Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ovum collection method and preparation used in bovine living body superovulation

A technique for in vivo egg collection and preparation, which is applied to the improved field of cattle in vivo superovulation egg collection, the preparation of cattle in vivo superovulation egg collection, and the improved field of cattle in vivo egg collection, can solve the problem that the fertilization rate and the cleavage rate are not significantly different, It is difficult to effectively increase the number of oocytes obtained and the blastocyst rate is low.

Active Publication Date: 2022-06-07
天津力牧生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. In vitro culture system: Revel et al. studied the in vitro maturation, in vitro fertilization and in vitro culture of 3-month-old calf oocytes, and found that the fertilization rate and cleavage rate were not significantly different from those of adult cattle. However, in vitro culture After 7 days, the blastocyst rate was significantly lower than that of adult cattle, and the pregnancy rate was also significantly lower than that of adult cattle, which indicated that some key factors controlling blastocyst formation in calf oocytes may not have fully appeared or expressed
[0013] However, there are still some deficiencies in the existing methods, especially the difficulty in effectively increasing the number of oocytes obtained

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ovum collection method and preparation used in bovine living body superovulation
  • Ovum collection method and preparation used in bovine living body superovulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Cattle live egg collection

[0063] 1. Superovulation treatment before egg retrieval

[0064] The cows with the number of follicles ≥ 20 with a diameter of less than 8 mm on both ovaries were screened as oocyte donor cows, and the cows were 2 to 5 years old; FSH preparations were injected in 4 equal doses within two days for superovulation, and each injection was performed. 12h interval, the dose of each injection is 0.5μg / kg bovine body weight in terms of FSH;

[0065] 2. Live egg collection

[0066] 48 hours after the last injection of the FSH preparation, in vivo egg collection was performed. The operation of in vivo egg collection was as follows:

[0067] (21) Preoperative preparation: adjust the equipment to the working state (the pressure of the egg suction vacuum pump is 50-90mmHg), inject about 5ml of egg suction liquid into the collection tube, place it in a thermostat, and adjust the temperature of the thermostat to 39°C; The transparent plastic...

Embodiment 2

[0074] Example 2: Cattle live egg collection

[0075] 1. Superovulation treatment before oocyte retrieval: each injection dose is calculated as 0.4 μg / kg bovine body weight in FSH, and the rest are treated with embodiment 1;

[0076] 2, live egg collection: handle with embodiment 1;

[0077] 3. Detection of oocytes: the same treatment as in Example 1.

[0078] FSH preparation: bovine follicle-stimulating hormone (50 μg / ml), nilestriol (FSH:nilestriol=100:15), poloxamer 188 (concentration 0.5 mg / ml).

[0079] Donor cattle: Holstein (dairy cattle breed), 11 heads.

Embodiment 3

[0080] Example 3: Cattle live egg collection

[0081] 1. Superovulation treatment before oocyte retrieval: each injection dose is calculated as 0.6 μg / kg bovine body weight in FSH, and the rest are treated with embodiment 1;

[0082] 2, live egg collection: handle with embodiment 1;

[0083] 3. Detection of oocytes: the same treatment as in Example 1.

[0084] FSH preparation: bovine follicle-stimulating hormone (25 μg / ml), nilestriol (FSH:nilestriol=100:25), poloxamer 188 (concentration 0.3 mg / ml).

[0085] Donor cattle: Simmental (beef cattle breed), 9 heads.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for ovum collection by superovulation in living bovines and a used preparation. Specifically, in one aspect, the present invention relates to the use of FSH in preparing a preparation for bovine ovum collection, and the preparation is an aqueous solution containing bovine follicle-stimulating hormone at a concentration of 25-50 μg / ml. In another aspect, the present invention relates to a FSH preparation, which is an aqueous solution containing bovine follicle-stimulating hormone at a concentration of 25-50 μg / ml. Said bovine live egg collection comprises the following steps: superovulation treatment before egg collection, live egg collection, and detection of oocytes. Using the method of the invention to collect live bovine eggs has excellent technical effects as described in the specification. For example, compared with conventional methods, the method of the invention can obtain a large proportion of usable oocytes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an improved method for collecting live bovine eggs, in particular to an improved method for collecting live bovine eggs by means of the method of the invention, whereby a significantly larger number of oocytes can be obtained. The present invention also relates to a preparation for in vivo superovulation of bovine eggs, comprising FSH. Background technique [0002] In vivo superovulation, also known as superovulation, superovulation, superovulation, etc., has been used in the animal husbandry industry for decades. Its basic principle is to inject FSH (also known as follicle-stimulating hormone in the art , follicle-stimulating hormone, follicle-stimulating hormone, etc.) to promote the excessive discharge of oocytes from the ovary, thereby improving the efficiency of animal breeding. Superovulation technology is a technology that uses exogenous hormones to stimulate animals to release...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075A61K38/24A61P15/08
CPCC12N5/0609A61K38/24A61P15/08C12N2509/00
Inventor 王小武郭晶王娜郝少强赵明礼张月桥杨尚斌何伟马毅郭春明
Owner 天津力牧生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com