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Photodynamic therapy nano material for reducing GSH content of tumors and preparation method of material

A technology of photodynamic therapy and nanomaterials, which can be used in anti-tumor drugs, medical preparations containing active ingredients, materials for wave energy or particle radiation treatment, etc. Achieving good tumor treatment effect, reducing antioxidant capacity and excellent biocompatibility

Inactive Publication Date: 2019-10-25
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the complex tumor microenvironment has seriously hindered the further clinical application of PDT. Therefore, in recent years, more and more photodynamic studies have been carried out around the tumor microenvironment.
The high expression of glutathione (GSH) in tumor cells is closely related to the specificity of cancer cells. Due to its good antioxidant and detoxification capabilities, cancer cells have ROS resistance, thus weakening the photodynamic therapy. Effect

Method used

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  • Photodynamic therapy nano material for reducing GSH content of tumors and preparation method of material
  • Photodynamic therapy nano material for reducing GSH content of tumors and preparation method of material
  • Photodynamic therapy nano material for reducing GSH content of tumors and preparation method of material

Examples

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Effect test

Embodiment 1

[0024] The average hydrated particle size of a photodynamic therapy nanomaterial for reducing tumor GSH content prepared in this example is 180nm-220nm;

[0025] The above-mentioned preparation method of photodynamic therapy nanomaterials for reducing the GSH content of tumors specifically includes the following steps:

[0026] Step 1, the preparation of nano alumina: 0.004Mol Fe 3+ Dissolve the metal salt in water, then add 0.005Mol (235μL, 21.28μMol) ethylenediamine, adjust the pH of the solution to 8, stir, react at 200°C until complete, centrifuge and wash to obtain nano-alumina AlO(OH );

[0027] Step 2. Preparation of biocompatible nano-metal particles: Take 1050 mg of nano-alumina AlO(OH) obtained in step 1 and disperse it in water by ultrasonic (ultrasonic power is 200 W, ultrasonic time is 20 min), add 15 mg (0.1 mM, 150 mL) Bovine serum albumin BSA, stirred until the reaction is complete, and obtained biocompatible nano-metal particles AlO(OH)@BSA after dialysis; ...

Embodiment 2

[0030] The average hydrated particle size of a photodynamic therapy nanomaterial for reducing tumor GSH content prepared in this example is 180nm-220nm;

[0031] The above-mentioned preparation method of photodynamic therapy nanomaterials for reducing the GSH content of tumors specifically includes the following steps:

[0032] Step 1, the preparation of nano-alumina: 0.003Mol Fe 3+ Dissolve the metal salt in water, then add 0.006Mol (300μL, 10μMol) ethylenediamine, adjust the pH of the solution to 7, stir, and react completely at 150°C (generally 10~15h), centrifuge and wash to obtain Nano-alumina AlO(OH);

[0033] Step 2. Preparation of biocompatible nano-metal particles: Take 600mg of nano-alumina AlO(OH) obtained in step 1 and disperse it in water by ultrasonic (100W ultrasonic power, 50min ultrasonic time), add 10mg (0.1mM, 100mL) Bovine serum albumin BSA, stirred until the reaction is complete, and obtained biocompatible nano-metal particles AlO(OH)@BSA after dialysis;...

Embodiment 3

[0036] The average hydrated particle size of a photodynamic therapy nanomaterial for reducing tumor GSH content prepared in this example is 180nm-220nm;

[0037] The above-mentioned preparation method of photodynamic therapy nanomaterials for reducing the GSH content of tumors specifically includes the following steps:

[0038] Step 1, the preparation of nano alumina: 0.005Mol Fe 3+ Dissolve the metal salt in water, then add 0.004Mol (400μL, 10μMol) ethylenediamine, adjust the pH of the solution to 10, stir, react at 250°C until complete, centrifuge and wash to obtain nano-alumina AlO(OH) ;

[0039] Step 2, preparation of biocompatible nano-metal particles: disperse 960mg of nano-alumina AlO(OH) obtained in step 1 in water by ultrasonic (ultrasonic power is 400W, ultrasonic time is 10min), add 12mg (0.1mM, 120mL) Bovine serum albumin BSA, stirred until the reaction is complete, and obtained biocompatible nano-metal particles AlO(OH)@BSA after dialysis;

[0040] Step 3. Format...

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Abstract

The invention provides a preparation method of a photodynamic therapy nano material for reducing GSH content of tumors. The method includes the following steps: (1) dissolving Fe3+ metal salt in water, adding ethylenediamine, adjusting the pH value of the solution to 7-10, stirring, reacting completely at 150-250 DEG C, centrifuging and washing to obtain nano alumina AlO(OH); (2) ultrasonically dispersing nano alumina AlO(OH) in water, adding bovine serum albumin, stirring until the reaction is complete, and dialyzing to obtain biocompatible nano metal particles AlO(OH)@BSA; and (3) dispersingbiocompatible nano metal particles AlO(OH)@BSA in distilled water, adding a photosensitizer, stirring, washing, centrifuging and dialyzing to obtain the final product. The photodynamic therapy nano material for reducing the GSH content of tumors can reduce the GSH content in tumor cells, so that the antioxidant capacity of tumor cells can be reduced, and the photodynamic therapy effect is improved.

Description

technical field [0001] The invention relates to the technical field of biological targeting nano-medicine, in particular to a photodynamic therapy nano-material for reducing tumor GSH content and a preparation method thereof. Background technique [0002] my country is one of the countries with high incidence of tumor diseases, and about a quarter of cancer deaths in the world occur in China. Deep tumors in the body, such as primary cellular hepatocellular carcinoma (HCC), ranks second among high-mortality tumors in my country. Most HCC patients in my country have a background of hepatitis B and liver cirrhosis. The onset is hidden, and early symptoms are mostly not obvious. Early detection The rate is low, surgical margins are difficult to define, postoperative recurrence rate is high, chemotherapy and postoperative chemotherapy are not highly targeted and toxic. Therefore, improving the targeting of deep tumor treatment, reducing skin phototoxicity with minimally invasive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/08A61K9/14A61K47/42A61P35/00
CPCA61K9/146A61K33/08A61K41/0071A61K47/42A61P35/00A61K2300/00
Inventor 徐祖顺刘豪杰
Owner HUBEI UNIV
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