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CircRNA relevant to prostatic cancers and application of circRNA

A prostate cancer and product technology, applied in circRNA and its application fields, can solve the problems of lack of accurate treatment of etiology, unknown pathogenesis of CRPC, etc., and achieve the effect of precise treatment

Active Publication Date: 2019-09-24
天津市泌尿外科研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the pathogenesis of CRPC is still unknown, there is a lack of precise treatment for the etiology in clinical practice, which is a difficulty and hot spot in current urology research

Method used

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  • CircRNA relevant to prostatic cancers and application of circRNA
  • CircRNA relevant to prostatic cancers and application of circRNA
  • CircRNA relevant to prostatic cancers and application of circRNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Design and verification of primers for circRNA

[0042] 1. Design of circRNA primers

[0043] Download the fasta format sequence of has_circ_0092339 on circBase. The first and last two bases of the obtained original sequence are indicated with a special color. Cut any point in the original sequence, cut all the points from this point to the end of the sequence, and then move them to the front of the original sequence together to form a new sequence. The first and last clips of the original sequence become two consecutive bases in the new sequence, forming a back-splicing site. Design primers for novel sequences. Among them, the back primer requires spanning the reverse cleavage site. The primers designed here are back-to-back primers, and the back-to-back primer sequences are shown in SEQ ID NO.1-2; find the transcript of the circRNA in circBase, and design primers for the transcript. This primer is called a face-to-face primer, and the face-to-face primer...

Embodiment 2

[0056] Example 2 Verification of circRNA in patient tissue

[0057] 1. Collection of samples

[0058] Tissue samples of prostate cancer patients admitted to our hospital were collected, including 5 cases in each group of ADPC and CRPC, excluding those with a history of mental illness, abnormal cognitive and understanding functions, suffering from other malignant tumors, and those who could not undergo radical surgery due to special reasons patients with sex resection.

[0059] 2. Grinding of patient tissue homogenate

[0060] Take out the patient's tissue from the -80°C refrigerator, cut the size of mung bean, put it into the grinding rod immediately, add 1ml of normal saline mixed with ice water, and grind it repeatedly with the grinding rod until it becomes a homogenate. Draw 1ml homogenate, add 1ml trizol, shake vigorously, and mix well. Then incubate on ice for 5min to make 2ml premix

[0061] 3. Extraction of total RNA from patient tissue

[0062] Pipette 1ml of the ...

Embodiment 3

[0067] Example 3 Base Scope Verification of Patient Tissue

[0068] 1. Patient tissue paraffin section

[0069] Soak the excised tissue in 10% neutral formalin fixative solution at room temperature for 16-32h. Samples were then washed with 1×PBS. Dehydration was performed with a gradient of standard ethanol and finally xylene. Paraffin embedding was performed conventionally. Adjust the slice thickness to 4 μm for tissue sectioning. Place the excised paraffin sections containing tissues in a 40-45°C water bath. Pick up the slices with a glass slide, being careful not to overlap. Dry at room temperature overnight.

[0070] 2. HE staining

[0071]Before performing Base Scope, HE staining should be performed to confirm the position. Firstly, it is dried in an electric oven at 75°C for 1 hour, and then dewaxed in cylinders. The corresponding organic solvents and time are shown in Table 1, and the order in the table is the order of passing through the tank.

[0072] Table ...

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Abstract

The invention discloses a circRNA relevant to prostatic cancers and application of the circRNA. The circRNA is hsa_circ_0092339. According to the circRNA relevant to prostatic cancers and the application of the circRNA, it is found for the first time that the expression of the hsa_circ_0092339 in castration-resistant prostate cancers is up-regulated, and by detecting the level of the hsa_circ_0092339, whether a testee suffers from castration-resistant prostate cancers or not can be judged.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a circRNA related to prostate cancer and its application. Background technique [0002] Prostate cancer is a common malignancy and the second leading cause of cancer death in men. In recent years, the incidence of prostate cancer in my country has shown a rapid upward trend year by year, from 5.8 / 100,000 in 2004 to 8 / 100,000 in 2009. The absolute value of the incidence rate increased the fastest in Hong Kong, China, from 16.5 / 100,000 in 1999 to 28.1 / 100,000 in 2010. In Taiwan and Shanghai, prostate cancer has ranked the fifth most common tumor in men and the first among urinary system tumors. Due to the relatively backward screening of prostate cancer susceptible population in my country, advanced prostate cancer accounts for about 70% of the first diagnosed prostate cancer; the proportion of newly diagnosed advanced prostate cancer in my country is much higher than that in European an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 尚芝群牛远杰李翰林张博雅
Owner 天津市泌尿外科研究所
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