A method for tissue culture and rapid propagation of Zhongjiang salvia miltiorrhiza
A technology of tissue culture and rapid propagation of Salvia miltiorrhiza, applied in the field of tissue culture and seedling cultivation of medicinal plants, can solve the problems of many diseases and insect pests, a large amount of seeds required, and degradation of reproductive quality, and achieve good growth status, high yield, hardening and transplanting of seedlings. The effect of high survival rate
Active Publication Date: 2022-04-08
SICHUAN AGRI UNIV
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[0003] The purpose of the present invention is to provide a method for tissue culture and rapid propagation of Salvia miltiorrhiza in order to solve the problems in the prior art that the reproduction quality is degraded, the amount of seeds required is large, and there are many pests and diseases.
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[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0024] see figure 1 , the present invention provides a technical solution: a method for tissue culture and rapid propagation of Zhongjiang Salvia Miltiorrhiza, the steps are as follows:
[0025] S1, material processing: select Chuandan roots with healthy and uniform growth, and obtain aseptic materials after sterilization;
[0026] S2, inoculate the material on the primary culture medium and cultivate it for about 5 days, and transfer it to the bud induction ...
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The invention relates to a method for tissue culture and rapid propagation of Zhongjiang Salvia miltiorrhiza, the steps are as follows: S1, material processing: select the root of "Chuan Danshen No. 1" with healthy and uniform growth, and obtain aseptic materials after sterilization; S2, inoculate the materials Cultivate on the first-generation culture medium for about 5 days, and transfer to the bud induction medium after confirming that there is no fungal and bacterial contamination; S3, transfer the root segment to the bud induction medium and cultivate it for about 25 days until cluster buds are formed; S4, the step The clustered shoots obtained in S3 were cut into individual rootless tissue cultured seedlings, transferred to the strong seedling medium, and cultivated for about 14 days; S5, the strengthened tissue cultured plantlets were inserted into the rooting medium to induce rooting S6, rooted The latter tissue-cultured seedlings were transplanted for hardening. The method of tissue culture and rapid propagation of Zhongjiang Salvia miltiorrhiza uses roots as explants, which completely avoids the common browning problem in the tissue culture of Salvia miltiorrhiza, and does not add sucrose to the medium at each stage, which can promote the growth of tissue culture seedlings in the culture stage. Photosynthesis is self-supporting, effectively increasing the growth ability of seedlings.
Description
technical field [0001] The invention relates to the technical field of tissue culture and seedling cultivation of medicinal plants, in particular to a method for tissue culture and rapid propagation of Zhongjiang salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza Bunge is a perennial herb of the genus Salvia L. in the family Lamiaceae. It is used as medicine with dried roots and rhizomes. It is mainly used to treat various cardiovascular and cerebrovascular diseases such as coronary heart disease, angina pectoris and stroke, as well as chronic hepatitis, early liver cirrhosis, vasculitis, diabetes complications, etc. It is the first choice for the treatment of cardiovascular and cerebrovascular diseases recognized worldwide. As the authentic and main production area of Salvia miltiorrhiza, the salvia miltiorrhiza produced in Sichuan Province is dubbed "Chuan Danshen" in the market circulation. Salvia miltiorrhiza (also known as "Zhongjiang salvia milti...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张利胡佳姜媛媛倪苏王龙况思雨陈欢敖青霞王海平吴金勇
Owner SICHUAN AGRI UNIV