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Compound nanometer micelle for packaging dye J aggregate and preparing method and application of micelle

A technology of nanomicelles and aggregates, applied in the field of medicine, can solve problems such as poor stability in vivo, long preparation time, and unsatisfactory yield, and achieve good thermal stability and outstanding structural stability.

Inactive Publication Date: 2019-08-23
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method for directly preparing ICG J aggregates developed by it still has problems such as long preparation time, poor stability in vivo, and difficulty in subsequent multifunctional modification, and the yield of this method is not ideal.

Method used

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  • Compound nanometer micelle for packaging dye J aggregate and preparing method and application of micelle
  • Compound nanometer micelle for packaging dye J aggregate and preparing method and application of micelle
  • Compound nanometer micelle for packaging dye J aggregate and preparing method and application of micelle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This embodiment provides a method for inducing J aggregation of the dye indocyanine green (ICG), that is, preparing a composite nanomicelle containing ICG J aggregates. The specific preparation method is:

[0070] The dye ICG (0.05mg / mL) and DSPE-PEG2000-HN 2 (0.1mg / mL) was co-dissolved in ultrapure water with a pH value of 7.5 and stirred at 25°C for 48h to obtain the composite nanomicelles loaded with dye J aggregates, using DSPE-PEG@ICG-J- 1 indicates that the obtained DSPE-PEG@ICG-J-1 was concentrated by ultrafiltration and then stored in the dark at 4°C.

[0071] The detection of the absorption spectrum of the solution system at different time points in the above stirring process is as follows: figure 1 Shown: It can be seen that most of the ICG has been converted into J aggregates at 12 hours, and the proportion of J aggregates is also higher as the stirring time is prolonged.

Embodiment 2

[0073] This example provides a method for inducing J aggregation of the dye ICG, that is, preparing a composite nanomicelle containing ICG J aggregates. The specific preparation method is as follows:

[0074] The dye ICG (0.5mg / mL) and DSPE-PEG2000-HN 2 (0.5mg / mL) was co-dissolved in ultrapure water with a pH value of 8.5, first stirred at 80°C for 15min, and then stirred at 25°C for 8h to obtain the composite nanomicelles loaded with dye J aggregates, Expressed as DSPE-PEG@ICG-J-2, the resulting DSPE-PEG@ICG-J-2 was concentrated by ultrafiltration and stored at 4°C in the dark.

[0075] The detection of the absorption spectrum of the solution system at different time points in the above stirring process is as follows: figure 2 Shown: It can be seen that ICG has been basically transformed into J aggregates at 8h.

Embodiment 3

[0077] This example provides a method for inducing J aggregation of the dye ICG, that is, preparing a composite nanomicelle containing ICG J aggregates. The specific preparation method is as follows:

[0078] The dye ICG (1mg / mL) and DSPE-PEG2000-HN 2 (0.5mg / mL) was co-dissolved in phosphate buffer with a pH value of 4.0, stirred at 25°C for 8h to obtain the composite nanomicelles loaded with dye J aggregates, and DSPE-PEG@ICG-J -3 indicates that the obtained DSPE-PEG@ICG-J-3 was concentrated by ultrafiltration and then stored in the dark at 4°C.

[0079] The solution system when 8h in the above-mentioned stirring process is carried out the detection of absorption spectrum, the result is as follows image 3 Shown: It can be seen that ICG has been basically transformed into J aggregates at 8h.

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Abstract

The invention relates to compound nanometer micelle for packaging a dye J aggregate and a preparing method and application of the micelle. The compound nanometer micelle comprises an amphipathic polymer carrier and the dye J aggregate packaged in the amphipathic polymer carrier. The tail end of the hydrophilic segment of an amphipathic polymer is provided with an electrified group. A dye is a water-soluble amphipathic dye. By means of the compound nanometer micelle, he dye can be located in the micelle in the form of the J aggregate; the dye J aggregate has outstanding structural stability; the micelle has an active reaction group, further modification is convenient, and the medicine packaging can be realized. The preparing method of the micelle is not limited by the concentration of the dye, and the dye with the low mass concentration can form the J aggregate at a high yield within a short period of time without long-term heating or dialysis or other operations.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a composite nanomicelle carrying dye J aggregates and a preparation method and application thereof. Background technique [0002] In view of outstanding biodegradability, biocompatibility, and biosafety, organic biomedical materials have developed very rapidly in recent years. The wide application of organic biomedical materials based on organic dye molecules in some new optical-related diagnostic methods, such as photothermal therapy (PTT) and photoacoustic imaging (PAI), as well as the huge application potential of these diagnostic methods, have great impact on the related materials. Preparation and application research has received more and more attention. However, these dye molecules used in PTT, PAI and other fields often show poor structural stability in the free state, and are easy to decompose and deteriorate under the action of light, heat and enzymes, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/107A61K47/60A61K49/00A61K49/22A61P35/00A61K31/704
CPCA61K9/1075A61K31/704A61K41/0052A61K49/0034A61K49/227A61K47/60A61P35/00A61K2300/00
Inventor 田雷蕾邵晨肖凡于建涛
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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