass spectrometry detection method for a variety of marine biological toxins in aquatic products. The samples of the aquatic products to be tested are extracted with 0.1vol% formic acid aqueous solution and acetonitrile in multiple stages, and the upper organic phase and the lower aqueous phase are obtained by layering. ; The lower aqueous phase was added to the MCX-HLB series column, rinsed with low-proportion methanol water, and then eluted once with the organic phase purified by dSPE, and then eluted with basic methanol for the second time. Liquid chromatography-tandem mass spectrometry is used for detection, and the external standard method is used for quantification to obtain the content of each component to be tested in the aquatic product sample to be tested. The invention can detect 8 kinds of marine biotoxins including hydrophilic and lipophilic at one time, and for the first time realizes one-step liquid mass spectrometry high-throughput quantitative detection of hydrophilic and lipophilic marine biotoxins.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a liquid-mass spectrometry detection method for various marine biotoxins in aquatic products Background technique

[0002] With the progress of society and the development of industry, environmental problems are becoming more and more serious. Some industrial waste water and domestic sewage enter rivers, lakes and seas, resulting in eutrophication of water bodies and the proliferation of algae in water bodies. In particular, many marine red tide algae can produce toxins and accumulate in shellfish and other marine animals. Seafood contaminated by toxins can harm the digestive system, nervous system or cardiovascular system in the human body after being eaten by consumers, leading to human poisoning. even death. Common types of marine biotoxins in commercially available seafood include Paralytic Shellfish Poisoning (PSP), Diarrhetic Shellfish Poisoning (DSP), Amnesic Shellfish Poisoning (ASP), nervous Neurotoxic Shellfish Pois...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More