Mutation breeding method for Chinese pennisetum

A technology of mutation breeding and pennisetum, applied in the field of breeding, can solve the problems of shortage of ornamental pennisetum species and failure to meet market demand, and achieve the effects of accelerating the process of breeding, easy promotion, and mature methods

Active Publication Date: 2019-10-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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Problems solved by technology

Pennisetum is widely used as an ornamental plant in major urban parks and country parks in my country. In the northern region, with the development of low-maintenance and water-saving gardens, the demand for Pennisetum is gradually increasing; however, ornamental Pennisetum in my country Variety shortage, unable to meet market demand

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  • Mutation breeding method for Chinese pennisetum
  • Mutation breeding method for Chinese pennisetum
  • Mutation breeding method for Chinese pennisetum

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Embodiment 1

[0033] Example 1 Breeding Pennisetum D41

[0034] Select the plump seeds of the ornamental plant 'Long Spike' pennisetum and sterilize with 5% NaClO solution for 15-20 minutes, then wash with distilled water for 3-4 times, and finally soak the seeds in distilled water for 10 hours. Then, the sterilized seeds were transferred to a 250ml Erlenmeyer flask filled with 50ml of 1% EMS solution, shaken at room temperature (26±1)°C for 4h in the dark, and the shaker speed was 120rmp / min. Wherein, the EMS solution is prepared by 0.5mM phosphate buffer (pH 7.0). After the treatment, the treated seeds were shaken and washed with distilled water for 4 hours under the above conditions, during which the water was changed 5 times. After washing, the treated seeds were sown on a petri dish covered with three layers of filter paper to germinate under the conditions of a light culture room (temperature (26±1)°C, light intensity 2500-3000Lux, photoperiod 14h light / 10h dark) . After 10 days of...

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Abstract

The invention provides a mutation breeding method for Chinese pennisetum, and belongs to the technical field of seed selection of new-variety plants. The method comprises the steps that Chinese pennisetum seeds are disinfected, soaked, inoculated into an EMS solution with the volume percent content of 0.4%-1.2% and induced for 4-6 hours under the indoor-temperature dark condition, the induced seeds are planted, and after the steps of seed germination, seedling culturing in a greenhouse, transplanting into large land and the like are conducted, in combination with field investigation, good single plants are screened out; a tillering cuttage method is adopted for asexual propagation expansion for the selected single plants, so that good characters of the selected single plants are fixed. Through continuous 4-6 generation asexual propagation expansion and three years of variety comparison tests, stable and consistent new-strain Chinese pennisetum can be cultured. The method is simple andconvenient to implement, mature, stable and easy to popularize and accelerates the process of culturing the new-variety ornamental Chinese pennisetum.

Description

technical field [0001] The invention relates to breeding technology, in particular to a pennisetum induced breeding method. Background technique [0002] Ethyl methanesulfonate (EMS) is a commonly used and very effective chemical mutagen, which can induce high-density serial allelic point mutations, and the mutants produced by mutagenesis have rich phenotypes, Good stability. EMS mutagenesis is one of the important methods for plant germplasm innovation and new variety breeding, and has been widely used in field crops and garden plant breeding. [0003] As an ornamental plant, Pennisetum is widely used at home and abroad. This plant has strong drought resistance and barren resistance, and has a beautiful plant shape and beautiful inflorescences, and has very good ornamental value. Pennisetum is widely used as an ornamental plant in major urban parks and country parks in my country. In the northern region, with the development of low-maintenance and water-saving gardens, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01G22/00
CPCA01G22/00A01H1/06
Inventor 岳跃森范希峰武菊英滕珂张辉韩朝腾文军温海峰
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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