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Immunogen for preparing mPR alpha monoclonal antibody

A monoclonal antibody, immunogen technology, applied in the direction of anti-animal/human immunoglobulin, antibody, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, etc., can solve the problem of undeveloped targeted drugs, etc. problem, to achieve the effect of high cost

Active Publication Date: 2019-10-25
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on functional progesterone membrane receptors in sperm, and no related targeted drugs have been developed.

Method used

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  • Immunogen for preparing mPR alpha monoclonal antibody
  • Immunogen for preparing mPR alpha monoclonal antibody
  • Immunogen for preparing mPR alpha monoclonal antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Design and preparation of embodiment 1mPRα monoclonal antibody

[0027] 1. Analysis and design of peptide sequences

[0028] Use DNAstar software to analyze the epitope of the amino acid of LRRC52, evaluate its hydrophilicity, antigenicity, surface possibility, flexible region and other indexes, comprehensively consider the amino acid category, distribution, structural complexity, easy oxidation difficulty, synthesis difficulty, small Based on the homology between mice and humans, etc., the 18 amino acids at positions 297-314 of mPRα were finally determined as the sequence of the synthetic polypeptide, and the corresponding sequence was YEARRPIYEP LHTHWPHN. Such as figure 1 , mPRα has 7 transmembrane structures, and this polypeptide is located between the 6th and 7th transmembrane regions, covering the entire extramembrane segment. The ACT396 automatic multi-channel peptide synthesizer is used to automatically synthesize the target peptide according to the compiled pr...

Embodiment 2

[0040] Example 2 mPRα monoclonal antibody inhibits the ability of human sperm to respond to progesterone

[0041] The mPRα monoclonal antibody was prepared as in Example 1.

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Abstract

The invention provides an immunogen for preparing an mPR alpha monoclonal antibody, and belongs to the technical field of biology. The immunogen is a polypeptide, and the amino acid sequence of the immunogen is YEARRPIYE PLHTHWPHN. The invention further provides a preparation method of the mPR alpha monoclonal antibody prepared by the immunogen, which is obtained by crosslinking the target polypeptide YEARRPIYE PLHTHWPHN with KLH, and then sequentially carrying out the steps of preparing immune mice through the immunogen, preparing hybridoma cells, screening fusion cells, cloning hybridoma cells, preparing the monoclonal antibody by an ascites method, identifying and purifying the monoclonal antibody and the like. The invention further provides an application of the mPR alpha monoclonal antibody in preparing sperm physiological regulation mechanism drugs or male contraceptives. The mPR alpha monoclonal antibody prepared by the immunogen has high valence and specificity, can specifically recognize mPR alpha, and can be a novel molecular target for regulating sperm function compounds and male contraceptives.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to an immunogen for preparing mPRα monoclonal antibody. Background technique [0002] Today, fertility issues are a worldwide health and social issue. On the other hand, the current global population has reached 7 billion. It is of great significance to find an ideal contraceptive method to actively control the population. Contraceptives in the current market are mainly hormonal drugs for women, and there is no marketable contraceptive drug for male reproductive physiology. Based on the side effects of existing female contraceptives and changes in people's reproductive ethics, there is a very broad market demand for the development of safe, effective, and easy-to-recover male contraceptives. Therefore, revealing the mechanism of male reproductive regulation at the molecular level has important social significance for studying the pathophysiological mechanism of male infertility, explorin...

Claims

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Application Information

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IPC IPC(8): C07K14/72C07K16/28A61K39/395A61P15/16
CPCA61P15/16C07K14/72C07K16/2869C07K2317/76
Inventor 罗韬曾旭辉陈厚仰彭真程一民
Owner NANCHANG UNIV
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