A kind of immunomagnetic beads and preparation method thereof

An immune magnetic bead, uniform technology, applied in the direction of microsphere preparation, inductor/transformer/magnet manufacturing, magnetic objects, etc., can solve the problems of poor magnetic response, high price, uneven particle size, etc.

Active Publication Date: 2022-04-19
DONGGUAN DONGYANG SOLAR SCI RES & DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Foreign commercial magnetic beads are expensive, and the technology is strictly confidential. Domestic magnetic beads generally have defects such as uneven particle size, poor magnetic response, and inconsistent magnetic response. What is more serious is that nano-magnetic particles and microspheres appear during use. Shedding of contaminated samples

Method used

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  • A kind of immunomagnetic beads and preparation method thereof
  • A kind of immunomagnetic beads and preparation method thereof
  • A kind of immunomagnetic beads and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] 1. Swelling of polystyrene microspheres

[0071] Take 2g of polystyrene microspheres (1μm) and disperse them into 100mL of 0.1% SDS aqueous solution, then add 20mL of absolute ethanol and 5g of dibutyl phthalate, fully ultrasonically emulsify and add to a four-necked flask with a mechanical stirrer , stirred at a water bath temperature of 50° C. for 10 h, centrifuged to separate white solid particles after swelling, washed with pure water and absolute ethanol for 3 to 5 times, and then used for later use.

[0072] 2. Synthesis of magnetic composite microspheres

[0073] Dissolve 1.25g NaOH in 50mL EG, then add the swollen polystyrene microspheres into it, stir vigorously to disperse, and then perform ultrasonic to form a uniform emulsion to obtain emulsion A; take another 10mL EG, 5gFeCl 3 ·6H 2 O and 10mLH 2 O mixed with ultrasound to obtain solution B. Slowly add solution B to emulsion A under vigorous stirring. After the dropwise addition, continue to stir for 10 ...

Embodiment 2

[0084] Adjust the water consumption of solution B in the second step of Example 1 to 18mL, adjust the pressure to 0.6MPa, raise the temperature to 210°C, extend the reaction time to 20h, and keep other process steps, parameters and operations unchanged. Finally, the performance of the magnetic beads was obtained in the experiment. And structure is close to embodiment 1.

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Abstract

The invention provides an immune magnetic bead and a preparation method thereof, comprising the following steps: swelling polystyrene microspheres; synthesizing magnetic composite microspheres under high temperature and high pressure; removing exposed magnetic particles and magnetic particles on the surface of the magnetic composite microspheres by acid dissolution surface cross-linking modification. The immunomagnetic beads prepared by the method of the invention have uniform particle size, good magnetic response, stable structure and consistent magnetic content.

Description

technical field [0001] The invention relates to the technical field of composite material preparation, in particular to a preparation method of immunomagnetic beads. Background technique [0002] Magnetic beads are composite functional microspheres with superparamagnetism. Because of their surface modification with functional groups such as amino groups and carboxyl groups, they can be coupled to proteins, DNA, etc. Therefore, it has a wide range of applications in cell separation, immobilized enzymes, immunodiagnosis, tumor targeted therapy, and DNA extraction. [0003] Chemiluminescent immunomagnetic beads have higher requirements among all magnetic beads, and require suitable particle size, high particle size uniformity, magnetic response consistency, etc. for magnetic microspheres. Foreign commercial magnetic beads are expensive, and the technology is strictly confidential. Domestic magnetic beads generally have defects such as uneven particle size, poor magnetic respon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F257/02C08F212/36C08F220/06C08K3/22B01J13/14H01F1/00H01F41/00
CPCC08F257/02C08K3/22B01J13/14H01F1/0018H01F41/00C08K2003/2275C08K2201/011C08F212/36C08F220/06
Inventor 王鹏侯立威吴志能谌庆春李向东张军
Owner DONGGUAN DONGYANG SOLAR SCI RES & DEV CO LTD
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