Xinjiang redroot gromwell seed chemical germination effect judgment method and germination method

A technology of Xinjiang comfrey and determination method, applied in the agricultural field, can solve the problems of lack of reference significance and unscientific effect, and achieve the effect of promoting germination and growth

Inactive Publication Date: 2019-10-22
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the effects of these chemical germination methods are simply determined after the relevant growth indicators of the young shoots are detected after the seeds germinate.
In fact, the results obtained by this

Method used

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  • Xinjiang redroot gromwell seed chemical germination effect judgment method and germination method
  • Xinjiang redroot gromwell seed chemical germination effect judgment method and germination method
  • Xinjiang redroot gromwell seed chemical germination effect judgment method and germination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 SOD enzyme assay

[0049] Nitrogen blue tetrazolium photochemical reduction method (NBT method) was used to measure superoxide dismutase (SOD) activity, specifically:

[0050] (1) Reagent preparation

[0051] ①Preparation of 0.05mol / L phosphate buffer solution (PBS, pH7.8)

[0052] A mother liquor (0.2mol / L disodium hydrogen phosphate solution): take Na 2 HPO 4 12H 2 O (molecular weight 358.14) 71.7g, dilute to 1000mL with distilled water;

[0053] B mother liquor (0.2mol / L sodium dihydrogen phosphate solution): take NaH 2 PO 4 2H 2 O (molecular weight 156.01) 31.2g, dilute to 1000mL with distilled water.

[0054] Preparation of 0.05mol / LPBS (pH7.8): Take 228.75mL of A mother liquor and 21.25mL of B mother liquor, and dilute to 1000mL with distilled water.

[0055] ②130mmol / L methionine (Met) solution: Weigh 1.9399g Met, dissolve it in PBS and dilute to 100mL.

[0056] ③750 μmol / L nitrogen blue tetrazolium (NBT) solution: Weigh 0.06133 g of NBT, diss...

Embodiment 2

[0077] Example 2 POD enzyme assay

[0078] Adopt guaiacol method to measure peroxidase (POD) activity, specifically:

[0079] (1) Reagent preparation

[0080] ①Preparation of 0.2mol / L phosphate buffer solution (PBS, Ph7.0):

[0081] A mother liquor (0.2mol / L disodium hydrogen phosphate solution): take Na 2 HPO 4 12H 2 O (molecular weight 358.14) 71.7g, dilute to 1000mL with distilled water;

[0082] B mother liquor (0.2mol / L sodium dihydrogen phosphate solution): take NaH 2 PO 4 2H 2 O (molecular weight 156.01) 31.2g, dilute to 1000mL with distilled water.

[0083] Preparation of 0.2mol / L PBS (pH6.0): Take 123mL of A mother liquor and 877mL of B mother liquor and mix well.

[0084] ②Reaction mixture preparation:

[0085] Take 200mL PBS (0.2mol / L, pH6.0), add 0.076mL guaiacol (2-methoxyphenol) liquid (stock solution) and heat and stir to dissolve, add 0.112mL 30% H 2 o 2 , mix well and store in the refrigerator for later use.

[0086] (2) Sample determination

[0...

Embodiment 3

[0099] Example 3 CAT enzyme assay

[0100] Catalase (CAT) activity was measured by ultraviolet absorption method, specifically:

[0101] (1) Reagent preparation

[0102] ①Preparation of 0.15mol / L phosphate buffer solution (PBS, pH7.0):

[0103] A mother liquor (0.2mol / L disodium hydrogen phosphate solution): take Na 2 HPO 4 12H 2 O (molecular weight 358.14) 71.7g, dilute to 1000mL with distilled water;

[0104] B mother liquor (0.2mol / L sodium dihydrogen phosphate solution): take NaH 2 PO 4 2H 2 O (molecular weight 156.01) 31.2g, dilute to 1000mL with distilled water.

[0105] Preparation of 0.15mol / LPBS (pH7.0): Take 457.5mL of A mother liquor and 292.5mL of B mother liquor, mix well, and dilute to 1000mL with distilled water.

[0106] ②Reaction mixture preparation:

[0107] Take 200mL PBS (0.15mol / L, pH7.0), add 0.3092mL 30% H 2 o 2 (Original solution) Shake well.

[0108] (2) Sample determination

[0109] ① CAT enzyme solution extraction: same as SOD.

[0110...

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Abstract

The invention discloses a Xinjiang redroot gromwell seed chemical germination effect judgment method and a germination method. Xinjiang redroot gromwell seeds are treated by a set chemical method andthen germinated in an induced manner. The judgment method includes the steps: measuring SOD (superoxide dismutase), POD (peroxidase) catalase activity of seedlings every day, and measuring correlatedgrowth indexes of the seedlings in the process from seed germination to germination completing; counting measured correlated indexes, performing data processing on the correlated indexes, and judginggermination effect of a current chemical method according to data processing results. Optimal chemical induction germination conditions are determined by the judgment method, and Xinjiang redroot gromwell seeds are germinated in an induced manner according to the conditions. According to the method, Xinjiang redroot gromwell seed chemical germination effect can be most scientifically and accurately judged, judgment results show that the germination rate of the Xinjiang redroot gromwell seeds is best, growth vigor is better, enzyme activity is higher in 30-110mg/L gibberellin, and the gibberellin is suitable for serving as a seed treatment solvent before sowing.

Description

technical field [0001] The invention belongs to the field of agricultural technology, and in particular relates to a method for judging the chemical germination effect of Xinjiang comfrey seeds and a germination method of Xinjiang comfrey seeds. Background technique [0002] There are many means of germination of Xinjiang Zicao seeds, among which chemical germination is one of the very commonly used means, including Yu Qiuliang et al. [1] Method, Luo Huiying, etc. [2] Method, Li Cuixiang, etc. [3] , Li Hesheng [4] , crown, etc. [5] and Cheng Lulu [6] method. However, the effects of these chemical germination methods are simply determined after the relevant growth indicators of the young shoots are detected after the seeds germinate. In fact, the results obtained by this kind of judgment are not scientific and lack of reference significance. This is because even if the detection effect is good at the young bud stage, there will still be many problems in the later develo...

Claims

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Application Information

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IPC IPC(8): A01C1/02
CPCA01C1/025
Inventor 马生军孙磊朱广伟杨建波丁晓霞耿阳
Owner XINJIANG AGRI UNIV
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