A method for accurate identification of Pythium soybean root rot resistance resources
A root rot and resource technology, applied in the field of plant germplasm resource utilization research, can solve problems such as large gaps, and achieve the effect of simple method, enhanced reliability and accuracy
Active Publication Date: 2021-11-05
FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI
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Problems solved by technology
[0004] The United States successfully applied 9 Phytophthora root rot resistance genes to commercial breeding, which significantly improved the variety resistance. There is a big gap between my country and foreign countries in the cultivation of new disease-resistant soybean varieties, and new breakthroughs are urgently needed
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Embodiment 1
[0033] A method for identifying soybean germplasm resources resistant to Pythium root rot by using rice grain culture medium inoculation method. The rice grain medium inoculation method utilizes high-temperature sterilized rice grains to inoculate Pythium spp., then inoculates soybean germplasm resource seeds with the cultivated inoculum, uses vermiculite as a matrix to cultivate in a greenhouse, and investigates and grades the diseases of the plants. And calculate fresh weight ratio, by carrying out variance analysis to plant fresh weight ratio (Ratio) and disease index (Disease Index), carry out cluster analysis to plant fresh weight ratio (Ratio) and disease index (Disease Index), screen resistance and Sensitive resource, identification of new accessions with resistance / tolerance to Pythium root rot. (Such as figure 1 shown).
[0034] The method for accurate identification of Pythium soybean root rot resistance resources comprises the following steps:
[0035] (1) Activa...
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The invention provides a method for accurately identifying Pythium soybean root rot resistance resources. The method uses high-temperature sterilized rice grains to inoculate Pythium, then inoculates seeds of soybean germplasm resources with cultivated inoculum, and uses vermiculite as a substrate Cultivate in the greenhouse, investigate and classify the diseases of the plants and calculate the fresh weight ratio, and screen the resistance by performing variance analysis and cluster analysis on the plant fresh weight ratio (Ratio of weight) and disease index (Disease Index) and susceptibility resources, new accessions with resistance / tolerance to Pythium root rot were identified. The present invention determines the standard evaluation system for Pythium root rot resistance of soybean resources, optimizes the formula of the pathogen culture medium, creates a rice grain inoculum culture and inoculation method, and develops a disease index calculation method, which is simple, practical, efficient, and also It is suitable for identification research on the resistance of other soybean oomycete diseases.
Description
technical field [0001] The invention belongs to the field of research on the utilization of plant germplasm resources, and in particular relates to an accurate identification method for Pythium soybean root rot resistance resources. Background technique [0002] Soybean root rot (Soybean root rot) is a worldwide soil-borne disease that occurs relatively commonly in soybean planting areas at home and abroad. [0003] In recent years, Heilongjiang Province, the main soybean producing area in China, has been expanding the diseased area, and the disease is more serious in the northeast of Heilongjiang. Years of research have shown that soybean root rot pathogens mainly include Phytophthora spp., Fusarium spp. and Pythium spp. Pythium is a highly harmful soil-borne fungal disease. It has been confirmed internationally that more than 20 strains are pathogenic to soybeans. However, due to the low isolation ratio and similar pathogenic traits with other strains, there are few rese...
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IPC IPC(8): A01G7/06A01G22/40C12N1/14C12N1/04C12Q1/18C12R1/645
CPCA01G7/06A01G22/40C12N1/04C12N1/14C12Q1/18G01N2333/37
Inventor 毕影东来永才刘明李炜张劲松喻德跃刘淼王玲刘建新邸树峰杨光樊超刘凯栾晓燕侯国强夏天舒孙兵
Owner FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI