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Microbial compositions and methods

A composition, microbiological technology, applied in the field of treatment of plants, plant parts or soil, capable of solving problems affecting plants, inconsistent performance, small crop benefits, etc.

Active Publication Date: 2019-10-01
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, microorganisms can also negatively affect plants in some cases, and existing microbial products sometimes exhibit inconsistent performance or minimal crop benefit

Method used

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  • Microbial compositions and methods
  • Microbial compositions and methods
  • Microbial compositions and methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0157] Example 1. Origin and isolation of MON 201510

[0158] Microbial strain MON 201510 was isolated from the rhizosphere of crop plants grown in a controlled environment using soil obtained from a native grassland in Iowa, USA. By serial dilution into R2A medium (0.5g casamino acids, 0.5g dextrose, 0.5g peptone, 0.5g soluble starch, 0.5g yeast extract, 0.5g special sodium, 0.3g K 2 HPO 4 , 0.05gMgSO 4 ·7H 2 0, 15 g of agar and water in a final volume of 1000 mL) to isolate strains from the collected samples. Individual colonies formed on the medium surface from the sonicated cell slurries were picked for further analysis.

Embodiment 2

[0159] Example 2. Identification and sequencing of MON 201510

[0160] The MON 201510 isolate was identified as Pseudomonas chlorospinum by 16S ribosomal DNA (rDNA) sequencing. The 16S rDNA sequence was determined by genome-wide next-generation sequencing (NGS) and derivation of related 16S rDNA sequences. The 16S rDNA sequence of the MON 201510 isolate is provided as SEQ ID NO:1.

Embodiment 3

[0161] Example 3. Growth chamber assay for controlling sudden soybean death syndrome (SDS) on MON 201510

[0162] The isolated MON 201510 strain was grown in liquid culture and then frozen in 15% glycerol before use. Soybean seeds were broth-treated with a thawed broth suspension of the MON 201510 isolate at the time of sowing and evaluated in four studies in a growth chamber inoculation disease experiment. The same soybean variety was used for all experiments (maturation group 3.4). Experimental sites were inoculated immediately before planting using sterilized sorghum pellets that had been previously colonized with the SDS pathogen Fusarium cladoides. Control plants were left untreated and inoculated. Disease assessment was performed five weeks after inoculation using an ordinal scale (1-6), where 1 corresponds to asymptomatic plants and 5 corresponds to fully chlorotic and / or necrotic plants. A rating of 6 indicates failure to sprout.

[0163] All experiments utilized a...

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Abstract

Microbial compositions for application to plants, plant parts and plant seeds are provided for improvement of plant disease resistance and / or other beneficial plant traits, particularly resistance ortolerance to soybean sudden death syndrome (SDS) caused by a Fusarium species and / or disease caused by a Pythium species. Methods of making and applying microbial compositions or formulations to plants, plant parts or plant seeds or to growth media are further provided to increase or improve plant disease resistance and / or other beneficial plant traits.

Description

[0001] References to related applications [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 441,918, filed on January 3, 2017, and U.S. Provisional Application No. 62 / 449,974, filed on January 24, 2017, each of which is incorporated herein by reference in its entirety. middle. [0003] Incorporation of sequence listings [0004] The sequence listing contained in the file entitled "MONS407WO_ST25.txt", which is 3 kilobytes as measured in a Microsoft Windows operating system and was created on January 2, 2018. Technical field [0005] The present disclosure relates to compositions containing microorganisms for protecting plants from infection caused by one or more plant pathogens and / or reducing the severity or symptoms of diseases caused by one or more plant pathogens, and use of those combinations Treatment of plants, plant parts or soil. Background technique [0006] Some microorganisms, including bacteria and fungi, can positively affe...

Claims

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Application Information

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IPC IPC(8): A01C1/06A01N63/02C12R1/38A01N63/27
CPCA01N63/27C12R2001/38C12N1/205A01N63/22A01N63/36A01N63/38A01C1/06C12N15/8205
Inventor M·M·D·阿里亚斯K·L·费雷拉C·J·格兰德利克L·L·路非亚R·T·麦卡恩S·R·谢彻J·M·斯坦
Owner MONSANTO TECH LLC
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