Supercharge Your Innovation With Domain-Expert AI Agents!

Fermentation cultivation method of eel-source bacteroides fragilis

A technology of fermentation and cultivation of Bacteroides fragilis, which is applied in the field of fermentation and cultivation of Bacteroides fragilis derived from eels, can solve the problems of low viable bacteria, waste costs, aging of Bacteroides fragilis, etc., and achieve the effect of simple process and pollution reduction

Pending Publication Date: 2019-09-27
JIMEI UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After centrifugation, B. fragilis ages, resulting in low viable counts
The whole operation process is time-consuming and labor-intensive, and the process consumes a large amount of culture medium, which also causes waste and high cost to a certain extent.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Isolation and identification of Bacteroides fragilis from eels:

[0033] (1) Preparation of intestinal samples: fresh eels (taken from the marine farm of Xiamen Jimei University Fisheries College) were taken out from the incubator, after anesthetized with a certain dose of MS-222, the fish body surface was wiped with 75% alcohol and removed. Place it on a clean bench. Use sterile dissecting scissors to cut the eel body from the anus to the eel's mouth, and place the eel intestine in a clean dissection plate, remove the fat and blood around the intestine, cut the intestine with sterile scissors, and use sterile dissection Gently scrape the intestinal contents (including the intestinal mucosa and intestinal contents) with a knife, put them into a sterile centrifuge tube, wash the inner wall of the intestinal tract with sterilized PBS buffer solution, and mix the washing solution with the scraped contents. Mix the contents and vortex thoroughly. Pipette 0.1 mL from th...

Embodiment 2

[0049] 2. The fermentation and cultivation method of eel-derived Bacteroides fragilis, the steps are as follows:

[0050] In step 2), the proliferation culture time is 12 hours;

[0051] In step 3), inoculate in a 250mL conical flask with 2% inoculum amount (volume ratio), the liquid volume in the conical flask is 100mL, and the pH is 8.0; the conical flask is placed in a constant temperature incubator at 28°C for static cultivation;

[0052] All the other steps are with embodiment 1;

[0053] After standing at constant temperature for 24 hours, the cell concentration was measured with a hemocytometer, and the concentration of Bacteroides fragilis reached the maximum value of 5.1×10 8 individual / mL. Take 10 mL of fermented Bacteroides fragilis bacteria liquid, set up 3 repetitions, and confirm that the viable bacteria content of Bacteroides fragilis in the fermentation culture is 4.51×10 8 CFU / mL.

Embodiment 3

[0055] 2. The fermentation and cultivation method of eel-derived Bacteroides fragilis, the steps are as follows:

[0056] In step 2), the proliferation culture time is 14 hours;

[0057] In step 3), inoculate in a 250mL Erlenmeyer flask with 6% inoculum amount (volume ratio), the liquid volume of the Erlenmeyer flask is 100mL, and the pH is 9.0; the Erlenmeyer flask is placed in a constant temperature incubator at 37°C for static cultivation;

[0058] All the other steps are with embodiment 1;

[0059] After standing at constant temperature for 24 hours, the cell concentration was measured with a hemocytometer, and the concentration of Bacteroides fragilis reached the maximum value of 4.3×10 8 individual / mL. Take 10 mL of fermented Bacteroides fragilis bacteria liquid, set up 3 repetitions, and confirm that the viable bacteria content of Bacteroides fragilis in the fermentation culture is 3.4×10 8 CFU / mL.

[0060] In the present invention, the activated Bacteroides fragili...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fermentation cultivation method of eel-source bacteroides fragilis. The preparation method comprises the following steps: taking eel-source bacteroides fragilis to be inoculated into an anaerobic tube loaded with a seed activating culture medium, and carrying out static anaerobic activating culture for 24 hours under a temperature of 25-38 DEG C; enabling an activated cultured bacteria solution to be connected into the anaerobic tube loaded with an enrichment medium, then carrying out enrichment culture for 9 to 14 hours under a temperature of 25 to 38 DEG C and under a condition of 25 to 38 DEG C and an inoculum size of 2 percent, so as to obtain an enrichment cultured bacteria solution; enabling the enrichment cultured bacteria solution to be inoculated according to the inoculum size of 2-6 percent into a triangular flask containing a fermentation medium, wherein the pH value of the solution in the triangular flask is 7 to 9, and putting the triangular flask into a constant temperature incubator with a temperature of 25 to 37 DEG C. The bacteroides fragilis obtained by using the fermentation cultivation method has high concentration and high viable bacteria quantity, the operation process is simple, pollution caused to bacteroides fragilis also can be reduced during a cultivation process, the time is saved, and the culture medium waste can be reduced.

Description

【Technical field】 [0001] The invention relates to the field of microbial fermentation and cultivation, in particular to a method for fermentation and cultivation of eel-derived Bacteroides fragilis. 【Background technique】 [0002] The European eel is native to the Atlantic Ocean. It lives in rivers, estuaries, and lagoons. It is a carnivore that likes to drill holes and live in burrows. It lives on shrimp, crabs, shellfish, and sea worms. In 1993, mainland China introduced 20 tons of European eel fry for trial breeding. my country is the world's largest producer of eels. As early as 2003, the annual output of eels had reached 178,170 tons, ranking first in the world. Therefore, eel aquaculture occupies a pivotal position in the entire aquatic industry. [0003] Bacteroides fragilis (Bacteroides fragilis), as a type species of Bacteroides (enterotoxigenic and non-enterotoxigenic), normally colonizes the intestinal tract of mammals. With the deepening of its research, schola...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 林茂吴少坤江兴龙翟少伟
Owner JIMEI UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com