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Bacillus licheniformis, screening method, application and feed containing bacillus licheniformis

A technology of Bacillus licheniformis and screening method, which is applied in the field of microbial screening and application, can solve the problems of poor palatability, difficulty in long-term storage, and high ratio of mulberry leaf feed, and achieve the effects of improving the quality of feed mulberry silage, reducing the degree of decomposition, and improving digestion

Inactive Publication Date: 2019-08-23
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mulberry has been cultivated in my country for more than 5,000 years, and has the largest planting area in China. Mulberry leaves are rich in nutrients such as protein, vitamins, polysaccharides, and mineral elements. The protein content of mulberry leaves is relatively high, about 15-30%. Fresh mulberry leaves have high water content and are difficult to preserve for a long time. At present, silage is the most suitable storage method for them. However, the mulberry leaf feed stored in this way has poor palatability, low protein utilization rate, high ratio of ammonia nitrogen to total nitrogen, Crude fiber cannot be effectively degraded into sugars, making mulberry leaves not widely fed to animals as a routine feed

Method used

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  • Bacillus licheniformis, screening method, application and feed containing bacillus licheniformis
  • Bacillus licheniformis, screening method, application and feed containing bacillus licheniformis
  • Bacillus licheniformis, screening method, application and feed containing bacillus licheniformis

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Comparison scheme
Effect test

Embodiment 1

[0041] Such as figure 1 Shown, a kind of screening method of bacillus licheniformis comprises the following steps:

[0042] S1, collect soil samples: adopt three-point method to collect 90g of mulberry leaf planting soil;

[0043] Such as figure 2 Shown, the concrete steps of described three-point method collecting mulberry leaf planting land soil are:

[0044]S101. Select the densely grown soil of mulberry leaves in the north, southwest and southeast of the mulberry leaf planting base respectively, and the sampling points are as follows: figure 2 Shown by the black dots in ●;

[0045] S102. Collect soil samples in the north, southwest and southeast of the sampling point ●, such as figure 2 As shown in the hollow circle О, and mix well.

[0046] S2. Cultivation and separation of bacterial strains: put the collected soil samples in a sterile Erlenmeyer flask filled with 800ml of PBS, shake well for 18 minutes to dilute the soil samples into a sample suspension, and then...

Embodiment 2

[0071] Such as figure 1 Shown, a kind of screening method of bacillus licheniformis comprises the following steps:

[0072] S1, collect soil sample: adopt three-point method to collect 110g of mulberry leaf planting soil;

[0073] Such as figure 2 Shown, the concrete steps of described three-point method collecting mulberry leaf planting land soil are:

[0074] S101. Select the densely grown soil of mulberry leaves in the north, southwest and southeast of the mulberry leaf planting base respectively, and the sampling points are as follows: figure 2 Shown by the black dots in ●;

[0075] S102. Collect soil samples in the north, southwest and southeast of the sampling point ●, such as figure 2 As shown in the hollow circle О, and mix well.

[0076] S2. Cultivation and separation of bacterial strains: put the collected soil samples in a sterile Erlenmeyer flask filled with 1000ml of PBS, shake well for 22 minutes to dilute the soil samples into a sample suspension, and th...

Embodiment 3

[0101] Such as figure 1 Shown, a kind of screening method of bacillus licheniformis comprises the following steps:

[0102] S1, collect soil sample: adopt three-point method to collect 100g of soil of mulberry leaf planting place;

[0103] Such as figure 2 Shown, the concrete steps of described three-point method collecting mulberry leaf planting land soil are:

[0104] S101. Select the densely grown soil of mulberry leaves in the north, southwest and southeast of the mulberry leaf planting base respectively, and the sampling points are as follows: figure 2 Shown by the black dots in ●;

[0105] S102. Collect soil samples in the north, southwest and southeast of the sampling point ●, such as figure 2 As shown in the hollow circle О, and mix well.

[0106] S2. Cultivation and separation of bacterial strains: put the collected soil samples in a sterile Erlenmeyer flask filled with 900ml of PBS, shake well for 20 minutes to dilute the soil samples into a sample suspension...

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Abstract

The invention discloses bacillus licheniformis, a screening method, application and a feed containing the bacillus licheniformis. The bacillus licheniformis is reserved at a China center for type culture collection, and the reservation number is CCTCC M 2019165. The screening method comprises the steps of collecting soil samples, culturing and dividing bacterial strains, separating and purifying the bacillus licheniformis, conducting molecular identification, conducting biochemical identification, and identifying and screening out the bacillus licheniformis according to a molecular identification result and a biochemical identification result. The screened bacillus licheniformis is used for preparing mulberry silage, the protein content of the feed is increased so that animals can have sufficient nitrogen source supply, the content of crude fiber needs to be reduced so that the animals can have more easily decomposed carbohydrates, the ratio of ammoniacal nitrogen to total nitrogen needs to be reduced so that the protein of the silage and the decomposition degree of amino acid can be reduced, and thus the silage with better quality can be formed.

Description

technical field [0001] The invention relates to the technical field of microbial screening and application, in particular to a bacillus licheniformis, a screening method, an application and a feed containing the bacillus licheniformis. Background technique [0002] Mulberry has been cultivated in my country for more than 5,000 years, and has the largest planting area in China. Mulberry leaves are rich in nutrients such as protein, vitamins, polysaccharides, and mineral elements. The protein content of mulberry leaves is relatively high, about 15-30%. Fresh mulberry leaves have high water content and are difficult to preserve for a long time. At present, silage is the most suitable storage method for them. However, the mulberry leaf feed stored in this way has poor palatability, low protein utilization rate, high ratio of ammonia nitrogen to total nitrogen, Crude fiber cannot be effectively degraded into sugars, making mulberry leaves not widely fed to animals as a routine feed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/10
CPCA23K30/18C12N1/20C12N1/205C12R2001/10
Inventor 朱佳文许祯莹吴永胜邱时秀雷春龙陈亚迎徐麒麟刘瀚扬李娟齐桂兰杨雪曹雨辰
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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