Label-free live cell detection microfluidic chip based on quasi-Bessel optical waveguide structure

Abstract

The invention discloses a marker-free live cell detection micro-fluidic chip based on a quasi-bessel optical waveguide structure. An incident optical fiber coupling waveguide structure and a quasi-bessel light beam regulation and control waveguide structure are sequentially arranged in a coaxial way; a marker-free scattering receiving waveguide structure and a collection optical fiber positioning structure are sequentially in a coaxial way; the included angle of the axial line of the incident optical fiber coupling waveguide structure and the quasi-bessel light beam regulation and control waveguide structure and the axial line of the marker-free scattering receiving waveguide structure and the collection optical fiber positioning structure is 6 to 9 degrees. The front end of the micro-fluidic chip realizes the quasi-bessel light beam waveguide structure which has the self restoration shaping capability and cannot easily generate diffraction; the problem of incident light beam scattering angle control is solved; the signal-to-noise ratio of the cell scattering forward direction small-angle important light information in the micro-fluidic chip is improved; the marker-free scattering receiving waveguide structure is designed at the receiving end of the microfluidic chip; the scattering signal in a certain angle can be precisely guided out and focused; the high-precision microfluidics cell recognition and classification in the microfluidic system is realized.

Description

technical field [0001] The invention belongs to the research field of label-free cell scattering detection, and relates to a label-free living cell detection microfluidic chip based on a quasi-Bessel optical waveguide structure. Background technique [0002] Microfluidic chip is an important tool for cell detection, widely used in clinical blood diagnosis, immunobiology, etc. At present, conventional microfluidic cell detection technologies mostly use fluorescent labeling methods. Due to the toxicity of fluorescent dyes and other issues, it has irreversible effects on cell viability, biological function and state, and cannot meet the needs of subsequent research and application such as drug sensitivity experiments and disease state tracking. Therefore, label-free cell-to-scatter detection methods based on microfluidic systems have become a research hotspot in recent years. The optical scattering information of cells contains a large number of biological and physical charac...

AI Technical Summary

Problems solved by technology

However, the scattering signal of cells is extremely weak and is easily interfered by external noise

Leakage of divergent incident beams is the main problem that causes the signal-to-noise ratio of cells to scatter to drop significantly, and even to be submerged

The incident beam of the currently disclosed marker-free scattering measurement mainly adopts the spatial linear beam modulation method, and the control effect of the divergence angle of the incident beam is poor, especially when the optical fiber system is used for measurement, it is very easy to cause the scattering signal to be submerged

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