Method for producing gamma-polyglutamic acid by solid state fermentation of soy sauce residue

A solid-state fermentation, polyglutamic acid technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve problems such as environmental pollution, untimely treatment of soy sauce residue, etc., to reduce production costs and promote comprehensive development. , the effect of increasing production

Inactive Publication Date: 2019-09-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to overcome the deficiencies in the prior art above, the present invention provides a method for producing γ-polyglutamic acid by solid-state fermentation of soy sauce residue, using agricultural waste auxiliary materials such as soy sauce residue, bran, and rice bran as solid-state fermentation substrates to produce γ-polyglutamic acid. Polyglutamic acid can not only produce γ-polyglutamic acid, but also solve the problem of environmental pollution caused by untimely or improper disposal of soy sauce residue, and turn soy sauce residue into treasure

Method used

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Examples

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Comparison scheme
Effect test

example 1

[0034] Activation culture of Bacillus licheniformis. This technology is an existing technology, and the specific process is as follows: the first-level slant adopts LB slant, cultures the preserved bacteria on the LB slant at 37°C for 24h, inoculates it into the seed medium, and cultivates it in a shaker flask at 37°C for 12-18 hours to The logarithmic phase of growth is used as a solid fermentation strain for future use. The preserved strains were provided by Tianjin Beiyang Baichuan Biotechnology Co., Ltd. The composition of LB medium is peptone 1%, yeast extract 0.5%, sodium chloride 1%, natural pH.

[0035] 20g of soy sauce residue and 20g of bran were used as solid fermentation base material; 2.4g of glucose, 2.4g of sodium glutamate, K 2 SO 4 0.4g, (NH 4 ) 2 SO 40.2g, fully dissolved in 40ml of water, adjusted to pH 7.2 with HCl or NaOH aqueous solution, and used as a nutritional supplement; crushed soy sauce residue, passed through a 20-mesh sieve and mixed with b...

example 2

[0039] Activation and cultivation of Bacillus licheniformis. The primary slant adopts LB slant, cultivated at 37°C for 24 hours, inoculated into the seed medium, and cultivated in shake flasks at 37°C for 12-18 hours until the growth logarithmic phase is used as a solid fermentation strain for later use. The preserved strains were provided by Tianjin Beiyang Baichuan Biotechnology Co., Ltd. The composition of LB medium is peptone 1%, yeast extract 0.5%, sodium chloride 1%, natural pH.

[0040] 20g of soy sauce residue and 20g of bran were used as solid fermentation base material; 3.2g of glucose, 2.4g of sodium glutamate, K 2 SO 4 0.8g, (NH 4 ) 2 SO 4 0.4g, fully dissolved in 40ml of water, adjusted to pH 7.0 with HCl or NaOH aqueous solution, and used as a nutritional supplement for later use; crushed soy sauce residue, sieved and mixed with bran as a solid fermentation base material; the above solid fermentation base The nutritional supplements are mixed with the nutri...

example 3

[0044] Activation and cultivation of Bacillus licheniformis. The primary slant adopts LB slant, cultivated at 37°C for 24 hours, inoculated into the seed medium, and cultivated in shake flasks at 37°C for 12-18 hours until the growth logarithmic phase is used as a solid fermentation strain for later use. The preserved strains were provided by Tianjin Beiyang Baichuan Biotechnology Co., Ltd. The composition of LB medium is peptone 1%, yeast extract 0.5%, sodium chloride 1%, natural pH.

[0045] 20g of soy sauce residue and 20g of bran were used as solid fermentation base material; 3.2g of glucose, 2g of sodium glutamate, K 2 SO 4 0.4g, (NH4) 2 SO 4 0.4g, fully dissolved in 40ml of water, adjusted to pH 7.0 with HCl or NaOH aqueous solution, and used as a nutritional supplement for later use; crushed soy sauce residue, passed through a 20-mesh sieve and mixed with bran as a solid fermentation base material; The fermentation base material is mixed with nutritional supplement...

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Abstract

The invention belongs to the technical field of bioengineering fermentation, in particular to a method for producing gamma-polyglutamic acid by solid-state fermentation of soy sauce residue. The method comprises the following steps: preparing a solid fermentation medium for soy sauce residue and performing activating culture of Bacillus licheniformis strain; inoculation and performing solid-statefermentation processes: inoculating Bacillus licheniformis in the solid fermentation medium prepared in step 1 for solid-state fermentation, and producing the gamma-polyglutamic acid; extraction of gamma-polyglutamic acid: performing separating and purification on the medium containing gamma-polyglutamic acid in step 2 to obtain the purified gamma-polyglutamic acid. The method has the beneficial effects that: soy sauce residue and agricultural waste auxiliary materials such as bran and gluten are used as a solid fermentation substrate to produce gamma-polyglutamic acid, the gamma-polyglutamicacid is prepared, the environmental pollution problem caused by untimely or improper treatment of soy sauce residue can be solved, and the soy sauce residue turns into treasure.

Description

technical field [0001] The invention belongs to the technical field of bioengineering fermentation, in particular to a method for producing gamma-polyglutamic acid by solid-state fermentation of soy sauce residue. Background technique [0002] γ-polyglutamic acid, with a molecular weight of 10-1000kDa, is used in many fields. It can be used in antifreeze, thickener, astringent, etc. in food, and it can be used in moisturizing masks in the cosmetic industry. In the pharmaceutical industry, it can be used as a drug carrier, adhesive, etc. In agriculture, it has good water retention for soil plants, and can also be used as a fertilizer synergist. It can be used as a polymer bioflocculant and heavy metal adsorption in industry. It can be seen that γ-polyglutamic acid has good application prospects in many fields. The production methods of γ-polyglutamic acid currently studied are mostly liquid fermentation. The cost of liquid fermentation is high. The fermentation process is li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12R1/10
CPCC12P13/02
Inventor 乔长晟姜美求牛桢桢赵廷彬
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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