Mutation breeding method for Chinese pennisetum
A technology of mutation breeding and pennisetum, applied in the field of breeding, can solve the problems of shortage of ornamental pennisetum species and failure to meet market demand, and achieve the effects of accelerating the process of breeding, easy promotion, and mature methods
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[0033] Example 1 Breeding Pennisetum D41
[0034] Select the plump seeds of the ornamental plant 'Long Spike' pennisetum and sterilize with 5% NaClO solution for 15-20 minutes, then wash with distilled water for 3-4 times, and finally soak the seeds in distilled water for 10 hours. Then, the sterilized seeds were transferred to a 250ml Erlenmeyer flask filled with 50ml of 1% EMS solution, shaken at room temperature (26±1)°C for 4h in the dark, and the shaker speed was 120rmp / min. Wherein, the EMS solution is prepared by 0.5mM phosphate buffer (pH 7.0). After the treatment, the treated seeds were shaken and washed with distilled water for 4 hours under the above conditions, during which the water was changed 5 times. After washing, the treated seeds were sown on a petri dish covered with three layers of filter paper to germinate under the conditions of a light culture room (temperature (26±1)°C, light intensity 2500-3000Lux, photoperiod 14h light / 10h dark) . After 10 days of...
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