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A method for regulating and controlling the content of ascorbic acid in tomato fruit and its application

A technology of ascorbic acid and tomato, applied in the biological field, can solve the problems of high cost, complicated operation, unfavorable popularization and application by ordinary growers, etc., and achieve the effect of low cost, simple operation and easy technology

Active Publication Date: 2022-02-15
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of technical operation is complicated and costly, which is not conducive to popularization and application among ordinary growers.

Method used

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  • A method for regulating and controlling the content of ascorbic acid in tomato fruit and its application
  • A method for regulating and controlling the content of ascorbic acid in tomato fruit and its application
  • A method for regulating and controlling the content of ascorbic acid in tomato fruit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 Methylation Treatment of Tomato Seedlings and Adult Plants

[0042] 1. Methylation Treatment of Tomato Seedlings

[0043] Add 50 μL of 1 mM 5-azacytidine aqueous solution to the ordinary inoculation medium (the medium is a standard 1 / 2 MS medium formula, and obtain the MS medium formula according to the literature Murashige and Skoog, 1962); the control group added 50 μL H 2 O was added to an equal amount of the same inoculation medium and repeated 3 times. Seeds were grown on inoculation medium, and the leaves of the plants were taken every other week for four consecutive weeks. The removed leaves were stored in liquid nitrogen for a short period of time, and for long-term storage, they were stored in a -80°C refrigerator.

[0044] 2. Methylation treatment of adult tomato plants

[0045] Inject 50 μL of 1 mM 5-azaC aqueous solution at the flower stalk of the tomato during the flowering period, and inject only once, and control with 50 μL H 2 O process...

Embodiment 2

[0049] Example 2 AsA pathway bioinformatics and molecular biology analysis

[0050] Based on the research results of Example 1, the present invention analyzes at the gene level to explore the effect of methylation regulation on AsA content in tomato. The specific experimental operation is as follows:

[0051] Bioinformatics analysis: analysis process according to the method described before (Lang et al 2017): use trim to remove low-quality sequences (q<20) in BRAT-BW (Harris et al 2012), use BRAT-BW to remove Reads following low-quality data were mapped to the reference genome, allowing for two mismatches, and the reference genome version was SL2.50 (ftp: / / ftp.solgenomics.net / tomato genome / assembly / build_2.50 / ; Consortium 2012). Use the remove-dupl command of BRAT-BW to remove possible PCR duplications.

[0052] Methylation levels were assessed using the weighted methylation level algorithm proposed by Schultz et al. (Schultz et al2012). If an interval has 3 CHH sites: 3 ou...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a method for regulating and controlling the ascorbic acid content of tomato fruit and its application. The present invention found that the use of methyltransferase inhibitors to treat tomato tissue can significantly increase the content of ascorbic acid in tomato, and at the same time start with the key genes in the AsA pathway that have been identified in the laboratory, and study them through bioinformatics and molecular biology methods The effect of methylation regulation on AsA content in tomato was investigated. The invention provides a new method for regulating the content of ascorbic acid in tomatoes, and the method is simple in operation, low in cost, easy to popularize the technology, has good technical effect, and can significantly increase the content of ascorbic acid in tomatoes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for regulating and controlling the ascorbic acid content of tomato fruit and its application. Background technique [0002] Tomato (Solanum lycopersicum) is an important model plant in horticultural research, which is rich in nutrients such as ascorbic acid (AsA). Ascorbic acid (L-ascorbate acid, AsA), also known as vitamin C, is a strong antioxidant substance and one of the most abundant water-soluble low-molecular-weight antioxidants in plant cells. It plays a positive role in cell activity and plant growth. AsA provides electrons through oxidation-reduction reactions, has the function of anti-oxidation, and helps plants cope with adversity. As a cofactor of related enzymes in the process of plant growth and development, AsA is an important growth regulator. AsA also plays a very important role in protecting human health. It can remove free radicals that ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H3/04
CPCA01H3/04
Inventor 张余洋叶志彪郑伟张俊红张廷艳
Owner HUAZHONG AGRI UNIV
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