Method for tissue culture of Xungen tea, making method and rolling device

A technology of tissue culture and rolling device, which is applied in horticultural methods, botanical equipment and methods, tea treatment before extraction, etc. It can solve the problems of tea filtering, pouring and cleaning complex, so as to achieve ornamental value, improve quality and ensure yield Effect

Pending Publication Date: 2019-10-18
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the brewing process, the filtering, pouring and cleaning of tea leaves are relatively complicated.

Method used

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  • Method for tissue culture of Xungen tea, making method and rolling device
  • Method for tissue culture of Xungen tea, making method and rolling device
  • Method for tissue culture of Xungen tea, making method and rolling device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: a kind of method for root-seeking tea tissue culture, comprising:

[0033]S1, select the green tender stem tip of the original tea tree plant, the length is 1-10cm; the original tea tree resources include all species and varieties of Camellia plants that can be used for drinking tea production, specifically include the following strains: Wubao tea series: Guangxi tea, Dabao tea, Guangnan tea, Wushi tea, and sparse tooth tea. Five-column tea series: Thick shaft tea, five-column tea, old black tea, Dali tea, Burmese tea, Yuanji tea, wrinkled leaf tea, Maguan tea, Hani tea, multi-petal tea. Tufang tea series: Mengla tea, Dehong tea, Tuli tea, quasi-xie tea, false Tufang tea, Rongjiang tea, purple fruit tea, Duomai tea. Tea series: tea, bitter tea (variety), white hair tea (variety), Pu'er tea, multi-calyx tea, quasi-fine-calyx tea, Yuanjiang tea, high tree tea. And group species: Mengku big-leaf tea, Menghai big-leaf tea, Fengqing big-leaf tea (the above ar...

no. 1 example

[0048] The first example: In March 2019, 56 green young stem tips were collected from the individual tea garden in Laoshan, Qingdao, the most northern tea garden in my country, with a length of 1-10cm (such as 1cm, 2cm, 3.5cm, 10cm, etc.), First, rinse with running water for 1 hour on a sterile workbench. Under aseptic conditions, they were sterilized with 75% alcohol for 30 seconds, 0.1% mercuric chloride for 6 minutes, and rinsed with sterile water for 5 times. Then be divided into 1.0-1.5cm (as 1.0cm, 1.1cm, 1.5cm etc.) banded stem segment or shoot tip, inoculate in MS+6-BA2.0mg / L+NAA0.1mg / L medium, 30 days to obtain sterile in vitro shoots.

[0049] Further, the aseptic in vitro shoots are cut into 1.0-1.5cm (such as 1.0cm, 1.1cm, 1.5cm, etc.) shoot tip or segmented stem section, and transferred to medium MS+6-BA3 .0mg / L+IBA0.1mg / L for proliferation culture. On day 15, isolated clustered buds (average 3.2 buds each) were obtained. Then, the clustered buds were separated...

no. 2 example

[0057] The second example: the production of Biluochun root-seeking in Yixing, Jiangsu

[0058] In March 2019, 100 green young stem tips were collected from individual tea gardens in Yixing, Jiangsu, with a length of 1-6cm. After collection, they were delivered to the laboratory in a fresh mode by SF Express.

[0059] The primary culture was first washed with running water on a sterile bench for 2 hours, then sterilized with 75% alcohol for 60 seconds, 0.1% mercury liter for 15 minutes, and rinsed with sterile water for 10 times under aseptic conditions. Then it is divided into 0.5.0-1.2cm (such as 0.5cm, 1.0cm, 1.1cm, 1.2cm, etc.) with jointed stem segments or shoot tips, inoculated in MS+6-BA3.0mg / L+NAA0.1mg / In L medium, sterile in vitro shoots were obtained in 15 days.

[0060] Further, the aseptic isolated shoots are cut into shoot tips or sectioned stems with a length of 0.5-1.2cm (such as 0.5cm, 1.0cm, 1.1cm, 1.2cm, etc.), and transferred to the medium MS+ Proliferati...

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Abstract

The invention discloses a method for tissue culture of Xungen tea to obtain tissue culture seedlings of tea trees with roots and a making method for the Xungen tea. The making method is used for making the Xungen tea through the tissue culture seedlings of the tea trees with the roots in combination with a rolling device or manual rolling and other processes. The Xungen tea can be planted indoorsin a three-dimensional, multi-layered and continuous manner, and the yield of the Xungen tea is ensured; the fresh beauty of the mini plants of the tea trees can be fully displayed in the brewing process, the ornamental property is better, and the quality of tea drinking is significantly improved; the Xungen tea has active ingredients of roots such as stachyose and raffinose besides active ingredients of tender shoots, and has a unique health-care effect of tea tree roots of lowering blood cholesterol, triglyceride and the like; the innovative Xungen tea based on a production and brewing process can enrich products on the existing tea market.

Description

technical field [0001] The invention relates to a method for tissue culture of root-seeking tea, a production method and a rolling device, belonging to the field of tea. Background technique [0002] Tea drinks - tea is known as one of the three major beverages in the world, and has been cultivated extensively in China for a long time. Tea originated in China, and tea was first used as a sacrifice. However, it has been used as a vegetable by people since the late Spring and Autumn Period. It was developed into a medicinal drink in the middle of the Western Han Dynasty, and it was developed into a high-grade court drink in the late Western Han Dynasty. It was after the Western Jin Dynasty that it became popular among the people as an ordinary drink. The earliest relic of artificial tea plantation was found in the Tianluoshan site in Yuyao, Zhejiang Province, which has a history of more than 6,000 years. [0003] Tea contains catechin, cholesterolenone, caffeine, inositol, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A23F3/06A01G7/04A23F3/12A23F3/14
CPCA01H4/001A01H4/008A01G7/045A23F3/06A23F3/12A23F3/14Y02P60/14
Inventor 郑伟高雅徐晓丹吴森森
Owner KUNMING UNIV OF SCI & TECH
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