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Stropharia rugoso-annulata polysaccharide, and preparation method and application thereof

The technology of the polysaccharide and the polysaccharide is applied in the field of the polysaccharide and the preparation of the polysaccharide. The effect of enhancing phagocytic activity

Active Publication Date: 2019-09-20
CHINA WEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the fine structure, immunomodulatory activity and application of Stropharia polysaccharides

Method used

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  • Stropharia rugoso-annulata polysaccharide, and preparation method and application thereof
  • Stropharia rugoso-annulata polysaccharide, and preparation method and application thereof
  • Stropharia rugoso-annulata polysaccharide, and preparation method and application thereof

Examples

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Embodiment 1

[0054] Example 1 Separation and extraction of Stropharia polysaccharide SR-1

[0055] 1.1 Separation and extraction of Stropharia polysaccharide SR-1

[0056] 1.1.1 Water Extraction and Alcohol Precipitation Method to Extract Crude Polysaccharide from Stropharia grandis

[0057] The pulverized Stropharia fruiting body powder and distilled water were boiled in a water bath at 100°C for 3 hours at a solid-liquid ratio (W / V) of 1:3, boiled 3 times, and the supernatant was collected and concentrated to 200 mL. Three times the volume of absolute ethanol was added to form a flocculent precipitate, which was collected and dried. The protein in the extract was removed by Sevage method to obtain the crude polysaccharide of Stropharia grandis.

[0058] 1.1.2 Separation and purification of crude polysaccharide from Stropharia grandis by DEAE-cellulose column chromatography

[0059]Weigh 50.00 g of DEAE-cellulose, stir evenly with distilled water, and soak for 24 hours to remove suspen...

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Abstract

The invention discloses stropharia rugoso-annulata polysaccharide, and a preparation method and application thereof. The polysaccharide is prepared by sequentially carrying out water extraction and alcohol precipitation, protein removal, ion-exchange column chromatography and dialysis concentration on stropharia rugoso-annulata fruit bodies. The stropharia rugoso-annulata polysaccharide is a heteropolysaccharide composed of alpha-D-galactose, alpha-D-glucose and beta-D-glucose, wherein the molar ratio of residues of alpha-D-galactose to residues of glucose (including the alpha-D-glucose and the beta-D-glucose) is 3:1, and the molar ratio of residues of the alpha-D-glucose to the residues of the beta-D-glucose is 2:1. The polysaccharide has remarkable immunoregulatory activity.

Description

technical field [0001] The invention belongs to the technical field of fungal polysaccharide applications, and in particular relates to a stropharia polysaccharide and its preparation method and application. Background technique [0002] Polysaccharides are a class of biomacromolecules ubiquitous in organisms. Modern biochemical studies have found that polysaccharides can participate in the cytoskeleton and some bioactive molecules; such as cellulose and chitin that maintain cell structure; starch and glycogen that provide energy sources. The development of polysaccharides is late, and the structure is complex and diverse, so it is difficult to study its structure and activity. [0003] In the past 20 years, research reports on the biological activities of polysaccharides have mainly focused on immune regulation, anti-tumor, anti-virus, anti-oxidation, and hypoglycemic effects, and their effects are multi-path, multi-link, and multi-target. [0004] Stropharia rugosoannula...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04A61P35/00A23L33/125
CPCC08B37/006A61P37/04A61P35/00A23L33/125A23V2002/00A23V2200/324A23V2200/308
Inventor 侯怡铃丁祥蒋琳
Owner CHINA WEST NORMAL UNIVERSITY
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