Method for culturing and preparing cordyceps militaris in egg

A technology for Cordyceps militaris and eggs is applied in the field of culture and preparation of Cordyceps militaris in eggs, and can solve the problems of complex culturing process steps, long culturing period, limited nutrients and the like

Active Publication Date: 2019-10-01
ZHOUKOU VOCATIONAL & TECHN COLLEGE +2
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Traditional culture substrates for Cordyceps militaris are mainly rice, wheat, sorghum, silkworm chrysalis powder, etc. These types of culture substrates have limited nutrients and need to add a variety of auxiliary materials, and the traditional cultivation process is complicated and the cultivation period is long

Method used

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  • Method for culturing and preparing cordyceps militaris in egg
  • Method for culturing and preparing cordyceps militaris in egg
  • Method for culturing and preparing cordyceps militaris in egg

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Embodiment 1

[0028] A method for cultivating Cordyceps militaris in eggs, characterized in that it comprises the following steps:

[0029] (1) Compatibility and optimization of solid and liquid medium: solid medium consists of 200 grams of potatoes, 20 grams of glucose, 2.5 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 20 grams of agar, and 1000 ml of pure water. The pH is 7; the liquid medium is composed of 20 grams of glucose, 20 grams of agar, 10 grams of peptone, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, and 1000 milliliters of ultrapure water. The pH of the medium is 7;

[0030] (2) Screening, optimization, rejuvenation and preservation of strains: Streak the strains of Cordyceps militaris on the slant of solid medium, and culture them in the incubator at 28°C for 7 days to obtain activated strains of Cordyceps militaris, and re- Select, pick out the colonies and inoculate them on the solid medium, and continue to cultivate t...

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Abstract

The invention provides a method for culturing and preparing cordyceps militaris in an egg, and relates to the technical field of fungus culture. Solid and liquid culture mediums are optimized, a cordyceps militaris strain is bred, the cordyceps militaris strain is inoculated into the allantoic cavity and yolk sac cavity of a pollution-free egg according to a certain dosage to be cultured for a certain period of time under the proper temperature, humidity and dark environment, the mycelium growth optimum production conditions of the cordyceps militaris strain in the egg are studied according tothe inoculation dosage, part and time, the proper culture conditions and preparing method are determined, and the egg powder with cordyceps militaris is prepared and collected through the processes of disinfecting, smashing, pulping, filtering and then spray-drying. The technological method is simple, convenient to implement, controllable and stable and provides the technological basis and reference for industrial production of cordyceps militaris.

Description

technical field [0001] The invention relates to the technical field of fungal culture, in particular to a method for cultivating and preparing Cordyceps militaris in eggs. Background technique [0002] With the occurrence of mixed infection of livestock and poultry infectious diseases, subclinical diseases, chronic diseases, immunosuppressive and tumor diseases, immune enhancers have become one of the most active researches in the field of applied medicine. Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, is similar to the active ingredients, nutritional value and pharmacological effects of Cordyceps sinensis. It has the activity of promoting macrophages and natural killer cells, regulating humoral immunity and cellular immunity, anti-tumor, protecting liver and kidney , sedation and other effects, the study of it as an immune enhancer has attracted people's attention day by day. At present, there are many medicines, health food and beauty pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 赵庆枫朱平军袁文菊杜海霞董凤华陈勇臻丁玉陈鹏举王栋
Owner ZHOUKOU VOCATIONAL & TECHN COLLEGE
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