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Borojo monomer, extracts and preparation method and application of extracts

A technology of Baole fruit and extract, applied in the field of natural medicinal chemistry, can solve the problems of anti-inflammatory, anti-oxidative and hypoglycemic effects of monomer compounds that have not been reported in literature.

Active Publication Date: 2019-10-18
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that Bao Le Guo has anti-oxidation (Journal of International Pharmaceutical Research, 2013:817-821) and hypoglycemic activity. Anti-inflammatory, anti-oxidative and hypoglycemic effects of monomeric compounds

Method used

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  • Borojo monomer, extracts and preparation method and application of extracts
  • Borojo monomer, extracts and preparation method and application of extracts
  • Borojo monomer, extracts and preparation method and application of extracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 Baole fruit extract

[0058] Take 50kg of fresh Baole fruit, peel off the skin and seeds, add 200L of water to the pulp, stir, grind and mix well, and filter. Add 100L of water to the pomace, stir, grind and mix well, and filter.

[0059] The two filtrates were combined, extracted with 300 L of ethyl acetate, and the ethyl acetate layer was separated. Add 300L ethyl acetate to the aqueous layer, and separate the ethyl acetate layer. The aqueous layer was further extracted with 300L ethyl acetate, and the ethyl acetate layer was separated.

[0060] The three ethyl acetate layers were combined, concentrated to dryness under reduced pressure, and 650 g of Depotola fruit extract was obtained.

Embodiment 2

[0061] The preparation of embodiment 2 Baole fruit extract

[0062] Take 5kg of Baole fruit powder, add 50L of water, stir, mix well, and filter. Add 50L of water to the filter residue, stir, mix well, and filter.

[0063] The two filtrates were combined, extracted with 100 L of ethyl acetate, and the ethyl acetate layer was separated. Add 100L of ethyl acetate to the aqueous layer and extract 3 times, and separate the ethyl acetate layer each time.

[0064] The 4 ethyl acetate layers were combined, concentrated to dryness under reduced pressure, and 480 g of Depotola extract was obtained.

Embodiment 3

[0065] Example 3 Compound Preparation and Structure Identification

[0066]Add 500g of Baole fruit extract to redissolve in methanol, mix the sample with 80-100 mesh silica gel, then go through 200-300 mesh silica gel column chromatography, sequentially use 95:5, 90:10, 85:115, 80:20, 75 :25, 70:30, 60:40, 50:50, 0:100 petroleum ether-ethyl acetate gradient elution, according to TLC detection, combined fractions with similar thin-layer spots to obtain Fr.1-Fr. 9 total of 9 components.

[0067] Fr.4 was subjected to medium and low pressure ODS column chromatography, methanol-water gradient elution (90:10→100:0), and 7 sub-fractions (Fr.4a~Fr.4g) were obtained. Among them, Fr.4c, Fr.4d and Fr.4e were respectively subjected to Sephadex LH-20, Pre-HPLC and recrystallization to obtain the compounds Baole fruit acid 1 (171mg), Baole fruit acid 2 (163mg), and Baole fruit acid 7 (201mg) and Polyphenol 10 (167mg).

[0068] Fr.6 was subjected to medium and low pressure ODS column chr...

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PUM

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Abstract

The invention belongs to the technical field of natural pharmaceutical chemistry, and relates to Borojo acid 1 to Borojo acid 13 separated from South American fruit, namely Borojo, and a preparation method and application of the Borojo acid 1 to Borojo acid 13. The preparation method comprises the steps that Borojo mature fruits serve as raw materials, and are peeled and subjected to seed removing, water is added into pulp, even mixing and grinding into thick liquid are conducted, or water is added into Borojo freeze-dried powder, even mixing and filtering are conducted, ethyl acetate is addedinto a filtrate for extraction, an ethyl acetate layer is concentrated to be dry and then subjected to chromatographic separation technologies such as silicone, octadecylsilane chemically bonded silica (ODS), sephadex LH-20 and preparative high performance liquid chromatography (Pre-HPLC), and thirteen Borojo acid compounds are obtained through separation from the Borojo, wherein the Borojo acid1 to the Borojo acid 10 are new compounds, and the other three Borojo acid compounds are known compounds obtained through first separation from the Borojo. Experiments show that the prepared compoundshave activities of resisting oxidation, resisting inflammation and reducing blood glucose.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a monomeric compound, an extract, a preparation method and an application of a pomegranate. Background technique [0002] Bora fruit is the ripe fruit of Borojoa patinoi Cuatrec and Borojoa sorbilis Cuatrec. Distributed in the tropical rainforest of Ecuador in the Amazon basin, native to Colombia, Ecuador and Panama. Bao Le fruit is rich in fructose and glucose, water-soluble B vitamins, amino acids and proteins, which are of great development and utilization value. Local aborigines believe that eating Potato can eliminate fatigue, enhance immunity, strengthen yang, improve symptoms of respiratory inflammation and anti-inflammation. It has been reported in the literature that Bao Le Guo has anti-oxidation (Journal of International Pharmaceutical Research, 2013:817-821) and hypoglycemic activity. Anti-inflammatory, antioxidant and hypoglycemic effects of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/42C07C61/20C07C67/48C07C69/608C07C69/757C07C61/26C07D311/94C07D307/935A61P29/00A61P3/10A23L33/105
CPCC07C61/20C07C69/608C07C69/757C07C61/26C07D311/94C07D307/935A61P29/00A61P3/10A23L33/105A23V2002/00A23V2200/328A23V2200/302A23V2250/21
Inventor 叶文才范春林王英梅炬铭刘俊珊黄晓君许琳赵轩
Owner JINAN UNIVERSITY
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