Detection method of types of CYP2D6 gene

A detection method and genotyping technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of long detection cycle, high cost, and low efficiency of CYP2D6 genotyping detection
CN110157779AInactive Publication Date: 2019-08-23BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
Publication Date
2019-08-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a detection method of types of a CYP2D6 gene. The detection method comprises the steps of preparing a first polymerase chain reaction (PCR) amplification system comprising specific amplification primers shown in SEQ ID NO.1-14 and a second PCR amplification system comprising specific amplification primers shown in SEQ ID NO.15-28, carrying out amplification reactions in sequence, and then conducting digestive treatment; obtaining a first digestion reaction system and a second digestion reaction system; preparing a first single base extension reaction system comprising single base extension primers shown in SEQ ID NO.29-49 and a second single base extension reaction system comprising single base extension primers shown in SEQ ID NO.50-69; carrying out extension reactions on the first digestion reaction system joining in the first single base extension reaction system and the second digestion reaction system joining in the second single base extension reaction system, then conducting purification treatment, sequentially utilizing a detection instrument comprising a mass spectrometer for detection, and determining genotypes of SNP loci of the CYP2D6 gene of a to-be-detected sample. By means of the detection method, the detection efficiency of the types of the CYP2D6 gene can be improved.
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Description

technical field

[0001] The invention relates to the technical field of gene detection, in particular to a detection method for CYP2D6 genotyping. Background technique

[0002] Cytochrome P450 (CYP450) is a gene family composed of heme-thiolate proteins, which can participate in the synthesis of sterol hormones and the metabolism of endogenous and exogenous substances in organisms. CYP2D6 gene is one of the members of CYP450 family, which is of great significance in genetic pharmacology.

[0003] Currently, NGS is usually used to detect CYP2D6*2, *3, *4, *5, *6, *7, *8, *9, *10, *11, *12, *13, *14, * 15, *16, *17, *19, *20, *21, *29, *33, *35, *36, *38, *40, *41, *42 genotypes. However, the detection cycle of the next-generation sequencing method is long and the cost is high, which leads to low detection efficiency of CYP2D6 genotyping. Contents of the invention

[0004] The embodiment of the present invention provides a detection method of CYP2D6 genotyping, which can i...

Claims

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