Control method of Spodoptera frugiperda

A technology for Spodoptera frugiperda and entomopathogenic nematodes, applied in the field of biological control, can solve problems such as damage and impact on crops, and achieve the effects of fast onset time, convenient application and strong ability to search for hosts

Inactive Publication Date: 2019-09-06
浙江绿神天敌生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The patent number is 201610164901.3, and the name of the invention is a method for controlling underground pests through entomopathogenic nematodes. This invention discloses a method for using entomopathogenic nematodes to control underground pests. However, when pests damage the leaves of crops, they will cause greater damage to crops. The invention does no...

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  • Control method of Spodoptera frugiperda
  • Control method of Spodoptera frugiperda

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Bioassay of entomopathogenic nematodes on young larvae of Spodoptera frugiperda

[0066] In a room temperature environment with a temperature of about 26°C, raise Spodoptera frugiperda larvae separately in a petri dish, put a piece of filter paper with a diameter of 9 cm in a glass petri dish with a diameter of 9 cm, and use fresh corn to feed Spodoptera frugiperda For stalks or young corn leaves, add 1ml of water to the filter paper every 24 hours to keep them moist, and raise them separately in petri dishes until the 2nd, 3rd, 4th, 5th or 6th instar larvae. Elute Steiner's nematode from the sponge block, dilute to 100IJs / ml, 250IJs / ml, 500IJs / ml concentration of nematode suspension with water, draw 1ml of entomopathogenic nematode suspension with a pipette gun, and evenly add it to the filter paper. Still add 1ml of clear water to the filter paper every day to keep moist, and observe and record the survival situation of the larvae of Spodoptera frugiperda at the same ...

Embodiment 2

[0070] Bioassay of Entomopathogenic Nematodes on Mature Larvae of Spodoptera frugiperda

[0071] In a room temperature environment with a temperature of about 26°C, the mature larvae of Spodoptera frugiperda were reared separately. After the larvae of Spodoptera frugiperda were raised to the 6th instar larvae, soil collected from the field was added to the lower cover of a 9 cm petri dish until the lower cover was flat. Qi, add appropriate amount of water to about 50% of the relative water content of the soil, put a piece of filter paper on the soil surface, spray about 2ml of clear water to keep it moist, put the 6th instar larvae and fresh corn stalks on the filter paper, put the same size Petri dish The lower cover is covered on the top, and the surroundings are sealed with parafilm to prevent escape. Fresh corn stalks were replaced every day until the mature larvae burrowed into the soil by themselves. Elute Heterobacterium nematodes from the sponge block, dilute to 100IJ...

Embodiment 3

[0075] Bioassays of Spodoptera frugiperda prepupa and pupae by entomopathogenic nematodes

[0076]In a room temperature environment with a temperature of about 26°C, the mature larvae of Spodoptera frugiperda were reared separately. After the larvae of Spodoptera frugiperda were raised to the 6th instar larvae, soil collected from the field was added to the lower cover of a 9 cm petri dish until the lower cover was flat. Qi, add appropriate amount of water to about 50% of the relative water content of the soil, put a piece of filter paper on the soil surface, spray about 2ml of clear water to keep it moist, put the 6th instar larvae and fresh corn stalks on the filter paper, put the same size Petri dish The lower cover is covered on the top, and the surroundings are sealed with parafilm to prevent escape. Replace fresh corn stalks every day until mature larvae drill into the soil by themselves. After 2 to 3 days, Spodoptera frugiperda develops into pre-pupation or pupae. Elut...

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Abstract

The invention discloses a control method of Spodoptera frugiperda. The control method comprises the following steps: step one, eluting entomopathogenetic nematodes from sponge blocks by using water toprepare entomopathogenetic nematode suspension liquid, wherein the entomopathogenetic nematode suspension liquid consists of water and entomopathogenetic nematodes; step two, uniformly applying the entomopathogenetic nematode suspension liquid obtained in the step one to leaf surfaces of crops and/or soil of the crops; step three, infecting the larvae of Spodoptera frugiperda on the leaf surfacesof crops and/or on the surface of soil by using entomopathogenetic nematodes, and infecting the aged larvae, prepupa and pupae of the Spodoptera frugiperda under the soil of the crops. According to the method, Spodoptera frugiperda is used for infecting leaf surface insects and underground insects of the Spodoptera frugiperda, biological control of the Spodoptera frugiperda is carried out, the environment is not polluted, drug resistance to the Spodoptera frugiperda is not generated, the Spodoptera frugiperda continuously proliferates in the environment, population can be established in the environment, and a controllable and preventable state caused by continuous and stable control of pests is achieved.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to a method for preventing and controlling Spodoptera frugiperda. Background technique [0002] Fall armyworm is a major pest of corn, sorghum, sugarcane and other food and economic crops. The Food and Agriculture Organization of the United Nations issued a global warning in 2017. Since the insect invaded my country's Yunnan Province in January 2019, it is gradually moving north and approaching China's main corn-producing areas. [0003] Nematodes are the most abundant soil invertebrates in the soil, most of which live in the rhizosphere of plants. According to feeding habits, they can be divided into three categories: saprophytic nematodes, plant parasitic nematodes and animal parasitic nematodes. Entomopathogenic nematodes obligately parasitize insects, and more than 1,000 species are known. Entomopathogenic nematodes move through the water film to find the host inse...

Claims

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Application Information

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IPC IPC(8): A01G13/00
CPCA01G13/00
Inventor 盛锡良徐彩霞孙文明孔令文陶巧静
Owner 浙江绿神天敌生物技术有限公司
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